Lentibacillus sp. CBA3610

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lentibacillus; unclassified Lentibacillus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3575 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H8S1S4|A0A7H8S1S4_9BACI Response regulator OS=Lentibacillus sp. CBA3610 OX=2518176 GN=Len3610_01255 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.25KK3 pKa = 10.51CIWTVLIGTAILLFISACGDD23 pKa = 3.26NSNGGTADD31 pKa = 3.32EE32 pKa = 4.18NAEE35 pKa = 4.04NEE37 pKa = 4.21EE38 pKa = 4.05PAVLEE43 pKa = 4.32VEE45 pKa = 4.24FDD47 pKa = 3.7LPEE50 pKa = 4.11SAEE53 pKa = 3.96AGEE56 pKa = 4.32TVEE59 pKa = 5.6LKK61 pKa = 10.29ATVTYY66 pKa = 10.78GDD68 pKa = 4.56EE69 pKa = 4.15LVEE72 pKa = 4.67DD73 pKa = 3.98ADD75 pKa = 3.84EE76 pKa = 4.43VVFEE80 pKa = 4.43HH81 pKa = 6.89WEE83 pKa = 3.81QGNEE87 pKa = 4.01DD88 pKa = 4.34DD89 pKa = 4.09STMVEE94 pKa = 3.94ATNNEE99 pKa = 4.31DD100 pKa = 2.96GTYY103 pKa = 9.12TADD106 pKa = 3.46VSFDD110 pKa = 3.47EE111 pKa = 5.0DD112 pKa = 3.62GVYY115 pKa = 10.8EE116 pKa = 4.38MYY118 pKa = 11.27AHH120 pKa = 5.46TTARR124 pKa = 11.84EE125 pKa = 3.87MHH127 pKa = 6.09TMPKK131 pKa = 10.19KK132 pKa = 10.78SITIGDD138 pKa = 3.83GGSQEE143 pKa = 4.4SEE145 pKa = 4.18NGDD148 pKa = 3.58EE149 pKa = 4.37SQDD152 pKa = 3.62ASGHH156 pKa = 4.54EE157 pKa = 4.58HH158 pKa = 7.26GDD160 pKa = 3.57HH161 pKa = 7.48ADD163 pKa = 3.58GFSMDD168 pKa = 4.16FAQSEE173 pKa = 4.53NVSSGKK179 pKa = 8.35EE180 pKa = 3.66TDD182 pKa = 3.47LTVQLQMDD190 pKa = 4.5NEE192 pKa = 4.23PLEE195 pKa = 4.44EE196 pKa = 4.72ANVQYY201 pKa = 10.8EE202 pKa = 4.24ITNDD206 pKa = 3.5DD207 pKa = 3.87LDD209 pKa = 3.49IHH211 pKa = 6.41EE212 pKa = 4.4WAEE215 pKa = 3.97TDD217 pKa = 3.17EE218 pKa = 4.62TEE220 pKa = 4.05AGEE223 pKa = 4.3YY224 pKa = 8.39TGSYY228 pKa = 10.11SFEE231 pKa = 3.89EE232 pKa = 4.2AGTYY236 pKa = 11.11NMTIHH241 pKa = 6.41VKK243 pKa = 10.6NDD245 pKa = 3.58DD246 pKa = 3.71GLHH249 pKa = 4.74EE250 pKa = 4.63HH251 pKa = 7.03EE252 pKa = 4.22EE253 pKa = 4.31HH254 pKa = 6.76EE255 pKa = 4.8IEE257 pKa = 4.75VNN259 pKa = 3.27

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H8S961|A0A7H8S961_9BACI ATP synthase subunit b OS=Lentibacillus sp. CBA3610 OX=2518176 GN=atpF PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.66DD26 pKa = 3.03GRR28 pKa = 11.84QVLKK32 pKa = 10.25RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.96GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3575

0

3575

970708

26

2013

271.5

30.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.116 ± 0.042

0.634 ± 0.012

5.969 ± 0.046

7.582 ± 0.062

4.426 ± 0.043

6.903 ± 0.044

2.2 ± 0.022

7.579 ± 0.045

6.041 ± 0.043

9.282 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.023

4.686 ± 0.035

3.639 ± 0.025

3.931 ± 0.03

4.157 ± 0.03

5.935 ± 0.03

5.547 ± 0.029

6.79 ± 0.037

1.056 ± 0.018

3.536 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski