Erythrobacter sp. EC-HK427

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E7Y2H2|A0A5E7Y2H2_9SPHN NAD(P)H:quinone oxidoreductase OS=Erythrobacter sp. EC-HK427 OX=2038396 GN=ytfG PE=4 SV=1
MM1 pKa = 7.45ARR3 pKa = 11.84PRR5 pKa = 11.84NSLAWLLSDD14 pKa = 4.15SSRR17 pKa = 11.84LVPVAIALAAMAGPVAAQAQTATNTVNVNGPSGLTDD53 pKa = 3.6PVAANNSATDD63 pKa = 3.15SDD65 pKa = 4.66TIIVAPSAGPGTMSYY80 pKa = 8.92ATSGNGLHH88 pKa = 5.36QTSIAILDD96 pKa = 3.7WSTSSIVDD104 pKa = 4.57GIQNGDD110 pKa = 3.59VVNFALTGCRR120 pKa = 11.84TGTLTATFSNVVNAGAMDD138 pKa = 3.6IRR140 pKa = 11.84DD141 pKa = 3.54MEE143 pKa = 4.45TWVGSSAFQAYY154 pKa = 9.18NGPGAGEE161 pKa = 4.67AIHH164 pKa = 6.7TIVDD168 pKa = 3.74DD169 pKa = 5.44LGFTINWSLIVGGQAQPLDD188 pKa = 4.74LIFFDD193 pKa = 5.56AEE195 pKa = 4.08ATNVTNEE202 pKa = 4.55SITATTNGGGWALIEE217 pKa = 4.23NVGGTGYY224 pKa = 9.25TIAGVGSQTISITQTEE240 pKa = 4.95GPSNSPVLLTKK251 pKa = 10.33SASRR255 pKa = 11.84TDD257 pKa = 3.32VSIVAGGNQAVAFGVLLPCDD277 pKa = 4.0YY278 pKa = 11.42SDD280 pKa = 4.85APASYY285 pKa = 10.69GSAAHH290 pKa = 7.5AFRR293 pKa = 11.84EE294 pKa = 4.42DD295 pKa = 3.47AASSGLGLVADD306 pKa = 5.12ASQPILGTGIDD317 pKa = 4.38PEE319 pKa = 5.11FSAQPSVNADD329 pKa = 3.15GDD331 pKa = 4.02DD332 pKa = 3.75TDD334 pKa = 3.96TQGDD338 pKa = 4.08DD339 pKa = 3.61EE340 pKa = 5.46NGVSTFQTLTRR351 pKa = 11.84ATTTYY356 pKa = 9.69TLPAANFTVSGSGNLYY372 pKa = 10.19GWVDD376 pKa = 3.98FDD378 pKa = 5.41GNGTFDD384 pKa = 3.5TDD386 pKa = 3.3EE387 pKa = 4.42FASTTVTGGVPASDD401 pKa = 4.11LVWAGVSGVTSGASTYY417 pKa = 10.55VRR419 pKa = 11.84LRR421 pKa = 11.84FTADD425 pKa = 2.71TLTASGATATAGSGEE440 pKa = 4.2VEE442 pKa = 4.76DD443 pKa = 4.45YY444 pKa = 11.34LVTLTTAVDD453 pKa = 3.8LALTKK458 pKa = 10.67TNTPGVNGEE467 pKa = 3.9VDD469 pKa = 3.49QLDD472 pKa = 3.66DD473 pKa = 3.84TLISGSTTTYY483 pKa = 9.51TIRR486 pKa = 11.84VTNTGPDD493 pKa = 3.7DD494 pKa = 3.75AVGAILTDD502 pKa = 3.53TPGAGLTCDD511 pKa = 3.17AASPVTITGDD521 pKa = 3.64GVPAGSFTIADD532 pKa = 3.73LTGAGITLGTLSTGQTTTLSYY553 pKa = 10.87SCQVNN558 pKa = 3.11

Molecular weight: 56.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E7YPB7|A0A5E7YPB7_9SPHN Membrane protein SmpA OS=Erythrobacter sp. EC-HK427 OX=2038396 GN=ERY430_41445 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.23RR3 pKa = 11.84FKK5 pKa = 10.48VQLMSDD11 pKa = 3.91CTDD14 pKa = 3.45EE15 pKa = 5.14LPARR19 pKa = 11.84CRR21 pKa = 11.84WSIVTASNFNQCVWLMRR38 pKa = 11.84LPLNQTVPVYY48 pKa = 9.57QRR50 pKa = 11.84KK51 pKa = 9.61RR52 pKa = 11.84NEE54 pKa = 4.35LLNQANSVRR63 pKa = 11.84NNRR66 pKa = 11.84PIAIFHH72 pKa = 6.47

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3085

0

3085

970700

20

11255

314.7

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.034 ± 0.084

0.837 ± 0.017

6.117 ± 0.088

6.25 ± 0.044

3.78 ± 0.032

8.942 ± 0.058

1.92 ± 0.026

5.038 ± 0.033

2.674 ± 0.049

9.766 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.028

2.727 ± 0.037

5.158 ± 0.04

3.266 ± 0.024

6.829 ± 0.048

5.306 ± 0.038

5.304 ± 0.045

6.936 ± 0.033

1.438 ± 0.02

2.206 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski