Gordonia phage EnalisNailo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TY55|A0A4Y5TY55_9CAUD Tail assembly chaperone OS=Gordonia phage EnalisNailo OX=2575354 GN=15 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 9.69IRR5 pKa = 11.84DD6 pKa = 4.01DD7 pKa = 3.89LEE9 pKa = 4.16GVVWVEE15 pKa = 3.63VDD17 pKa = 3.34GKK19 pKa = 10.29GVKK22 pKa = 9.44LAAGDD27 pKa = 4.33TIPSGVDD34 pKa = 3.06VGAHH38 pKa = 6.19LLDD41 pKa = 4.13DD42 pKa = 4.61TADD45 pKa = 3.75EE46 pKa = 4.58DD47 pKa = 5.02QGDD50 pKa = 4.02GDD52 pKa = 3.97DD53 pKa = 4.57TFPEE57 pKa = 4.76GAPEE61 pKa = 4.19LSWKK65 pKa = 10.29GPQLFAYY72 pKa = 9.56AKK74 pKa = 10.25EE75 pKa = 4.01KK76 pKa = 11.14GIDD79 pKa = 3.28LGGATKK85 pKa = 10.59KK86 pKa = 10.82ADD88 pKa = 3.24VLAAIEE94 pKa = 4.16AAQASTGDD102 pKa = 3.55GGQDD106 pKa = 3.45GSGGDD111 pKa = 3.93PDD113 pKa = 4.77AGNHH117 pKa = 4.89GG118 pKa = 3.82

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TYG8|A0A4Y5TYG8_9CAUD Lysin A OS=Gordonia phage EnalisNailo OX=2575354 GN=22 PE=4 SV=1
MM1 pKa = 7.63SVRR4 pKa = 11.84LRR6 pKa = 11.84FNIDD10 pKa = 2.36GFYY13 pKa = 10.89DD14 pKa = 3.72LRR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 3.61PGIVAEE24 pKa = 4.75EE25 pKa = 3.86EE26 pKa = 4.87AIAQQIADD34 pKa = 4.32RR35 pKa = 11.84ANSIGKK41 pKa = 8.2GTYY44 pKa = 10.12AVGSRR49 pKa = 11.84QGRR52 pKa = 11.84KK53 pKa = 9.29APQGRR58 pKa = 11.84WRR60 pKa = 11.84TTVVTADD67 pKa = 3.27ARR69 pKa = 11.84AMANNARR76 pKa = 11.84NNTLIRR82 pKa = 11.84AMEE85 pKa = 3.98

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

16636

39

1874

218.9

23.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.944 ± 0.543

0.962 ± 0.139

6.726 ± 0.241

5.602 ± 0.262

2.693 ± 0.106

8.56 ± 0.456

2.23 ± 0.206

4.466 ± 0.174

3.156 ± 0.235

7.646 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.136

3.138 ± 0.209

5.704 ± 0.307

3.727 ± 0.359

7.856 ± 0.405

5.44 ± 0.29

7.021 ± 0.335

6.792 ± 0.301

2.134 ± 0.154

2.092 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski