Euphorbia heterophylla associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus euhet1

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345S7V1|A0A345S7V1_9VIRU Replication-associated protein OS=Euphorbia heterophylla associated gemycircularvirus OX=2291614 PE=3 SV=1
MM1 pKa = 7.64SFTTNGRR8 pKa = 11.84YY9 pKa = 9.13FLVTYY14 pKa = 7.77AQCGDD19 pKa = 4.01LDD21 pKa = 3.89PFRR24 pKa = 11.84VVDD27 pKa = 3.95KK28 pKa = 10.74FSSLGSEE35 pKa = 4.34CIIGRR40 pKa = 11.84EE41 pKa = 3.92LHH43 pKa = 6.64EE44 pKa = 5.48DD45 pKa = 3.55GGLHH49 pKa = 5.93LHH51 pKa = 6.61CFVDD55 pKa = 4.63FGRR58 pKa = 11.84KK59 pKa = 8.02FRR61 pKa = 11.84SRR63 pKa = 11.84KK64 pKa = 7.79TDD66 pKa = 2.98VFDD69 pKa = 3.81VDD71 pKa = 3.68GRR73 pKa = 11.84HH74 pKa = 6.13PNIEE78 pKa = 4.17SSRR81 pKa = 11.84GTPEE85 pKa = 3.23KK86 pKa = 10.53GYY88 pKa = 11.15NYY90 pKa = 9.99AIKK93 pKa = 10.82DD94 pKa = 3.54GDD96 pKa = 4.0VVAGGLQRR104 pKa = 11.84PEE106 pKa = 3.91PKK108 pKa = 10.31SRR110 pKa = 11.84DD111 pKa = 3.45GAGSTFEE118 pKa = 3.98KK119 pKa = 10.22WSLITSATDD128 pKa = 3.42RR129 pKa = 11.84EE130 pKa = 4.54EE131 pKa = 3.71FWRR134 pKa = 11.84LVHH137 pKa = 6.84EE138 pKa = 5.35LDD140 pKa = 4.34PKK142 pKa = 10.76SAACSFTQLSKK153 pKa = 11.32YY154 pKa = 10.58ADD156 pKa = 3.33SKK158 pKa = 9.27YY159 pKa = 11.04AEE161 pKa = 4.23VPTEE165 pKa = 3.99YY166 pKa = 9.9EE167 pKa = 3.92HH168 pKa = 7.4PGGIEE173 pKa = 3.7FAPGDD178 pKa = 4.39GRR180 pKa = 11.84PMSLVLYY187 pKa = 10.34GEE189 pKa = 4.26SRR191 pKa = 11.84TGKK194 pKa = 7.35TLWARR199 pKa = 11.84SLGRR203 pKa = 11.84HH204 pKa = 6.0IYY206 pKa = 10.36NVGLVSGAEE215 pKa = 4.06CVKK218 pKa = 10.88ASEE221 pKa = 3.66VDD223 pKa = 3.53YY224 pKa = 11.45AVFDD228 pKa = 5.08DD229 pKa = 4.23IRR231 pKa = 11.84GGMKK235 pKa = 9.7FFPAFKK241 pKa = 9.52EE242 pKa = 4.07WLGGQHH248 pKa = 5.3TVCVKK253 pKa = 10.12QLYY256 pKa = 9.69RR257 pKa = 11.84DD258 pKa = 4.33PILVKK263 pKa = 9.81WGKK266 pKa = 9.37PSIWVSNDD274 pKa = 3.04DD275 pKa = 4.09PRR277 pKa = 11.84LCMEE281 pKa = 5.06PSDD284 pKa = 4.56VAWLEE289 pKa = 3.74ANARR293 pKa = 11.84FIEE296 pKa = 4.52CNHH299 pKa = 6.77AIFRR303 pKa = 11.84ANTEE307 pKa = 3.84

Molecular weight: 34.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345S7V1|A0A345S7V1_9VIRU Replication-associated protein OS=Euphorbia heterophylla associated gemycircularvirus OX=2291614 PE=3 SV=1
MM1 pKa = 7.99PYY3 pKa = 9.67RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.07ARR8 pKa = 11.84KK9 pKa = 7.4PARR12 pKa = 11.84NRR14 pKa = 11.84RR15 pKa = 11.84YY16 pKa = 8.38GGVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84YY23 pKa = 9.07PIKK26 pKa = 9.51KK27 pKa = 6.92TQFRR31 pKa = 11.84KK32 pKa = 10.17RR33 pKa = 11.84RR34 pKa = 11.84MTPRR38 pKa = 11.84KK39 pKa = 9.48RR40 pKa = 11.84ILNIASRR47 pKa = 11.84KK48 pKa = 9.52KK49 pKa = 9.71RR50 pKa = 11.84DD51 pKa = 3.38NMIANTNITISPKK64 pKa = 10.15PGTFASGGAVLTGDD78 pKa = 3.62RR79 pKa = 11.84QYY81 pKa = 10.93IIPWVASGRR90 pKa = 11.84PLNGSDD96 pKa = 3.14NTNNTIAEE104 pKa = 4.2AAARR108 pKa = 11.84TATEE112 pKa = 4.21CYY114 pKa = 9.52MRR116 pKa = 11.84GLNEE120 pKa = 4.62KK121 pKa = 9.97IQIQTSTAQPWQWRR135 pKa = 11.84RR136 pKa = 11.84VCFQYY141 pKa = 10.42RR142 pKa = 11.84QRR144 pKa = 11.84DD145 pKa = 3.46ILNTQNADD153 pKa = 3.59TPLVAQTAAVGLTRR167 pKa = 11.84GLVDD171 pKa = 3.96ALQNGTMAQTLLNVLFQGRR190 pKa = 11.84QSIDD194 pKa = 2.63WASFFTAKK202 pKa = 10.01VDD204 pKa = 3.56NRR206 pKa = 11.84NVTLCYY212 pKa = 10.55DD213 pKa = 3.78RR214 pKa = 11.84TRR216 pKa = 11.84IINSGNNNGIIRR228 pKa = 11.84NYY230 pKa = 9.91KK231 pKa = 8.65IWHH234 pKa = 6.46PMNKK238 pKa = 7.31TLRR241 pKa = 11.84YY242 pKa = 10.16GDD244 pKa = 4.87DD245 pKa = 3.22EE246 pKa = 4.59TGNDD250 pKa = 3.29EE251 pKa = 4.34TSSFFCSASNYY262 pKa = 10.42GMGDD266 pKa = 3.54YY267 pKa = 10.78YY268 pKa = 10.49IVDD271 pKa = 3.91IFSAGPSGTSNDD283 pKa = 3.4QLKK286 pKa = 10.38FEE288 pKa = 5.03PEE290 pKa = 3.5ATLYY294 pKa = 8.42WHH296 pKa = 6.69EE297 pKa = 4.18RR298 pKa = 3.47

Molecular weight: 34.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

605

298

307

302.5

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.273 ± 0.527

1.983 ± 0.433

6.116 ± 0.731

4.959 ± 1.536

4.628 ± 0.859

8.264 ± 0.822

1.818 ± 0.775

5.289 ± 0.96

5.124 ± 0.288

6.116 ± 0.504

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.818 ± 0.358

5.455 ± 1.982

4.628 ± 0.179

3.306 ± 1.167

8.76 ± 1.336

6.446 ± 0.727

6.612 ± 1.427

5.289 ± 1.079

2.149 ± 0.091

3.967 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski