Burkholderia phage BcepSauron

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Sarumanvirus; Burkholderia virus BcepSauron

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MLG1|A0A482MLG1_9CAUD Uncharacterized protein OS=Burkholderia phage BcepSauron OX=2530033 GN=BcepSauron_075 PE=4 SV=1
MM1 pKa = 7.9SDD3 pKa = 4.15LDD5 pKa = 4.02LSADD9 pKa = 4.06IINVSDD15 pKa = 2.98ICDD18 pKa = 3.45RR19 pKa = 11.84YY20 pKa = 11.3DD21 pKa = 3.41EE22 pKa = 5.24LKK24 pKa = 10.9DD25 pKa = 3.62EE26 pKa = 5.14RR27 pKa = 11.84DD28 pKa = 3.49THH30 pKa = 8.01DD31 pKa = 4.26SDD33 pKa = 5.57DD34 pKa = 4.8DD35 pKa = 4.33RR36 pKa = 11.84AADD39 pKa = 3.54WADD42 pKa = 3.79EE43 pKa = 4.31NPDD46 pKa = 3.38EE47 pKa = 5.0AVEE50 pKa = 4.17LEE52 pKa = 4.09QLEE55 pKa = 4.84KK56 pKa = 10.62ILTEE60 pKa = 4.24LNGEE64 pKa = 4.52GGDD67 pKa = 3.77HH68 pKa = 5.81QYY70 pKa = 11.09EE71 pKa = 4.66GNWYY75 pKa = 8.17PGCLIRR81 pKa = 11.84DD82 pKa = 3.67SHH84 pKa = 5.01FTEE87 pKa = 4.57YY88 pKa = 10.85CEE90 pKa = 4.88QLVSDD95 pKa = 4.26IGDD98 pKa = 3.64MPRR101 pKa = 11.84EE102 pKa = 3.92IPSYY106 pKa = 10.99LVIDD110 pKa = 4.17WDD112 pKa = 3.82ATAKK116 pKa = 10.43NLSVDD121 pKa = 3.28YY122 pKa = 9.88STIDD126 pKa = 3.25IEE128 pKa = 4.43GTEE131 pKa = 3.88YY132 pKa = 10.92LYY134 pKa = 10.99RR135 pKa = 5.67

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MLM0|A0A482MLM0_9CAUD DNA topoisomerase (ATP-hydrolyzing) OS=Burkholderia phage BcepSauron OX=2530033 GN=BcepSauron_169 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.14RR3 pKa = 11.84VVVVGAGPGVARR15 pKa = 11.84GLGRR19 pKa = 11.84VLGIDD24 pKa = 3.17TCVVNKK30 pKa = 10.47AVTQSLYY37 pKa = 11.02SIAACQSADD46 pKa = 3.26EE47 pKa = 4.5EE48 pKa = 4.32IRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 8.16TFHH55 pKa = 5.71QQFSGAYY62 pKa = 5.32GTRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 10.02KK68 pKa = 10.49RR69 pKa = 3.34

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

446

0

446

80383

30

1534

180.2

20.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.292 ± 0.177

1.071 ± 0.052

6.285 ± 0.126

6.373 ± 0.182

3.541 ± 0.089

6.871 ± 0.156

2.305 ± 0.086

4.795 ± 0.088

4.98 ± 0.201

8.056 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.063

3.891 ± 0.121

4.461 ± 0.1

3.778 ± 0.089

6.495 ± 0.163

5.653 ± 0.144

6.187 ± 0.192

7.279 ± 0.125

1.473 ± 0.05

3.465 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski