Mycobacterium numidiamassiliense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U3PI29|A0A2U3PI29_9MYCO Uncharacterized protein OS=Mycobacterium numidiamassiliense OX=1841861 GN=MNAB215_5585 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 8.75KK4 pKa = 7.37TTDD7 pKa = 3.65PASPLYY13 pKa = 10.21QADD16 pKa = 3.21ITGVGFLPSDD26 pKa = 3.67SMIDD30 pKa = 3.6DD31 pKa = 4.83SIGSVPATMDD41 pKa = 3.44YY42 pKa = 11.19AQTDD46 pKa = 3.89PQVDD50 pKa = 3.45AGTDD54 pKa = 3.43MVVPAPLPGSIATHH68 pKa = 6.27IEE70 pKa = 4.02PTEE73 pKa = 3.82SDD75 pKa = 4.14YY76 pKa = 11.31NTLSDD81 pKa = 4.44HH82 pKa = 7.21RR83 pKa = 11.84YY84 pKa = 8.11TMLGDD89 pKa = 3.8DD90 pKa = 3.9

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U3P723|A0A2U3P723_9MYCO UPF0232 protein MNAB215_1731 OS=Mycobacterium numidiamassiliense OX=1841861 GN=MNAB215_1731 PE=3 SV=1
VV1 pKa = 7.22AKK3 pKa = 10.35GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 10.26GRR42 pKa = 11.84GALSAA47 pKa = 4.3

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5720

0

5720

1842979

22

10324

322.2

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.823 ± 0.048

0.839 ± 0.01

6.271 ± 0.029

5.196 ± 0.03

3.116 ± 0.017

8.81 ± 0.026

2.278 ± 0.016

4.416 ± 0.019

2.276 ± 0.02

9.778 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.015

2.38 ± 0.018

5.881 ± 0.028

3.147 ± 0.015

7.021 ± 0.029

5.599 ± 0.02

5.904 ± 0.02

8.567 ± 0.031

1.529 ± 0.012

2.205 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski