Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Rhodopseudomonas; Rhodopseudomonas palustris

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6NCK3|Q6NCK3_RHOPA Putative sulfate ester transporter ATP-binding component OS=Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) OX=258594 GN=AtsC PE=3 SV=1
MM1 pKa = 7.56SLTGALSSAISALNAQSQSLSMISDD26 pKa = 4.62NIANSDD32 pKa = 3.35TTGYY36 pKa = 8.18KK37 pKa = 7.24TTSAMFSQLVTASSSATSYY56 pKa = 11.42ASGGVTVAGRR66 pKa = 11.84ANISQQGLLAATSNATDD83 pKa = 3.37VAIQGSGFFVVSDD96 pKa = 3.61QTSGGTTSYY105 pKa = 9.71TRR107 pKa = 11.84NGAFSIDD114 pKa = 3.33NAGYY118 pKa = 10.01LVNSGYY124 pKa = 10.54YY125 pKa = 9.99LEE127 pKa = 4.73GWRR130 pKa = 11.84TDD132 pKa = 3.12ADD134 pKa = 3.74GKK136 pKa = 10.75IVGDD140 pKa = 3.81ASSATLSPINTNVAATSGSATTKK163 pKa = 8.07TTFAANLPADD173 pKa = 3.99AATGATYY180 pKa = 10.48SSSMTVYY187 pKa = 10.96DD188 pKa = 4.16SLGTANSIQVTWIKK202 pKa = 9.24TASNAWTASFANPTLSSSTSTTTGTTSGSVSLTFNNDD239 pKa = 2.11GSLASTNPSPPTISVSGWTNGAANSTISLDD269 pKa = 3.37LGTVGKK275 pKa = 9.0TNGLTQYY282 pKa = 11.24ASGEE286 pKa = 4.06ATPTINVTSISSDD299 pKa = 2.95GLPYY303 pKa = 10.93GKK305 pKa = 10.14LSSIAVGDD313 pKa = 4.25DD314 pKa = 3.26GTVNATYY321 pKa = 11.24SNGQTIAIYY330 pKa = 9.9KK331 pKa = 9.49IAVATFKK338 pKa = 11.24DD339 pKa = 3.8PDD341 pKa = 3.9GLSVNSYY348 pKa = 10.87GIYY351 pKa = 10.33SVTAASGDD359 pKa = 3.57ASVQTSGSNGAGKK372 pKa = 10.15IYY374 pKa = 10.31GSEE377 pKa = 4.4LEE379 pKa = 4.48SSTTDD384 pKa = 2.93TSSQFASMISAQQAYY399 pKa = 9.56SAASQVITTVNKK411 pKa = 9.81MFDD414 pKa = 3.61TLISAMRR421 pKa = 3.98

Molecular weight: 42.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6NC61|Q6NC61_RHOPA Cupin_5 domain-containing protein OS=Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) OX=258594 GN=RPA0611 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATTGGRR28 pKa = 11.84KK29 pKa = 9.06VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4811

0

4811

1579866

25

4335

328.4

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.807 ± 0.046

0.85 ± 0.012

5.65 ± 0.023

5.218 ± 0.033

3.641 ± 0.021

8.366 ± 0.037

1.976 ± 0.018

5.263 ± 0.025

3.514 ± 0.027

10.06 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.018

2.581 ± 0.02

5.393 ± 0.029

3.282 ± 0.02

7.002 ± 0.033

5.59 ± 0.025

5.465 ± 0.025

7.495 ± 0.029

1.28 ± 0.014

2.224 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski