Roseimicrobium gellanilyticum

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; Roseimicrobium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A366HRP9|A0A366HRP9_9BACT Uncharacterized protein OS=Roseimicrobium gellanilyticum OX=748857 GN=DES53_102313 PE=4 SV=1
MM1 pKa = 7.46QILAVAAMSFSPLPSGMASWSDD23 pKa = 3.1SDD25 pKa = 4.96FNGSNDD31 pKa = 3.14TWSDD35 pKa = 3.47PLTSQVTTLADD46 pKa = 4.44LNLQNNDD53 pKa = 3.64ADD55 pKa = 4.5NDD57 pKa = 4.04GATNDD62 pKa = 3.79EE63 pKa = 4.39EE64 pKa = 4.98ASEE67 pKa = 4.61GSDD70 pKa = 3.0PFSFDD75 pKa = 2.63TDD77 pKa = 3.62YY78 pKa = 11.87DD79 pKa = 3.9GLSDD83 pKa = 4.11GDD85 pKa = 3.92EE86 pKa = 4.05LHH88 pKa = 6.71LVKK91 pKa = 10.63PSLAVSLTDD100 pKa = 3.16WDD102 pKa = 3.89TDD104 pKa = 3.27NDD106 pKa = 3.89EE107 pKa = 4.38VSDD110 pKa = 3.81YY111 pKa = 11.65DD112 pKa = 3.32EE113 pKa = 3.87WHH115 pKa = 6.3NFSGVTYY122 pKa = 10.13PGGQLPSYY130 pKa = 9.69PGASYY135 pKa = 11.25SDD137 pKa = 3.77YY138 pKa = 11.51DD139 pKa = 3.72GDD141 pKa = 4.11GFKK144 pKa = 11.17NPVDD148 pKa = 3.88PQPTVPYY155 pKa = 10.5DD156 pKa = 3.41PTTTDD161 pKa = 2.87SDD163 pKa = 3.71SDD165 pKa = 3.46NWYY168 pKa = 10.03DD169 pKa = 4.82AYY171 pKa = 11.03DD172 pKa = 4.32PFPNDD177 pKa = 3.37PTNFSPINGIAWGQTFHH194 pKa = 7.96DD195 pKa = 4.86DD196 pKa = 3.94QDD198 pKa = 4.56GDD200 pKa = 5.03LLLNWEE206 pKa = 4.77DD207 pKa = 3.68PVPYY211 pKa = 10.16GPSDD215 pKa = 3.63SDD217 pKa = 3.71SDD219 pKa = 4.5GITDD223 pKa = 4.73GEE225 pKa = 4.5DD226 pKa = 3.65PYY228 pKa = 11.24PIDD231 pKa = 4.55PYY233 pKa = 11.27NYY235 pKa = 9.43SQINHH240 pKa = 6.77IYY242 pKa = 7.94WHH244 pKa = 6.43GEE246 pKa = 3.56VRR248 pKa = 11.84GDD250 pKa = 3.58SDD252 pKa = 5.41NDD254 pKa = 3.53GTLNWEE260 pKa = 4.49DD261 pKa = 3.9PQPYY265 pKa = 9.7GPPDD269 pKa = 3.75SDD271 pKa = 3.51SDD273 pKa = 4.43GIIDD277 pKa = 4.84EE278 pKa = 4.43EE279 pKa = 4.65DD280 pKa = 4.54PYY282 pKa = 11.36PSDD285 pKa = 3.55PNNYY289 pKa = 9.34SEE291 pKa = 4.31TNYY294 pKa = 8.33VHH296 pKa = 7.38WYY298 pKa = 10.67AEE300 pKa = 4.05VLGDD304 pKa = 4.32SDD306 pKa = 5.99NDD308 pKa = 3.95GTPNWQDD315 pKa = 3.39PQPYY319 pKa = 9.73GPPANDD325 pKa = 3.4ADD327 pKa = 4.27ADD329 pKa = 4.03GFANDD334 pKa = 3.86IDD336 pKa = 4.29PFAFDD341 pKa = 3.79ASNYY345 pKa = 9.74SAINNTNWYY354 pKa = 10.19ADD356 pKa = 3.48VLGDD360 pKa = 4.1IDD362 pKa = 5.8SDD364 pKa = 4.89QILNWTDD371 pKa = 3.47PFPNDD376 pKa = 3.13STNYY380 pKa = 9.73SAINSTSWHH389 pKa = 6.53ADD391 pKa = 3.13VLGDD395 pKa = 4.38ADD397 pKa = 5.6SDD399 pKa = 4.74TIQNWQDD406 pKa = 3.02GAPYY410 pKa = 10.59GDD412 pKa = 4.44TPPPPDD418 pKa = 5.05PDD420 pKa = 3.7SDD422 pKa = 4.14GDD424 pKa = 4.0GLTEE428 pKa = 4.13STEE431 pKa = 4.05AAYY434 pKa = 8.15GTSDD438 pKa = 4.68SDD440 pKa = 4.37VDD442 pKa = 4.43CDD444 pKa = 5.24DD445 pKa = 6.25DD446 pKa = 4.94GLTDD450 pKa = 3.65HH451 pKa = 7.31EE452 pKa = 4.79EE453 pKa = 3.76LVIYY457 pKa = 7.3YY458 pKa = 7.33TNPLNMYY465 pKa = 9.56HH466 pKa = 7.55LSQTAGWGDD475 pKa = 4.84LYY477 pKa = 10.9TDD479 pKa = 3.85YY480 pKa = 11.8QMVDD484 pKa = 3.1PTDD487 pKa = 3.8FDD489 pKa = 4.56GDD491 pKa = 4.49SIPDD495 pKa = 4.15RR496 pKa = 11.84IEE498 pKa = 3.42LHH500 pKa = 6.53YY501 pKa = 11.47GMLPNDD507 pKa = 4.02AADD510 pKa = 3.6AAGDD514 pKa = 3.85LDD516 pKa = 4.68GNGISNSAQYY526 pKa = 10.96AAGRR530 pKa = 11.84SLLADD535 pKa = 3.07ILVYY539 pKa = 10.97DD540 pKa = 4.24GDD542 pKa = 4.49GDD544 pKa = 3.91GMTDD548 pKa = 3.14VFEE551 pKa = 6.09DD552 pKa = 3.29AHH554 pKa = 6.03QLNKK558 pKa = 10.82NYY560 pKa = 10.39FGDD563 pKa = 3.73AMADD567 pKa = 3.22EE568 pKa = 5.41DD569 pKa = 4.11MDD571 pKa = 4.07GVFNFEE577 pKa = 3.95EE578 pKa = 4.96SKK580 pKa = 10.97LQLNPRR586 pKa = 11.84IPDD589 pKa = 3.17THH591 pKa = 7.3ARR593 pKa = 11.84GGLGDD598 pKa = 4.69LGVLMEE604 pKa = 4.61AMLYY608 pKa = 10.13PEE610 pKa = 5.51GGAPTVDD617 pKa = 3.77GDD619 pKa = 4.72SDD621 pKa = 5.7GIPDD625 pKa = 3.64WQEE628 pKa = 3.54VSLTAASPVFTRR640 pKa = 11.84LAPNDD645 pKa = 3.54MDD647 pKa = 4.94GDD649 pKa = 4.33SMPDD653 pKa = 2.9IWEE656 pKa = 4.09HH657 pKa = 6.17QYY659 pKa = 11.27GRR661 pKa = 11.84WKK663 pKa = 10.9YY664 pKa = 7.51PTNGLYY670 pKa = 10.02VRR672 pKa = 11.84HH673 pKa = 6.74HH674 pKa = 7.36DD675 pKa = 3.49GAGDD679 pKa = 3.62IEE681 pKa = 5.63GDD683 pKa = 3.62GLSNYY688 pKa = 9.33HH689 pKa = 6.66EE690 pKa = 4.5YY691 pKa = 10.66WLMCDD696 pKa = 3.97PMINDD701 pKa = 4.53GGADD705 pKa = 3.72PDD707 pKa = 4.3GDD709 pKa = 3.94GLGYY713 pKa = 10.44AAEE716 pKa = 4.44LALGTHH722 pKa = 6.7LWVADD727 pKa = 3.41TDD729 pKa = 3.57MDD731 pKa = 4.95GISDD735 pKa = 4.05GEE737 pKa = 4.01EE738 pKa = 4.31DD739 pKa = 3.98FDD741 pKa = 6.74GDD743 pKa = 3.77GLSNITEE750 pKa = 4.2RR751 pKa = 11.84SLSTNALNKK760 pKa = 9.83DD761 pKa = 3.14TDD763 pKa = 3.79ADD765 pKa = 3.54GWDD768 pKa = 3.99DD769 pKa = 5.57DD770 pKa = 4.89EE771 pKa = 4.48EE772 pKa = 4.4QAMGSNPLSPEE783 pKa = 4.04DD784 pKa = 3.86QPILEE789 pKa = 4.62PMSSYY794 pKa = 11.39VSIQTRR800 pKa = 11.84WVHH803 pKa = 6.58SSWQEE808 pKa = 3.65HH809 pKa = 5.16GGEE812 pKa = 4.25YY813 pKa = 10.17YY814 pKa = 10.48SRR816 pKa = 11.84YY817 pKa = 9.12WYY819 pKa = 6.92WTWDD823 pKa = 3.58SVDD826 pKa = 3.57PPMGHH831 pKa = 6.61WEE833 pKa = 3.99EE834 pKa = 4.28QFYY837 pKa = 9.81STTTPRR843 pKa = 11.84DD844 pKa = 3.08EE845 pKa = 5.01AGQANFSDD853 pKa = 4.55GTSQPISTFSAAQGALGQVSQMDD876 pKa = 3.46WQEE879 pKa = 3.83SGEE882 pKa = 4.18RR883 pKa = 11.84SVSSSWTEE891 pKa = 3.33ITGRR895 pKa = 11.84PMPEE899 pKa = 3.58PPQYY903 pKa = 10.74FFYY906 pKa = 10.38PDD908 pKa = 3.46GGEE911 pKa = 4.25EE912 pKa = 4.22SPGWSGYY919 pKa = 8.57TEE921 pKa = 4.72DD922 pKa = 3.59NRR924 pKa = 11.84VWSGGSLEE932 pKa = 5.49VRR934 pKa = 11.84LAWKK938 pKa = 9.86PNAPKK943 pKa = 9.71PHH945 pKa = 6.7RR946 pKa = 11.84RR947 pKa = 11.84HH948 pKa = 6.48DD949 pKa = 3.61LTVTFMEE956 pKa = 4.5TEE958 pKa = 4.41TVNEE962 pKa = 4.3AEE964 pKa = 4.23PDD966 pKa = 3.44ITFHH970 pKa = 6.89ALKK973 pKa = 10.36ILANQQFSSAGGTNGGAINLVANASTPEE1001 pKa = 4.09TTVVKK1006 pKa = 10.69SLVQNAEE1013 pKa = 3.19IRR1015 pKa = 11.84TYY1017 pKa = 10.95DD1018 pKa = 4.11YY1019 pKa = 10.69YY1020 pKa = 10.65WKK1022 pKa = 10.35NQGSATNPRR1031 pKa = 11.84PDD1033 pKa = 3.71DD1034 pKa = 3.59NVRR1037 pKa = 11.84THH1039 pKa = 6.53YY1040 pKa = 10.0PDD1042 pKa = 3.41SSSPTGYY1049 pKa = 8.37RR1050 pKa = 11.84TKK1052 pKa = 10.15KK1053 pKa = 8.36TDD1055 pKa = 3.27KK1056 pKa = 10.99LKK1058 pKa = 10.06IAQWYY1063 pKa = 9.27DD1064 pKa = 3.31VVNGGVFHH1072 pKa = 7.04PNKK1075 pKa = 10.16IEE1077 pKa = 4.4ADD1079 pKa = 3.13TDD1081 pKa = 3.6AFFIRR1086 pKa = 11.84LTNPPQLPSGTTYY1099 pKa = 10.34RR1100 pKa = 11.84AKK1102 pKa = 10.48VWTEE1106 pKa = 3.62NASGAVVDD1114 pKa = 4.5SGADD1118 pKa = 3.36VEE1120 pKa = 4.56LQPVANQPGTYY1131 pKa = 8.93QSKK1134 pKa = 10.17GLCLVSNSYY1143 pKa = 11.59DD1144 pKa = 3.47DD1145 pKa = 4.47AQATVSGPDD1154 pKa = 3.18NGLNDD1159 pKa = 3.33QSYY1162 pKa = 8.95NAEE1165 pKa = 4.06LGGSVKK1171 pKa = 9.96IRR1173 pKa = 11.84FLFGTNPTATTVDD1186 pKa = 3.38MVLPVPVKK1194 pKa = 9.92KK1195 pKa = 9.0TLTVQGYY1202 pKa = 8.04ILKK1205 pKa = 10.31SGTISPSDD1213 pKa = 3.84VIAPAAANTYY1223 pKa = 10.79LEE1225 pKa = 4.21LAKK1228 pKa = 10.6EE1229 pKa = 4.08RR1230 pKa = 11.84LAQVGVEE1237 pKa = 3.95LDD1239 pKa = 3.74GTISTVTANPSGVSFGGFFPAKK1261 pKa = 10.09FEE1263 pKa = 4.27EE1264 pKa = 4.27PHH1266 pKa = 6.22GASSLTIPPEE1276 pKa = 4.05SVALFTALNPQVDD1289 pKa = 3.85VTFYY1293 pKa = 10.73FVSDD1297 pKa = 3.96FANSSNQGMTVVPKK1311 pKa = 8.55TTSLTAFHH1319 pKa = 6.52NRR1321 pKa = 11.84ILIDD1325 pKa = 3.82VPAMADD1331 pKa = 3.09DD1332 pKa = 4.02TLAHH1336 pKa = 6.47EE1337 pKa = 4.62FLHH1340 pKa = 7.42VLMNAVHH1347 pKa = 6.95GPPFNDD1353 pKa = 3.92FLRR1356 pKa = 11.84EE1357 pKa = 4.06HH1358 pKa = 6.9GSDD1361 pKa = 5.37DD1362 pKa = 3.88NNLWHH1367 pKa = 7.15HH1368 pKa = 6.7NDD1370 pKa = 3.45GAIGDD1375 pKa = 3.77IFKK1378 pKa = 9.64RR1379 pKa = 11.84TRR1381 pKa = 11.84INDD1384 pKa = 2.86AMYY1387 pKa = 10.23YY1388 pKa = 10.61KK1389 pKa = 10.07MYY1391 pKa = 7.55PTPPP1395 pKa = 3.43

Molecular weight:
152.72 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A366H6Z0|A0A366H6Z0_9BACT Uncharacterized protein OS=Roseimicrobium gellanilyticum OX=748857 GN=DES53_1143 PE=4 SV=1
MM1 pKa = 7.18NAIVYY6 pKa = 10.0VNTPLEE12 pKa = 4.49APEE15 pKa = 4.2HH16 pKa = 5.86AALAALARR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 4.0GRR28 pKa = 11.84AKK30 pKa = 10.38GQQLRR35 pKa = 11.84LLAIQAMRR43 pKa = 11.84DD44 pKa = 3.48RR45 pKa = 11.84NQGRR49 pKa = 11.84SKK51 pKa = 10.61SRR53 pKa = 11.84SRR55 pKa = 11.84GIMPAGSDD63 pKa = 3.56SYY65 pKa = 11.44VPHH68 pKa = 7.19APAPLPSCSCSSSPCSCSCSDD89 pKa = 3.82AGAAGSPKK97 pKa = 10.12VQLATASDD105 pKa = 3.37SRR107 pKa = 11.84TRR109 pKa = 11.84RR110 pKa = 11.84STSTNTNTNTSPCTGTGTDD129 pKa = 4.55ARR131 pKa = 11.84AALQKK136 pKa = 10.56GGRR139 pKa = 11.84ARR141 pKa = 11.84GG142 pKa = 3.38

Molecular weight:
14.64 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6509

0

6509

2350827

29

10978

361.2

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.239 ± 0.034

0.993 ± 0.012

5.251 ± 0.029

6.183 ± 0.047

3.786 ± 0.018

8.124 ± 0.071

2.377 ± 0.018

4.499 ± 0.023

4.689 ± 0.043

9.908 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.02

3.142 ± 0.05

5.521 ± 0.037

3.527 ± 0.021

6.124 ± 0.041

6.06 ± 0.03

5.886 ± 0.067

7.155 ± 0.029

1.666 ± 0.018

2.447 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski