Aspergillus sclerotiicarbonarius (strain CBS 121057 / IBT 28362)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus sclerotiicarbonarius

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A319EYX3|A0A319EYX3_ASPSB Plasma membrane proteolipid 3 OS=Aspergillus sclerotiicarbonarius (strain CBS 121057 / IBT 28362) OX=1448318 GN=BO78DRAFT_237843 PE=3 SV=1
MM1 pKa = 7.14KK2 pKa = 9.74TYY4 pKa = 11.16SLLSTVAILGSFPFASTKK22 pKa = 9.91TINFVAHH29 pKa = 6.55QDD31 pKa = 3.57DD32 pKa = 4.63DD33 pKa = 5.01LLFLSPDD40 pKa = 4.47LIRR43 pKa = 11.84DD44 pKa = 3.61ILSGEE49 pKa = 4.2PVRR52 pKa = 11.84TVYY55 pKa = 9.34LTAGDD60 pKa = 4.09AGLGSDD66 pKa = 3.3YY67 pKa = 10.84WLGRR71 pKa = 11.84EE72 pKa = 4.41HH73 pKa = 7.52GSQAAYY79 pKa = 10.84ARR81 pKa = 11.84MSVASNTWIEE91 pKa = 3.99SDD93 pKa = 4.72AGIDD97 pKa = 3.99DD98 pKa = 4.7KK99 pKa = 11.69DD100 pKa = 3.25ITLYY104 pKa = 10.28TLQDD108 pKa = 3.66DD109 pKa = 4.29QNISLVFMHH118 pKa = 6.99LPDD121 pKa = 4.75GNLDD125 pKa = 3.72GTGFAADD132 pKa = 4.33EE133 pKa = 4.18NDD135 pKa = 3.82SLEE138 pKa = 4.16KK139 pKa = 10.66LWDD142 pKa = 3.76GSIDD146 pKa = 3.85EE147 pKa = 4.84IRR149 pKa = 11.84TIDD152 pKa = 4.11GSGTTYY158 pKa = 10.52TRR160 pKa = 11.84DD161 pKa = 3.35EE162 pKa = 4.33LLEE165 pKa = 4.35TIAALIDD172 pKa = 4.0DD173 pKa = 4.97FEE175 pKa = 6.6PDD177 pKa = 3.28EE178 pKa = 4.74VKK180 pKa = 10.29TGDD183 pKa = 3.6YY184 pKa = 11.43VNDD187 pKa = 4.35FGDD190 pKa = 4.38GDD192 pKa = 4.1HH193 pKa = 7.19SDD195 pKa = 4.11HH196 pKa = 6.56YY197 pKa = 10.19ATGYY201 pKa = 9.57FVNNALQASDD211 pKa = 4.17SNASLVGYY219 pKa = 9.43YY220 pKa = 10.2GYY222 pKa = 9.46PIQDD226 pKa = 2.81MDD228 pKa = 4.05VNLNGSDD235 pKa = 4.39IEE237 pKa = 4.39DD238 pKa = 3.53KK239 pKa = 11.27TNIFYY244 pKa = 10.62EE245 pKa = 4.27YY246 pKa = 10.59AAYY249 pKa = 8.07DD250 pKa = 3.6TATCSTADD258 pKa = 3.52SCSSRR263 pKa = 11.84PEE265 pKa = 4.02SSWLQRR271 pKa = 11.84EE272 pKa = 4.44YY273 pKa = 11.1EE274 pKa = 4.24VV275 pKa = 4.24

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A319ELJ4|A0A319ELJ4_ASPSB Bifunctional cytochrome P450/NADPH--P450 reductase OS=Aspergillus sclerotiicarbonarius (strain CBS 121057 / IBT 28362) OX=1448318 GN=BO78DRAFT_366756 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.54LAKK15 pKa = 9.74AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12562

0

12562

5900208

49

9581

469.7

52.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.458 ± 0.02

1.344 ± 0.009

5.6 ± 0.017

6.008 ± 0.021

3.773 ± 0.014

6.89 ± 0.021

2.498 ± 0.009

4.947 ± 0.017

4.239 ± 0.019

9.255 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.007

3.474 ± 0.011

6.14 ± 0.026

4.008 ± 0.016

6.162 ± 0.02

8.222 ± 0.024

6.047 ± 0.015

6.287 ± 0.016

1.542 ± 0.008

2.904 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski