Aeromonas phage ZPAH7B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Aerosvirus; Aeromonas virus ZPAH7

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B6L0|A0A411B6L0_9CAUD Uncharacterized protein OS=Aeromonas phage ZPAH7B OX=2501745 GN=ZPAH7B_orf00018 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 10.72FNVGSMMVMAGAALGALVSDD23 pKa = 4.39GALRR27 pKa = 11.84QVGVIRR33 pKa = 11.84TQYY36 pKa = 10.65TEE38 pKa = 3.39VDD40 pKa = 3.11INGEE44 pKa = 4.13RR45 pKa = 11.84KK46 pKa = 8.13VTGHH50 pKa = 6.34SIEE53 pKa = 4.81AVLEE57 pKa = 3.93CLAADD62 pKa = 3.71KK63 pKa = 11.31GFDD66 pKa = 3.28SGEE69 pKa = 4.01FYY71 pKa = 11.01AVEE74 pKa = 3.81IHH76 pKa = 7.21RR77 pKa = 11.84GVALVNPVTDD87 pKa = 3.66TEE89 pKa = 4.36MVDD92 pKa = 3.79AFVEE96 pKa = 4.18QLALLDD102 pKa = 4.47DD103 pKa = 4.03EE104 pKa = 4.96QIRR107 pKa = 11.84LGNHH111 pKa = 5.3FVYY114 pKa = 10.26DD115 pKa = 3.65QEE117 pKa = 4.4MVTTQEE123 pKa = 5.08FINNDD128 pKa = 3.04AA129 pKa = 4.49

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B6M6|A0A411B6M6_9CAUD Putative DNA polymerase OS=Aeromonas phage ZPAH7B OX=2501745 GN=ZPAH7B_orf00029 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84VITKK6 pKa = 10.23AEE8 pKa = 3.97KK9 pKa = 10.11AVQKK13 pKa = 10.76ARR15 pKa = 11.84RR16 pKa = 11.84LWSAMWKK23 pKa = 10.17RR24 pKa = 11.84LNKK27 pKa = 9.65WGDD30 pKa = 3.35GYY32 pKa = 10.39EE33 pKa = 4.09RR34 pKa = 11.84KK35 pKa = 10.08HH36 pKa = 5.01IARR39 pKa = 11.84RR40 pKa = 11.84AVVVLIASPFVLVEE54 pKa = 3.92MGRR57 pKa = 11.84QAYY60 pKa = 9.25HH61 pKa = 5.57VVADD65 pKa = 4.44TVTEE69 pKa = 4.68LYY71 pKa = 10.43QYY73 pKa = 10.28IRR75 pKa = 11.84HH76 pKa = 5.21NHH78 pKa = 4.63

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

9890

57

1249

341.0

37.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.394 ± 0.677

0.971 ± 0.196

6.299 ± 0.173

6.441 ± 0.288

3.67 ± 0.213

7.846 ± 0.391

2.093 ± 0.223

4.54 ± 0.309

5.905 ± 0.292

8.14 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.306 ± 0.279

3.761 ± 0.201

3.994 ± 0.269

4.661 ± 0.417

5.521 ± 0.182

6.249 ± 0.356

4.944 ± 0.307

6.704 ± 0.341

1.476 ± 0.14

3.084 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski