Pseudomonas phage vB_PaS_IME307

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DJN5|A0A514DJN5_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaS_IME307 OX=2591408 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 10.47NNVVKK8 pKa = 10.78AQGTDD13 pKa = 3.15LYY15 pKa = 11.16FIDD18 pKa = 5.53PDD20 pKa = 3.68THH22 pKa = 7.45VVMNAGCITSLSGIDD37 pKa = 3.45TSIDD41 pKa = 3.62QIEE44 pKa = 4.55TTCLNEE50 pKa = 3.88TARR53 pKa = 11.84SYY55 pKa = 11.2VAGLATPGTATFSINTNPQDD75 pKa = 3.65PVHH78 pKa = 6.59IRR80 pKa = 11.84LLEE83 pKa = 4.07LKK85 pKa = 10.26NAGVSLDD92 pKa = 3.65WAVGWSDD99 pKa = 3.43GTSAPTAVLDD109 pKa = 4.32SSGEE113 pKa = 3.85YY114 pKa = 10.85DD115 pKa = 3.58FDD117 pKa = 4.11VPADD121 pKa = 4.13RR122 pKa = 11.84SWLLFEE128 pKa = 6.03GYY130 pKa = 9.22MNSFSFEE137 pKa = 3.86FAQNAVVTSSIGIQVSGEE155 pKa = 3.78PVLIPKK161 pKa = 8.07STSS164 pKa = 2.76

Molecular weight: 17.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DJN4|A0A514DJN4_9CAUD Tail fibers protein OS=Pseudomonas phage vB_PaS_IME307 OX=2591408 PE=4 SV=1
MM1 pKa = 7.51GGDD4 pKa = 3.43PKK6 pKa = 10.26IAYY9 pKa = 10.09RR10 pKa = 11.84MTPAQCKK17 pKa = 9.44RR18 pKa = 11.84YY19 pKa = 7.49MDD21 pKa = 4.31RR22 pKa = 11.84VISMYY27 pKa = 10.21RR28 pKa = 11.84RR29 pKa = 11.84QLVIHH34 pKa = 6.69AVGLPLVLSFFAFLVVKK51 pKa = 10.14LGWW54 pKa = 3.56

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

15937

37

917

164.3

18.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.78 ± 0.404

1.55 ± 0.161

5.867 ± 0.234

7.028 ± 0.29

3.439 ± 0.207

7.461 ± 0.208

1.744 ± 0.151

5.641 ± 0.215

4.894 ± 0.269

8.007 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.147

3.326 ± 0.222

4.624 ± 0.272

4.01 ± 0.268

6.319 ± 0.259

6.337 ± 0.246

4.926 ± 0.298

6.519 ± 0.208

2.02 ± 0.144

2.842 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski