Legionella endosymbiont of Polyplax serrata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 8.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D3R7Q6|A0A2D3R7Q6_9GAMM Cytochrome c biogenesis ATP-binding export protein CcmA OS=Legionella endosymbiont of Polyplax serrata OX=2005262 GN=ccmA PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.11KK3 pKa = 9.07YY4 pKa = 10.53KK5 pKa = 10.42KK6 pKa = 9.62YY7 pKa = 10.14ICVICGFIYY16 pKa = 10.59DD17 pKa = 4.08EE18 pKa = 4.32EE19 pKa = 5.06KK20 pKa = 11.04GFPEE24 pKa = 5.41DD25 pKa = 4.06NINPGTLWEE34 pKa = 5.62DD35 pKa = 3.16IPEE38 pKa = 3.89NWLCPEE44 pKa = 4.44CGVTKK49 pKa = 10.61EE50 pKa = 4.79DD51 pKa = 4.33FEE53 pKa = 5.01QII55 pKa = 3.48

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D3R8V0|A0A2D3R8V0_9GAMM Phosphatidylglycerophosphate synthase OS=Legionella endosymbiont of Polyplax serrata OX=2005262 GN=CCU22_01245 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 7.95RR14 pKa = 11.84CHH16 pKa = 6.05GFRR19 pKa = 11.84CRR21 pKa = 11.84MSTKK25 pKa = 10.28NGRR28 pKa = 11.84LVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 9.85RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.41RR41 pKa = 11.84LSVV44 pKa = 3.08

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

473

0

473

149568

38

1403

316.2

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.503 ± 0.082

1.49 ± 0.037

4.141 ± 0.084

4.444 ± 0.097

5.971 ± 0.117

5.24 ± 0.106

1.805 ± 0.044

12.531 ± 0.127

10.393 ± 0.158

10.159 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.046

7.812 ± 0.107

2.89 ± 0.051

2.505 ± 0.056

3.511 ± 0.086

7.058 ± 0.073

3.99 ± 0.081

4.915 ± 0.118

0.891 ± 0.043

4.62 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski