Wuhan cricket virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLR6|A0A1L3KLR6_9VIRU Uncharacterized protein OS=Wuhan cricket virus 2 OX=1923697 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 6.0DD3 pKa = 3.65LPRR6 pKa = 11.84VAPEE10 pKa = 3.64DD11 pKa = 3.94SIFARR16 pKa = 11.84YY17 pKa = 8.63SWEE20 pKa = 4.03FNPDD24 pKa = 3.05SLVPASSRR32 pKa = 11.84QTSRR36 pKa = 11.84RR37 pKa = 11.84LDD39 pKa = 3.77LNPNVQTTPQQPASPPHH56 pKa = 7.03HH57 pKa = 6.76GMRR60 pKa = 11.84TIQEE64 pKa = 3.88WASLVRR70 pKa = 11.84EE71 pKa = 4.84HH72 pKa = 6.07IQKK75 pKa = 10.35RR76 pKa = 11.84MARR79 pKa = 11.84EE80 pKa = 3.68PYY82 pKa = 7.96MTIGEE87 pKa = 4.18QMKK90 pKa = 10.47LLSLEE95 pKa = 4.52DD96 pKa = 3.52FHH98 pKa = 9.38IEE100 pKa = 3.88PSVPAATTLHH110 pKa = 6.68INLPDD115 pKa = 3.57NTEE118 pKa = 3.64EE119 pKa = 4.76AIPVPSDD126 pKa = 3.3LTIDD130 pKa = 3.42TANAKK135 pKa = 9.5LHH137 pKa = 5.39QAEE140 pKa = 4.18QPVIRR145 pKa = 11.84AIGSVAATNVIAEE158 pKa = 4.41TEE160 pKa = 4.09RR161 pKa = 11.84VITNHH166 pKa = 5.26NRR168 pKa = 11.84EE169 pKa = 3.97IMSDD173 pKa = 3.74DD174 pKa = 3.48LHH176 pKa = 7.21KK177 pKa = 10.84VWVMNHH183 pKa = 4.83FVRR186 pKa = 11.84RR187 pKa = 11.84IPLSHH192 pKa = 6.31EE193 pKa = 3.87THH195 pKa = 6.1HH196 pKa = 6.63QRR198 pKa = 11.84GLPLPTFSSSPEE210 pKa = 3.62AKK212 pKa = 9.5IARR215 pKa = 11.84TSTPLLDD222 pKa = 3.88SRR224 pKa = 11.84PPSISPSSWSASDD237 pKa = 3.46IASDD241 pKa = 5.22SPPQLPRR248 pKa = 11.84KK249 pKa = 8.8LAPHH253 pKa = 6.89LPAAPQKK260 pKa = 10.18EE261 pKa = 4.04MRR263 pKa = 11.84YY264 pKa = 9.57LLSATLTSSAPEE276 pKa = 3.45ALFFTEE282 pKa = 4.58EE283 pKa = 3.83ASKK286 pKa = 11.29NPIVGSTLVKK296 pKa = 8.76QTYY299 pKa = 10.5GLLHH303 pKa = 7.2DD304 pKa = 4.81WLAAMSFKK312 pKa = 10.38MRR314 pKa = 11.84TSQVFKK320 pKa = 10.92LRR322 pKa = 11.84NTLACIHH329 pKa = 6.31CHH331 pKa = 5.21QSVNMAYY338 pKa = 10.33NQVTHH343 pKa = 5.68NHH345 pKa = 5.78RR346 pKa = 11.84CVAYY350 pKa = 10.13ALLASFTDD358 pKa = 3.87TVSCQDD364 pKa = 3.86GLHH367 pKa = 6.57HH368 pKa = 7.04LPQYY372 pKa = 10.12SAPLYY377 pKa = 9.59YY378 pKa = 10.33RR379 pKa = 11.84LVRR382 pKa = 11.84NLRR385 pKa = 11.84ISHH388 pKa = 7.13PLRR391 pKa = 11.84QLFSCHH397 pKa = 6.79DD398 pKa = 3.71GVFTPLQTTWLVDD411 pKa = 3.5DD412 pKa = 4.25TT413 pKa = 4.69

Molecular weight: 46.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLY0|A0A1L3KLY0_9VIRU Putative capsid protein OS=Wuhan cricket virus 2 OX=1923697 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 5.04PRR4 pKa = 11.84PYY6 pKa = 10.33LGKK9 pKa = 9.35PANQKK14 pKa = 9.87NPRR17 pKa = 11.84AKK19 pKa = 10.08NLHH22 pKa = 5.37VPRR25 pKa = 11.84APRR28 pKa = 11.84EE29 pKa = 4.02APAAMEE35 pKa = 4.47DD36 pKa = 4.08TPPAPSKK43 pKa = 10.43QASKK47 pKa = 10.95PKK49 pKa = 9.76MPSTLPPPKK58 pKa = 9.72HH59 pKa = 6.48ASNPPRR65 pKa = 11.84KK66 pKa = 9.1EE67 pKa = 3.85DD68 pKa = 3.75FVEE71 pKa = 4.56KK72 pKa = 10.81SPLSDD77 pKa = 5.36AIFQEE82 pKa = 4.29DD83 pKa = 3.85MAVRR87 pKa = 11.84EE88 pKa = 4.37VNLTTRR94 pKa = 11.84QNVSLSAVNEE104 pKa = 4.09LSRR107 pKa = 11.84ATYY110 pKa = 9.94QQMLITDD117 pKa = 4.41PNLAKK122 pKa = 10.11QWTPEE127 pKa = 3.6VHH129 pKa = 7.18DD130 pKa = 4.5YY131 pKa = 11.36YY132 pKa = 10.12VTAMTWLRR140 pKa = 11.84IVALKK145 pKa = 10.63ASSGQDD151 pKa = 3.1LTPAEE156 pKa = 4.26EE157 pKa = 4.45TLLSMSATHH166 pKa = 6.49SFNLTEE172 pKa = 5.19PIRR175 pKa = 11.84LYY177 pKa = 11.09LSGVGVAVTKK187 pKa = 10.66NGQHH191 pKa = 7.28LYY193 pKa = 10.33PSFPPLPTEE202 pKa = 4.24VVDD205 pKa = 6.1GIPGLYY211 pKa = 10.38GPIGEE216 pKa = 4.56ATHH219 pKa = 6.32NLYY222 pKa = 10.9EE223 pKa = 4.73EE224 pKa = 4.55IPALGVSIGMIRR236 pKa = 11.84ASLNPVRR243 pKa = 11.84PIPQWVPPILPVNTNANPNLLGFRR267 pKa = 11.84PIRR270 pKa = 11.84APRR273 pKa = 11.84PEE275 pKa = 4.24AMSITDD281 pKa = 3.43AAEE284 pKa = 3.83INVDD288 pKa = 3.43NFPNYY293 pKa = 8.71PANTGFNMQLLKK305 pKa = 10.82AVSSLIATTSTFKK318 pKa = 10.22VTSTNFSNLAEE329 pKa = 4.47TGSTAQAVLLQPIDD343 pKa = 3.83NDD345 pKa = 3.69DD346 pKa = 3.5PTIRR350 pKa = 11.84TINANLRR357 pKa = 11.84PQSLNHH363 pKa = 7.05DD364 pKa = 3.95PLATFGQAVAFGYY377 pKa = 10.42QLLKK381 pKa = 10.53EE382 pKa = 4.32PCGNNHH388 pKa = 6.43SAWCCITPAAGQVIPQQWIDD408 pKa = 3.38NRR410 pKa = 11.84NDD412 pKa = 3.0RR413 pKa = 11.84RR414 pKa = 11.84NIPAQFQVRR423 pKa = 11.84VFDD426 pKa = 4.43SNSVSAGDD434 pKa = 3.63YY435 pKa = 9.49RR436 pKa = 11.84RR437 pKa = 11.84KK438 pKa = 9.8IVEE441 pKa = 4.04KK442 pKa = 10.3LAKK445 pKa = 10.09KK446 pKa = 10.19SS447 pKa = 3.4

Molecular weight: 49.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2649

338

543

441.5

49.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.72 ± 0.658

1.397 ± 0.19

5.436 ± 0.355

5.059 ± 0.12

3.473 ± 0.228

3.435 ± 0.431

3.662 ± 0.439

4.945 ± 0.359

4.379 ± 0.508

9.475 ± 0.471

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.076 ± 0.156

4.341 ± 0.646

7.739 ± 0.704

4.19 ± 0.435

6.229 ± 0.458

7.324 ± 0.643

7.701 ± 0.707

5.7 ± 0.394

1.246 ± 0.231

3.473 ± 0.642
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski