Lachnospiraceae bacterium XBD2001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6XDN4|A0A1I6XDN4_9FIRM Multimeric flavodoxin WrbA OS=Lachnospiraceae bacterium XBD2001 OX=1520820 GN=SAMN02910301_0694 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84TWLLVGMSVMLMLSVSACGTKK24 pKa = 9.62TEE26 pKa = 4.29NTATPQAPDD35 pKa = 3.53AEE37 pKa = 4.44PVIEE41 pKa = 4.45EE42 pKa = 4.31PATEE46 pKa = 4.01EE47 pKa = 4.26PEE49 pKa = 4.62AEE51 pKa = 4.16EE52 pKa = 5.0SDD54 pKa = 3.63TGLANPWTEE63 pKa = 3.74SDD65 pKa = 3.71RR66 pKa = 11.84EE67 pKa = 4.24GVLAATGFDD76 pKa = 4.01LNVPDD81 pKa = 3.71EE82 pKa = 4.78AEE84 pKa = 4.09NVVYY88 pKa = 10.5SYY90 pKa = 11.08MEE92 pKa = 4.59ADD94 pKa = 3.47KK95 pKa = 9.93MAQVSYY101 pKa = 11.44VMNNHH106 pKa = 6.53DD107 pKa = 2.88WTYY110 pKa = 10.9RR111 pKa = 11.84VQPTDD116 pKa = 3.23GLQDD120 pKa = 3.28ISGMYY125 pKa = 8.89YY126 pKa = 9.81EE127 pKa = 4.98WVAEE131 pKa = 3.91EE132 pKa = 4.16DD133 pKa = 4.01TNVANHH139 pKa = 5.51EE140 pKa = 4.33AEE142 pKa = 3.64IKK144 pKa = 10.57AYY146 pKa = 10.46VEE148 pKa = 4.36NNGDD152 pKa = 4.07PDD154 pKa = 3.9NLDD157 pKa = 3.9DD158 pKa = 3.99MFSVQVLNWYY168 pKa = 8.94DD169 pKa = 3.33ATEE172 pKa = 4.4GATHH176 pKa = 6.22SVSISGGAVDD186 pKa = 5.72GLDD189 pKa = 3.15LSVYY193 pKa = 10.73AEE195 pKa = 4.34DD196 pKa = 3.47MWMKK200 pKa = 10.92

Molecular weight: 22.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6YRQ9|A0A1I6YRQ9_9FIRM Indole-3-glycerol phosphate synthase OS=Lachnospiraceae bacterium XBD2001 OX=1520820 GN=trpC PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.62GGRR28 pKa = 11.84KK29 pKa = 8.94VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2215

0

2215

756667

37

3687

341.6

38.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.092 ± 0.057

1.35 ± 0.021

6.129 ± 0.041

7.552 ± 0.06

4.08 ± 0.04

7.113 ± 0.042

1.877 ± 0.023

7.035 ± 0.051

6.032 ± 0.05

8.766 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.029

4.363 ± 0.035

3.288 ± 0.025

3.161 ± 0.027

4.314 ± 0.041

5.624 ± 0.04

5.593 ± 0.069

7.356 ± 0.046

0.889 ± 0.017

4.216 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski