Magnetospirillum marisnigri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A178M7T0|A0A178M7T0_9PROT Xanthine dehydrogenase OS=Magnetospirillum marisnigri OX=1285242 GN=A6A04_07765 PE=4 SV=1
MM1 pKa = 7.35LTLTDD6 pKa = 3.71KK7 pKa = 11.21AVDD10 pKa = 4.17VIRR13 pKa = 11.84DD14 pKa = 3.63VCKK17 pKa = 10.88GEE19 pKa = 3.89YY20 pKa = 8.67QGLRR24 pKa = 11.84IMVQKK29 pKa = 10.48GCSGFSYY36 pKa = 11.43NMGLEE41 pKa = 4.11DD42 pKa = 5.12AAADD46 pKa = 3.87DD47 pKa = 4.31DD48 pKa = 4.4QVLEE52 pKa = 4.24FSDD55 pKa = 3.47VKK57 pKa = 11.3VFVDD61 pKa = 4.0PGSALWLVGATMDD74 pKa = 4.07YY75 pKa = 11.03VDD77 pKa = 4.53NSSMGSGFVFDD88 pKa = 5.23NPNTPPPAASGGGGCSCAVKK108 pKa = 10.63SCGG111 pKa = 4.05

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A178MWW5|A0A178MWW5_9PROT TPR_REGION domain-containing protein OS=Magnetospirillum marisnigri OX=1285242 GN=A6A04_11145 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VIANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QGRR39 pKa = 11.84KK40 pKa = 9.21KK41 pKa = 10.63LSAA44 pKa = 3.91

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4097

0

4097

1336618

29

12448

326.2

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.592 ± 0.059

0.959 ± 0.017

5.865 ± 0.033

5.733 ± 0.053

3.433 ± 0.027

8.63 ± 0.058

2.152 ± 0.021

4.791 ± 0.026

3.446 ± 0.042

10.461 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.026

2.458 ± 0.041

5.127 ± 0.048

3.042 ± 0.028

7.013 ± 0.073

5.431 ± 0.063

5.231 ± 0.088

7.716 ± 0.03

1.313 ± 0.018

1.995 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski