Grapevine leafroll-associated virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9L8F5|G9L8F5_9CLOS p24 OS=Grapevine leafroll-associated virus 1 OX=47985 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 5.49FAPVCYY8 pKa = 9.69ICTKK12 pKa = 10.65NEE14 pKa = 3.69EE15 pKa = 4.25GSDD18 pKa = 3.52KK19 pKa = 10.96KK20 pKa = 10.59LTLLSRR26 pKa = 11.84EE27 pKa = 4.17DD28 pKa = 4.95LIDD31 pKa = 4.13FNSDD35 pKa = 3.05EE36 pKa = 4.46NQDD39 pKa = 3.69EE40 pKa = 4.36LSLEE44 pKa = 3.94KK45 pKa = 10.79KK46 pKa = 10.23PFVKK50 pKa = 10.26SYY52 pKa = 11.07EE53 pKa = 3.93NYY55 pKa = 10.09LRR57 pKa = 11.84DD58 pKa = 3.15WAEE61 pKa = 3.66RR62 pKa = 11.84PFYY65 pKa = 10.33FSNAMSVMDD74 pKa = 4.73LAMHH78 pKa = 6.97TGNASIDD85 pKa = 3.76SDD87 pKa = 3.9SGLSKK92 pKa = 10.64SGEE95 pKa = 3.92KK96 pKa = 10.04RR97 pKa = 11.84RR98 pKa = 11.84TAVEE102 pKa = 4.01VQSPWKK108 pKa = 9.39TGIPFDD114 pKa = 4.26SVDD117 pKa = 3.46NGVLVAVGYY126 pKa = 10.34EE127 pKa = 4.03GLNADD132 pKa = 3.78KK133 pKa = 10.92KK134 pKa = 9.73KK135 pKa = 10.64ARR137 pKa = 11.84SVALFMQNEE146 pKa = 4.44GSYY149 pKa = 11.1IMLIGGEE156 pKa = 4.69VYY158 pKa = 10.74ASSSYY163 pKa = 10.54IKK165 pKa = 10.55EE166 pKa = 4.0CMRR169 pKa = 11.84CQRR172 pKa = 11.84LASTMSGDD180 pKa = 3.76VFVDD184 pKa = 3.51ALRR187 pKa = 11.84LVV189 pKa = 3.58

Molecular weight: 21.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9L8F1|G9L8F1_9CLOS Coat protein OS=Grapevine leafroll-associated virus 1 OX=47985 GN=CP PE=4 SV=1
MM1 pKa = 7.4NPLVWLTGATDD12 pKa = 3.55YY13 pKa = 11.1MSVLRR18 pKa = 11.84PLYY21 pKa = 10.36EE22 pKa = 4.16DD23 pKa = 4.65FKK25 pKa = 11.28AGGGRR30 pKa = 11.84RR31 pKa = 11.84VVNYY35 pKa = 10.07IEE37 pKa = 4.63VIDD40 pKa = 5.1RR41 pKa = 11.84GDD43 pKa = 3.3KK44 pKa = 9.24PHH46 pKa = 7.63IIRR49 pKa = 11.84GLSPSDD55 pKa = 3.43EE56 pKa = 4.18NFEE59 pKa = 4.64LIAWAVAVGAFEE71 pKa = 3.9NSIYY75 pKa = 11.07AFDD78 pKa = 4.96TILSTMRR85 pKa = 11.84NWKK88 pKa = 10.23AYY90 pKa = 6.83ATPGSLQLDD99 pKa = 3.79TPITKK104 pKa = 10.16KK105 pKa = 10.44KK106 pKa = 10.0NKK108 pKa = 9.73HH109 pKa = 4.32FTLTAEE115 pKa = 3.81QQAAVRR121 pKa = 11.84TSPNSTNEE129 pKa = 3.32AFAQLFLNLGVFLKK143 pKa = 10.38RR144 pKa = 11.84VPKK147 pKa = 10.82DD148 pKa = 3.23EE149 pKa = 5.67DD150 pKa = 3.07ISGRR154 pKa = 11.84GNIPLVRR161 pKa = 11.84VSQAEE166 pKa = 3.99STDD169 pKa = 3.72RR170 pKa = 11.84IEE172 pKa = 3.99QGRR175 pKa = 11.84YY176 pKa = 9.45SPLLAKK182 pKa = 9.8FVRR185 pKa = 11.84KK186 pKa = 7.94YY187 pKa = 7.68THH189 pKa = 6.49VVDD192 pKa = 5.59AYY194 pKa = 10.64DD195 pKa = 4.36SVVTRR200 pKa = 11.84QKK202 pKa = 10.61IEE204 pKa = 3.73VCSYY208 pKa = 9.47YY209 pKa = 10.03IPEE212 pKa = 5.14LIGLLFRR219 pKa = 11.84ARR221 pKa = 11.84DD222 pKa = 3.26GAQAYY227 pKa = 6.65KK228 pKa = 10.05TVSLYY233 pKa = 10.7LASFLQSKK241 pKa = 10.79DD242 pKa = 3.05PGKK245 pKa = 10.5YY246 pKa = 9.92DD247 pKa = 3.28PEE249 pKa = 4.12TRR251 pKa = 11.84VIWFARR257 pKa = 11.84TLTMARR263 pKa = 11.84EE264 pKa = 3.99LLEE267 pKa = 3.68IYY269 pKa = 10.71YY270 pKa = 9.95NIRR273 pKa = 11.84VEE275 pKa = 4.0VSEE278 pKa = 4.8RR279 pKa = 11.84EE280 pKa = 3.74IIRR283 pKa = 11.84NFRR286 pKa = 11.84IQPGDD291 pKa = 3.62EE292 pKa = 3.91FHH294 pKa = 6.72QLSTMLLGLVADD306 pKa = 5.05TSAKK310 pKa = 9.23LTGITDD316 pKa = 3.11VDD318 pKa = 3.94VVLRR322 pKa = 11.84RR323 pKa = 11.84VLGLCLKK330 pKa = 11.08AMVDD334 pKa = 4.07EE335 pKa = 4.73NQHH338 pKa = 5.67ISNINYY344 pKa = 8.45TGALYY349 pKa = 11.22LMLVYY354 pKa = 9.48FSIVGTNYY362 pKa = 9.72LRR364 pKa = 11.84KK365 pKa = 9.55KK366 pKa = 9.11DD367 pKa = 3.99TPNEE371 pKa = 3.73MVFRR375 pKa = 11.84INGVVRR381 pKa = 11.84KK382 pKa = 10.48VNFTHH387 pKa = 7.75LKK389 pKa = 9.77RR390 pKa = 11.84LVAEE394 pKa = 4.33ASVDD398 pKa = 3.01GRR400 pKa = 11.84NYY402 pKa = 9.37PRR404 pKa = 11.84EE405 pKa = 3.91ISSVFAGITLQLRR418 pKa = 11.84QLGNIDD424 pKa = 3.57TVVWPDD430 pKa = 3.43VVLSNPSLGFDD441 pKa = 3.6TVLHH445 pKa = 6.18SGYY448 pKa = 10.19ISLNPAMYY456 pKa = 10.5KK457 pKa = 10.28DD458 pKa = 3.25IEE460 pKa = 4.37IIKK463 pKa = 10.25RR464 pKa = 11.84KK465 pKa = 9.18IRR467 pKa = 11.84NSTHH471 pKa = 4.88WVGGYY476 pKa = 10.32KK477 pKa = 9.83YY478 pKa = 10.72GKK480 pKa = 10.09RR481 pKa = 11.84FF482 pKa = 3.22

Molecular weight: 54.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5466

59

2204

546.6

60.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.245 ± 0.415

1.5 ± 0.317

5.671 ± 0.268

6.092 ± 0.255

4.409 ± 0.364

6.604 ± 0.357

1.518 ± 0.18

5.123 ± 0.453

6.495 ± 0.359

9.239 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.128

4.135 ± 0.414

3.531 ± 0.319

2.47 ± 0.176

6.147 ± 0.284

8.361 ± 0.27

5.507 ± 0.309

9.276 ± 0.305

0.732 ± 0.095

3.805 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski