Vibrio phage vB_VnaS-AQKL99

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B0A0|A0A7G5B0A0_9CAUD Uncharacterized protein OS=Vibrio phage vB_VnaS-AQKL99 OX=2759205 PE=4 SV=1
MM1 pKa = 7.13LTEE4 pKa = 4.21TMNRR8 pKa = 11.84TIANGLQQLNNEE20 pKa = 4.61TIKK23 pKa = 10.76EE24 pKa = 4.24GNDD27 pKa = 3.15MAGLNFNATNVAPQQAFEE45 pKa = 4.04NLAPGWYY52 pKa = 6.63TVKK55 pKa = 10.14MVEE58 pKa = 4.63SEE60 pKa = 4.41MKK62 pKa = 8.63PTSAGTGSYY71 pKa = 10.4LEE73 pKa = 4.42CTFEE77 pKa = 4.46VLAPQQFAGRR87 pKa = 11.84KK88 pKa = 8.57LWDD91 pKa = 3.73RR92 pKa = 11.84LNLNNPNEE100 pKa = 4.23KK101 pKa = 10.14AVEE104 pKa = 3.74IAYY107 pKa = 7.75QTLSAMCHH115 pKa = 4.98ATGVMQVQDD124 pKa = 3.79SQQLHH129 pKa = 6.89NIPMDD134 pKa = 3.9AKK136 pKa = 10.75VGLSKK141 pKa = 9.61PTEE144 pKa = 4.1QYY146 pKa = 10.24PEE148 pKa = 3.89PRR150 pKa = 11.84NEE152 pKa = 3.73IKK154 pKa = 10.52GYY156 pKa = 9.43RR157 pKa = 11.84ACQGNGGMAPQGQGAAPQQQQWGQPAQQQQQPVQQQQQWGQQQQQAPQQQQQQQQWQQPAEE218 pKa = 4.07QQQQQQQQWQQPAEE232 pKa = 4.11QQQAPQQEE240 pKa = 4.49QQQAAPWGQQTVEE253 pKa = 4.16QQQQQAPQQQQQQVQQQEE271 pKa = 4.89HH272 pKa = 5.68PQQMNMQQPATDD284 pKa = 3.58TQGQQAAQSGVAPWQQGG301 pKa = 2.96

Molecular weight: 33.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B098|A0A7G5B098_9CAUD Uncharacterized protein OS=Vibrio phage vB_VnaS-AQKL99 OX=2759205 PE=4 SV=1
MM1 pKa = 8.29VMAQQKK7 pKa = 9.85RR8 pKa = 11.84EE9 pKa = 3.76EE10 pKa = 4.29WLDD13 pKa = 3.33KK14 pKa = 10.71PKK16 pKa = 10.85RR17 pKa = 11.84CDD19 pKa = 3.03NCQSPRR25 pKa = 11.84IVNADD30 pKa = 2.78NCIIYY35 pKa = 9.37GRR37 pKa = 11.84NYY39 pKa = 10.32GDD41 pKa = 3.48WPRR44 pKa = 11.84IWFCRR49 pKa = 11.84SCNAAVSCHH58 pKa = 6.56PGTNYY63 pKa = 10.37PMGKK67 pKa = 8.3MATLEE72 pKa = 3.91TRR74 pKa = 11.84QARR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84AHH81 pKa = 6.91KK82 pKa = 10.66YY83 pKa = 8.39FDD85 pKa = 3.87KK86 pKa = 10.68IFRR89 pKa = 11.84KK90 pKa = 9.48HH91 pKa = 6.46KK92 pKa = 10.13IMSRR96 pKa = 11.84TDD98 pKa = 2.7AYY100 pKa = 10.64YY101 pKa = 9.75WLASSMMIPKK111 pKa = 9.57SEE113 pKa = 3.91CHH115 pKa = 5.57ISYY118 pKa = 10.56FNAEE122 pKa = 3.59QCEE125 pKa = 4.25RR126 pKa = 11.84VVEE129 pKa = 4.15LSRR132 pKa = 11.84EE133 pKa = 4.29LIRR136 pKa = 11.84RR137 pKa = 11.84AKK139 pKa = 9.61QKK141 pKa = 10.09PRR143 pKa = 11.84TMTHH147 pKa = 3.22VRR149 pKa = 11.84GRR151 pKa = 11.84KK152 pKa = 4.89VTKK155 pKa = 9.19PGHH158 pKa = 5.04RR159 pKa = 11.84RR160 pKa = 11.84KK161 pKa = 10.14LRR163 pKa = 3.56

Molecular weight: 19.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

16588

100

814

267.5

29.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.289 ± 0.338

1.387 ± 0.154

5.986 ± 0.218

6.481 ± 0.305

3.943 ± 0.183

7.469 ± 0.279

2.026 ± 0.183

5.341 ± 0.175

5.992 ± 0.238

8.06 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.159

4.606 ± 0.181

4.395 ± 0.207

4.371 ± 0.481

5.269 ± 0.201

5.528 ± 0.242

6.27 ± 0.228

6.794 ± 0.206

1.61 ± 0.102

3.418 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski