[Clostridium] ultunense Esp

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Schnuerera; Schnuerera ultunensis

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1ZI51|M1ZI51_9FIRM Uncharacterized protein OS=[Clostridium] ultunense Esp OX=1288971 GN=CUESP1_0680 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.37KK3 pKa = 10.32RR4 pKa = 11.84ILSLTLVICLLISLLVGCGQKK25 pKa = 10.86DD26 pKa = 3.41SDD28 pKa = 4.71SEE30 pKa = 4.54SDD32 pKa = 3.58TPSTGNAAEE41 pKa = 4.93DD42 pKa = 4.08GVKK45 pKa = 10.18FAVLFGTGGLGDD57 pKa = 3.5NGYY60 pKa = 10.58NDD62 pKa = 4.03EE63 pKa = 4.35VYY65 pKa = 10.17KK66 pKa = 10.05GCEE69 pKa = 3.69MAVEE73 pKa = 3.95QLDD76 pKa = 3.64ASFDD80 pKa = 3.64YY81 pKa = 11.22CEE83 pKa = 4.61PKK85 pKa = 10.55DD86 pKa = 3.63VAEE89 pKa = 5.29FEE91 pKa = 4.48TQLRR95 pKa = 11.84SYY97 pKa = 10.63ADD99 pKa = 3.2SGEE102 pKa = 3.96YY103 pKa = 10.37DD104 pKa = 3.78VIIAISTEE112 pKa = 3.99QVDD115 pKa = 3.98ALKK118 pKa = 10.1MVAEE122 pKa = 5.4EE123 pKa = 4.09YY124 pKa = 10.32QDD126 pKa = 4.36QKK128 pKa = 11.61FCMLDD133 pKa = 3.67TIVEE137 pKa = 4.62GYY139 pKa = 11.23DD140 pKa = 3.48NIHH143 pKa = 6.81SISAAYY149 pKa = 8.88PDD151 pKa = 3.48QHH153 pKa = 6.52FLSGMLAGLATQDD166 pKa = 3.44EE167 pKa = 5.18RR168 pKa = 11.84FPLSNPEE175 pKa = 3.91NVLGFCIAMDD185 pKa = 3.99TPTSRR190 pKa = 11.84GQAAGFIAGAKK201 pKa = 8.69YY202 pKa = 10.57VNPDD206 pKa = 3.14VEE208 pKa = 4.36ILTNYY213 pKa = 8.85IGAYY217 pKa = 9.61NDD219 pKa = 3.54PATAKK224 pKa = 9.96EE225 pKa = 4.2LALMMYY231 pKa = 9.93EE232 pKa = 4.19RR233 pKa = 11.84GADD236 pKa = 2.94IVSANAGSSTQGVFYY251 pKa = 10.5AAEE254 pKa = 4.03EE255 pKa = 3.98KK256 pKa = 10.43DD257 pKa = 3.46RR258 pKa = 11.84YY259 pKa = 10.78VIGTSLAMADD269 pKa = 4.56PDD271 pKa = 4.43HH272 pKa = 6.79SLCTSLKK279 pKa = 9.74KK280 pKa = 10.4VWLFVVQEE288 pKa = 4.19IEE290 pKa = 4.23SLQNGTWEE298 pKa = 4.3PGNTVMGIPEE308 pKa = 4.72GVCNYY313 pKa = 10.26DD314 pKa = 3.16IEE316 pKa = 4.55GLNVEE321 pKa = 4.11IPEE324 pKa = 5.01DD325 pKa = 3.17IVAILDD331 pKa = 3.54EE332 pKa = 4.45ARR334 pKa = 11.84EE335 pKa = 4.14LIANGDD341 pKa = 3.86LVLPTDD347 pKa = 5.08LDD349 pKa = 4.79QIDD352 pKa = 3.65EE353 pKa = 4.36WASQNQYY360 pKa = 10.92NKK362 pKa = 10.86

Molecular weight: 39.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1ZLZ7|M1ZLZ7_9FIRM Uncharacterized protein OS=[Clostridium] ultunense Esp OX=1288971 GN=CUESP1_0681 PE=4 SV=1
MM1 pKa = 7.39NNNGFRR7 pKa = 11.84NGALLGSILGASLGMFFGTRR27 pKa = 11.84MGPMQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84IMRR38 pKa = 11.84TAKK41 pKa = 10.02RR42 pKa = 11.84AKK44 pKa = 8.88STLLNGMNSLWGG56 pKa = 3.62

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3144

0

3144

870061

20

2452

276.7

31.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.39 ± 0.048

0.857 ± 0.016

5.658 ± 0.037

7.924 ± 0.053

4.239 ± 0.031

6.904 ± 0.047

1.485 ± 0.016

10.441 ± 0.054

8.485 ± 0.042

9.373 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.02

5.645 ± 0.041

3.159 ± 0.027

2.285 ± 0.023

3.987 ± 0.03

5.683 ± 0.037

4.767 ± 0.029

6.196 ± 0.041

0.685 ± 0.014

4.138 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski