Pseudorhodobacter sp. E13

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudorhodobacter; unclassified Pseudorhodobacter

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S1AMU9|A0A3S1AMU9_9RHOB Methyltransferase OS=Pseudorhodobacter sp. E13 OX=2487931 GN=EGN72_11845 PE=3 SV=1
MM1 pKa = 6.52VTNTNTNPGAQTNQTITASSGTDD24 pKa = 3.05TLTGGLGNDD33 pKa = 4.76TINGLAGNDD42 pKa = 3.7FLRR45 pKa = 11.84GDD47 pKa = 4.13GAVQGAWHH55 pKa = 6.7YY56 pKa = 7.58EE57 pKa = 3.79TFNYY61 pKa = 10.11NFSSANGQAFTPAAFTTATRR81 pKa = 11.84TGSGYY86 pKa = 8.81VTDD89 pKa = 4.47FDD91 pKa = 4.53EE92 pKa = 5.88GGITNTMRR100 pKa = 11.84GVAASTNPEE109 pKa = 4.15DD110 pKa = 3.83FGVVYY115 pKa = 10.29TSTLNVTTGGTYY127 pKa = 10.75RR128 pKa = 11.84LTTSSDD134 pKa = 3.42DD135 pKa = 3.49GSTVQIFNSAGVPLNFSNQTGGTLNYY161 pKa = 10.06LNNDD165 pKa = 3.62FHH167 pKa = 8.64QSTTTRR173 pKa = 11.84WGDD176 pKa = 3.3VVLAPGQTYY185 pKa = 8.59TIQIRR190 pKa = 11.84YY191 pKa = 7.76WEE193 pKa = 4.21NLGADD198 pKa = 3.78TLAATINGPDD208 pKa = 3.38TGGATQNLLTSPMLGLPPGPSYY230 pKa = 11.37SVTGTAMGVEE240 pKa = 4.6GNDD243 pKa = 3.59VLDD246 pKa = 4.55GGAGNDD252 pKa = 3.84TIYY255 pKa = 11.3GDD257 pKa = 4.05GGNDD261 pKa = 3.49SLLGGADD268 pKa = 3.39NDD270 pKa = 4.11LLYY273 pKa = 11.13GGTGNDD279 pKa = 3.37TLFGGTGNDD288 pKa = 3.31TLYY291 pKa = 11.33GDD293 pKa = 5.17DD294 pKa = 5.67SSDD297 pKa = 4.13SLNGDD302 pKa = 3.85DD303 pKa = 5.81GTDD306 pKa = 3.5LLYY309 pKa = 11.19GGAGNDD315 pKa = 3.89TLNGGAGADD324 pKa = 3.65TLYY327 pKa = 11.32GDD329 pKa = 5.41DD330 pKa = 4.93GNDD333 pKa = 3.55SLRR336 pKa = 11.84GDD338 pKa = 4.03ADD340 pKa = 3.6NDD342 pKa = 3.99TLYY345 pKa = 11.28GGAGLDD351 pKa = 3.89TLNGDD356 pKa = 4.52DD357 pKa = 5.82GNDD360 pKa = 3.52ALYY363 pKa = 10.92GGADD367 pKa = 3.5ADD369 pKa = 3.86QLFGGLGSDD378 pKa = 3.84SLFGGTGADD387 pKa = 3.18TLYY390 pKa = 11.08GGDD393 pKa = 4.06GADD396 pKa = 4.22SLSGDD401 pKa = 4.6DD402 pKa = 5.35GNDD405 pKa = 3.32SLFGGLGDD413 pKa = 3.96DD414 pKa = 4.07TLLGGLGNDD423 pKa = 3.76TLYY426 pKa = 11.43GDD428 pKa = 5.45DD429 pKa = 5.76GNDD432 pKa = 3.73SLNGDD437 pKa = 4.3AGNDD441 pKa = 3.69SLFGGAGNDD450 pKa = 3.84TLNGGAGNNTLTGGAGNDD468 pKa = 3.05RR469 pKa = 11.84FIYY472 pKa = 9.98TIGSNLTISDD482 pKa = 4.47LNLGNSGGILDD493 pKa = 5.19GDD495 pKa = 3.68QTNNDD500 pKa = 4.04FLDD503 pKa = 4.06LSAFYY508 pKa = 10.85TNLNEE513 pKa = 4.89LRR515 pKa = 11.84DD516 pKa = 4.19DD517 pKa = 4.29FSDD520 pKa = 5.28DD521 pKa = 4.17GILNQSVGDD530 pKa = 3.98YY531 pKa = 11.08SDD533 pKa = 3.36NTALGGSIILTGITRR548 pKa = 11.84TDD550 pKa = 3.25LTTDD554 pKa = 4.23NINVACFTAGTQIEE568 pKa = 4.67TADD571 pKa = 3.89GPALIEE577 pKa = 4.25SLTAGTLIRR586 pKa = 11.84TLDD589 pKa = 3.56HH590 pKa = 6.75GLQPLRR596 pKa = 11.84AVLTRR601 pKa = 11.84SVPGDD606 pKa = 3.28GRR608 pKa = 11.84FAPIRR613 pKa = 11.84FHH615 pKa = 7.66AGALGNHH622 pKa = 6.43RR623 pKa = 11.84AFSTSPAHH631 pKa = 7.21RR632 pKa = 11.84MLIADD637 pKa = 4.09WRR639 pKa = 11.84AEE641 pKa = 3.86LLFGEE646 pKa = 4.54SEE648 pKa = 4.38VLISARR654 pKa = 11.84ALVNDD659 pKa = 4.08CTITRR664 pKa = 11.84APCDD668 pKa = 2.99RR669 pKa = 11.84VTYY672 pKa = 10.01YY673 pKa = 10.94HH674 pKa = 7.17LLLDD678 pKa = 3.31RR679 pKa = 11.84HH680 pKa = 6.18EE681 pKa = 5.06IIFAEE686 pKa = 5.6GIATEE691 pKa = 4.88SYY693 pKa = 9.33QHH695 pKa = 6.59GAFADD700 pKa = 3.98DD701 pKa = 4.35PGVEE705 pKa = 4.25AEE707 pKa = 4.98LDD709 pKa = 3.54ALFPGLLPAPSDD721 pKa = 3.52SARR724 pKa = 11.84PSLRR728 pKa = 11.84GYY730 pKa = 9.16EE731 pKa = 3.98AAALLSLPQRR741 pKa = 11.84RR742 pKa = 11.84AAA744 pKa = 4.17

Molecular weight: 76.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0YYZ7|A0A3S0YYZ7_9RHOB DUF1501 domain-containing protein OS=Pseudorhodobacter sp. E13 OX=2487931 GN=EGN72_07320 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.33GGRR28 pKa = 11.84IVLNNRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3709

0

3709

1156015

25

3543

311.7

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.292 ± 0.061

0.887 ± 0.013

5.736 ± 0.046

5.418 ± 0.037

3.716 ± 0.028

8.787 ± 0.053

2.005 ± 0.02

5.008 ± 0.026

3.342 ± 0.038

10.282 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.024

2.582 ± 0.026

5.209 ± 0.037

3.319 ± 0.019

6.506 ± 0.044

4.99 ± 0.031

5.492 ± 0.051

7.067 ± 0.034

1.399 ± 0.017

2.182 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski