bacterium M21

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202E1G1|A0A202E1G1_9BACT Uncharacterized protein OS=bacterium M21 OX=1932697 GN=BVY04_02535 PE=4 SV=1
MM1 pKa = 6.91STFVRR6 pKa = 11.84IPRR9 pKa = 11.84VILPILMTICIGNIGAQNTPTRR31 pKa = 11.84INLSSTGQEE40 pKa = 3.91ANSWSYY46 pKa = 10.85YY47 pKa = 9.75PKK49 pKa = 10.61LSTDD53 pKa = 2.6GRR55 pKa = 11.84YY56 pKa = 8.8VVYY59 pKa = 10.34EE60 pKa = 4.02SSASNLVDD68 pKa = 4.1FDD70 pKa = 5.04YY71 pKa = 11.77NGVTDD76 pKa = 4.59IFVHH80 pKa = 6.72DD81 pKa = 4.75LQTGATEE88 pKa = 4.14RR89 pKa = 11.84VSVSSTGQEE98 pKa = 3.74ADD100 pKa = 3.81RR101 pKa = 11.84DD102 pKa = 4.34CYY104 pKa = 11.1SPNISSDD111 pKa = 2.44GRR113 pKa = 11.84YY114 pKa = 8.95VVFTSQATNLTWGDD128 pKa = 3.68GNTTAVYY135 pKa = 10.1IHH137 pKa = 7.32DD138 pKa = 4.78RR139 pKa = 11.84DD140 pKa = 3.64LDD142 pKa = 3.67ITEE145 pKa = 4.4SVYY148 pKa = 10.84TPMSGEE154 pKa = 4.0YY155 pKa = 9.39TNNPSVSTDD164 pKa = 3.1GRR166 pKa = 11.84WISFSSMSSYY176 pKa = 11.3VVGNDD181 pKa = 3.11SNNAEE186 pKa = 4.99DD187 pKa = 4.14IFLIDD192 pKa = 3.38RR193 pKa = 11.84QTYY196 pKa = 10.68NITCVSATPSGFTGNSGSYY215 pKa = 9.35DD216 pKa = 3.38AKK218 pKa = 9.52ITPDD222 pKa = 2.91ASYY225 pKa = 11.77VLFGSGASDD234 pKa = 6.08LIDD237 pKa = 3.42NDD239 pKa = 3.81YY240 pKa = 11.63NNRR243 pKa = 11.84NDD245 pKa = 3.49LFLYY249 pKa = 9.38TVATSSLEE257 pKa = 4.17RR258 pKa = 11.84ISISDD263 pKa = 4.35DD264 pKa = 3.12EE265 pKa = 5.27SEE267 pKa = 5.34ANDD270 pKa = 3.32STYY273 pKa = 11.28YY274 pKa = 10.83GAITADD280 pKa = 3.09ARR282 pKa = 11.84YY283 pKa = 9.74VAFSSNATNLTMGSDD298 pKa = 3.65FNGYY302 pKa = 9.73EE303 pKa = 4.58DD304 pKa = 3.18IFLRR308 pKa = 11.84DD309 pKa = 4.16RR310 pKa = 11.84ILGTTVRR317 pKa = 11.84LSNGYY322 pKa = 9.74DD323 pKa = 3.09GSGANSYY330 pKa = 10.86SSNPSITDD338 pKa = 3.43DD339 pKa = 3.66GQTVVFSSSANNLVATDD356 pKa = 3.91TNTDD360 pKa = 3.01ADD362 pKa = 4.29LFIVDD367 pKa = 3.95VATGATEE374 pKa = 4.9IITLGYY380 pKa = 10.22DD381 pKa = 3.5GSSANSYY388 pKa = 10.41SYY390 pKa = 9.81WGYY393 pKa = 10.5ISAAGTAVGIEE404 pKa = 4.12SSASNLVDD412 pKa = 3.27NDD414 pKa = 3.51FNGYY418 pKa = 10.22SDD420 pKa = 3.35IFVIEE425 pKa = 4.21LSDD428 pKa = 3.82IGSLPATIVYY438 pKa = 9.84VDD440 pKa = 4.74PAAQGTGNDD449 pKa = 3.52GTSWYY454 pKa = 10.07DD455 pKa = 3.2AYY457 pKa = 11.69LNLQDD462 pKa = 5.22ALLSEE467 pKa = 4.69LQPNTEE473 pKa = 3.58IWVAAGTYY481 pKa = 9.07KK482 pKa = 10.88ASDD485 pKa = 3.27TGDD488 pKa = 3.4RR489 pKa = 11.84QASFHH494 pKa = 5.97LASRR498 pKa = 11.84VSILGGFNGTEE509 pKa = 3.71IEE511 pKa = 4.37KK512 pKa = 10.49DD513 pKa = 3.31QRR515 pKa = 11.84DD516 pKa = 3.79PKK518 pKa = 10.93LNPTILSGDD527 pKa = 4.17LNSDD531 pKa = 3.51DD532 pKa = 4.16TPGFLNRR539 pKa = 11.84SDD541 pKa = 3.63NSYY544 pKa = 10.45HH545 pKa = 6.01VVYY548 pKa = 10.29AANAYY553 pKa = 10.55SSILDD558 pKa = 3.53GLTITGGFANGASPDD573 pKa = 3.6EE574 pKa = 4.54RR575 pKa = 11.84GGGVFSMDD583 pKa = 2.98STLLIDD589 pKa = 3.58QCSIKK594 pKa = 11.0NNFAIQEE601 pKa = 4.14GGALYY606 pKa = 10.51LDD608 pKa = 4.26RR609 pKa = 11.84VATTITSTAFSGNRR623 pKa = 11.84AVYY626 pKa = 9.99GGAISFMDD634 pKa = 4.62GPSTISLPTITSCTFTSNYY653 pKa = 8.77SDD655 pKa = 3.89YY656 pKa = 10.52YY657 pKa = 8.85GTVRR661 pKa = 11.84CMYY664 pKa = 8.95TSPTITNCLFTKK676 pKa = 10.22NYY678 pKa = 9.68CGYY681 pKa = 8.67DD682 pKa = 3.52TAVLASVGGGNNEE695 pKa = 3.22IGYY698 pKa = 8.11ITNCTFTDD706 pKa = 3.56NSSGSPSAEE715 pKa = 3.54TVLAGTSFVIKK726 pKa = 10.78NSIFWNNTSTEE737 pKa = 4.19VYY739 pKa = 9.86IAASPFGTIVEE750 pKa = 4.4SSCLKK755 pKa = 10.74DD756 pKa = 3.61GLIGTGNIDD765 pKa = 3.76AAPLFIDD772 pKa = 4.89PATDD776 pKa = 3.6DD777 pKa = 3.32YY778 pKa = 11.22RR779 pKa = 11.84LNVSSPCVNTGLLEE793 pKa = 4.19TGIPTTDD800 pKa = 3.27LNGAARR806 pKa = 11.84DD807 pKa = 3.79AFPDD811 pKa = 3.55MGCYY815 pKa = 9.73EE816 pKa = 4.47YY817 pKa = 11.67VNPTDD822 pKa = 3.38LTIRR826 pKa = 11.84VYY828 pKa = 11.02ASQDD832 pKa = 3.27GNVKK836 pKa = 10.49LGTNGIPSTYY846 pKa = 9.85VSEE849 pKa = 4.43SVSTGGASSEE859 pKa = 4.65FYY861 pKa = 10.8AVPNSGYY868 pKa = 10.2QFSQWSDD875 pKa = 2.57GDD877 pKa = 4.03TNNPRR882 pKa = 11.84TFVSVSSDD890 pKa = 2.63ITVTAEE896 pKa = 3.63FTVASTFQVTANAGEE911 pKa = 4.16NGSVKK916 pKa = 9.95LTDD919 pKa = 3.39TGATQPSVSDD929 pKa = 3.6YY930 pKa = 11.57VFGGDD935 pKa = 3.39SSQEE939 pKa = 3.74FFALANTGYY948 pKa = 10.83LFSQWSDD955 pKa = 2.95GNTDD959 pKa = 3.19NPRR962 pKa = 11.84IFTNVTADD970 pKa = 3.38TMVVAEE976 pKa = 5.22FINNTFDD983 pKa = 3.15VTANAGPDD991 pKa = 3.38GMVQLTTVGTPLHH1004 pKa = 6.45SVTDD1008 pKa = 4.06SVFGGSDD1015 pKa = 2.96SSEE1018 pKa = 3.86FFALANTGYY1027 pKa = 10.83LFSQWSDD1034 pKa = 2.95GNTDD1038 pKa = 3.19NPRR1041 pKa = 11.84IFTNVTADD1049 pKa = 3.49LMVVAEE1055 pKa = 5.08FINNTFDD1062 pKa = 3.55VIANAGPDD1070 pKa = 3.36GMVRR1074 pKa = 11.84LTTVGTPLPSVTDD1087 pKa = 3.79SVFGGSDD1094 pKa = 2.96SSEE1097 pKa = 3.86FFALANTGYY1106 pKa = 10.83LFSQWSDD1113 pKa = 2.91GNTEE1117 pKa = 3.87NPRR1120 pKa = 11.84IFTNVTADD1128 pKa = 3.49LMVVAEE1134 pKa = 5.08FINNTFDD1141 pKa = 3.14VTANAGSDD1149 pKa = 3.45GMVQLTTVGAPLPTVTDD1166 pKa = 4.36SIIGGSDD1173 pKa = 2.82SSEE1176 pKa = 3.78FFALANTGYY1185 pKa = 10.83LFSQWSDD1192 pKa = 2.95GNTDD1196 pKa = 3.19NPRR1199 pKa = 11.84IFTNVTADD1207 pKa = 3.49LMIVAEE1213 pKa = 4.87FIKK1216 pKa = 10.79QNEE1219 pKa = 4.1PPIAVAEE1226 pKa = 4.08NFDD1229 pKa = 3.91LSTIIGDD1236 pKa = 4.53TITLTGSNSHH1246 pKa = 7.39DD1247 pKa = 4.03PDD1249 pKa = 3.27TWPVVPITFSWNYY1262 pKa = 9.67VSGPEE1267 pKa = 4.04AMPGPVLTDD1276 pKa = 3.17NTGDD1280 pKa = 3.49LMEE1283 pKa = 4.37VTFVPTAVGSYY1294 pKa = 9.15VFEE1297 pKa = 4.28LTVDD1301 pKa = 4.04DD1302 pKa = 5.31GADD1305 pKa = 3.02THH1307 pKa = 5.74TTQIVINVVGIPEE1320 pKa = 4.05IDD1322 pKa = 3.41VTGDD1326 pKa = 2.95MDD1328 pKa = 4.31FGSIAGASGTAAKK1341 pKa = 9.47TYY1343 pKa = 9.35TISNPGTGALTLTGIPVIEE1362 pKa = 4.26ITGDD1366 pKa = 3.08AAADD1370 pKa = 3.82FTVTAAPATSIAAGGQTTFEE1390 pKa = 4.36VTFDD1394 pKa = 3.68PSAVGVRR1401 pKa = 11.84SATISIANDD1410 pKa = 3.57DD1411 pKa = 4.18ADD1413 pKa = 3.93EE1414 pKa = 4.34NPYY1417 pKa = 10.51VIAVQGTGTNAAPLASATAPVSAVVGDD1444 pKa = 3.86TVNLSGSASSDD1455 pKa = 3.1ADD1457 pKa = 3.48SWPSALTYY1465 pKa = 10.21SWGYY1469 pKa = 10.49VSGPDD1474 pKa = 4.32EE1475 pKa = 4.2PALTNGATAEE1485 pKa = 4.23SSFVPTVHH1493 pKa = 5.74GTYY1496 pKa = 9.67TYY1498 pKa = 10.74EE1499 pKa = 3.88LTVNDD1504 pKa = 3.89SAATATAQVMINVRR1518 pKa = 11.84AALPRR1523 pKa = 11.84LTTGVASVSSTAWYY1537 pKa = 8.52TVDD1540 pKa = 5.24LPYY1543 pKa = 10.36TYY1545 pKa = 11.18DD1546 pKa = 3.43SMVVVCTPVNDD1557 pKa = 4.27SNIGPLAVRR1566 pKa = 11.84VKK1568 pKa = 9.64GTTGSSFQIQLTHH1581 pKa = 6.74SSTDD1585 pKa = 3.36TTPQALMVHH1594 pKa = 5.5YY1595 pKa = 9.27MVIEE1599 pKa = 4.2EE1600 pKa = 4.34GNYY1603 pKa = 8.51TVAEE1607 pKa = 4.31HH1608 pKa = 6.78GIKK1611 pKa = 9.43MEE1613 pKa = 4.01AVKK1616 pKa = 10.91YY1617 pKa = 10.55SSSQFAGKK1625 pKa = 10.0GRR1627 pKa = 11.84WGTVDD1632 pKa = 3.06AQSYY1636 pKa = 9.27INSYY1640 pKa = 10.03SNPVVVGQVQTANDD1654 pKa = 3.32MRR1656 pKa = 11.84YY1657 pKa = 8.37QAFWARR1663 pKa = 11.84GASQSASPSATVLNVGRR1680 pKa = 11.84HH1681 pKa = 4.94IGEE1684 pKa = 4.79DD1685 pKa = 3.37LDD1687 pKa = 4.31KK1688 pKa = 11.41VRR1690 pKa = 11.84LDD1692 pKa = 3.66EE1693 pKa = 4.17EE1694 pKa = 3.87VGYY1697 pKa = 10.84VVFEE1701 pKa = 4.28SGNAIANGISLVAGIGSDD1719 pKa = 3.88SIKK1722 pKa = 10.99GVDD1725 pKa = 3.31NGSYY1729 pKa = 10.33SYY1731 pKa = 10.76AISGLTSPDD1740 pKa = 3.27TAILAASGMDD1750 pKa = 3.38GGDD1753 pKa = 2.98GGFPILTGTTPVTASAIALTFEE1775 pKa = 4.17EE1776 pKa = 5.62DD1777 pKa = 3.6VLKK1780 pKa = 10.1DD1781 pKa = 3.36TEE1783 pKa = 4.3RR1784 pKa = 11.84KK1785 pKa = 7.88HH1786 pKa = 5.03TAEE1789 pKa = 3.81SVAYY1793 pKa = 10.15VVFDD1797 pKa = 3.72TPP1799 pKa = 4.13

Molecular weight: 189.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202E047|A0A202E047_9BACT CMD domain-containing protein OS=bacterium M21 OX=1932697 GN=BVY04_02975 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.23SSICAACGKK12 pKa = 9.56PFVRR16 pKa = 11.84CRR18 pKa = 11.84YY19 pKa = 9.43NSNHH23 pKa = 5.57QKK25 pKa = 9.39FCRR28 pKa = 11.84RR29 pKa = 11.84SACVRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.39QARR40 pKa = 11.84QRR42 pKa = 11.84TSHH45 pKa = 5.13NRR47 pKa = 11.84RR48 pKa = 11.84YY49 pKa = 10.34HH50 pKa = 5.62EE51 pKa = 4.74DD52 pKa = 3.15EE53 pKa = 5.12DD54 pKa = 3.8YY55 pKa = 11.52RR56 pKa = 11.84EE57 pKa = 4.4GKK59 pKa = 8.63RR60 pKa = 11.84QKK62 pKa = 9.49SRR64 pKa = 11.84EE65 pKa = 3.83YY66 pKa = 9.15MRR68 pKa = 11.84VRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84KK73 pKa = 9.08EE74 pKa = 3.17RR75 pKa = 11.84AAKK78 pKa = 9.95KK79 pKa = 10.12DD80 pKa = 3.72AIEE83 pKa = 4.45INPIDD88 pKa = 3.87ILTGVVAQLTDD99 pKa = 3.61EE100 pKa = 4.58EE101 pKa = 5.38DD102 pKa = 3.42PMTVRR107 pKa = 11.84EE108 pKa = 4.06RR109 pKa = 11.84LRR111 pKa = 11.84AYY113 pKa = 9.79SARR116 pKa = 11.84GRR118 pKa = 11.84QLSHH122 pKa = 6.41ICSITGPVPP131 pKa = 3.22

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1049

0

1049

336607

42

2755

320.9

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.412 ± 0.071

1.365 ± 0.031

5.914 ± 0.063

6.694 ± 0.078

3.965 ± 0.049

7.199 ± 0.093

2.206 ± 0.036

6.088 ± 0.053

5.642 ± 0.099

9.787 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.035

3.79 ± 0.05

4.258 ± 0.049

3.752 ± 0.057

5.57 ± 0.083

6.044 ± 0.076

5.788 ± 0.099

6.719 ± 0.07

1.267 ± 0.027

2.961 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski