Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; Cupriavidus metallidurans

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6364 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1LLP7|Q1LLP7_CUPMC Oxoglutarate dehydrogenase (succinyl-transferring) OS=Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) OX=266264 GN=sucA PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 6.75PILATLPPSLLALVEE17 pKa = 4.5GSLSNDD23 pKa = 2.79EE24 pKa = 4.32VSSDD28 pKa = 4.01EE29 pKa = 4.14EE30 pKa = 3.84MLAYY34 pKa = 10.24FIDD37 pKa = 3.95NGLTEE42 pKa = 4.41DD43 pKa = 3.76QARR46 pKa = 11.84QALTYY51 pKa = 10.19RR52 pKa = 11.84DD53 pKa = 3.41QYY55 pKa = 11.36LNNIYY60 pKa = 10.76LEE62 pKa = 4.48GFTPITSADD71 pKa = 3.8EE72 pKa = 4.11PLHH75 pKa = 6.08FNPHH79 pKa = 4.38TRR81 pKa = 11.84QFEE84 pKa = 3.94PDD86 pKa = 2.92

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1LPP0|Q1LPP0_CUPMC Heat-inducible transcription repressor HrcA OS=Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) OX=266264 GN=hrcA PE=3 SV=1
MM1 pKa = 7.69LWPLAVRR8 pKa = 11.84KK9 pKa = 9.69KK10 pKa = 10.71KK11 pKa = 10.61LLLPPLTRR19 pKa = 11.84LLRR22 pKa = 11.84LLLLRR27 pKa = 11.84LKK29 pKa = 10.83LRR31 pKa = 11.84LRR33 pKa = 11.84LLLRR37 pKa = 11.84LLTLRR42 pKa = 11.84LRR44 pKa = 11.84LLLRR48 pKa = 11.84LTLRR52 pKa = 11.84LRR54 pKa = 11.84LLTLRR59 pKa = 11.84LRR61 pKa = 11.84LPSSNRR67 pKa = 11.84IRR69 pKa = 11.84PIKK72 pKa = 10.54KK73 pKa = 8.2PTFGSAFFISRR84 pKa = 11.84IFLTPHH90 pKa = 4.82SRR92 pKa = 11.84GRR94 pKa = 3.43

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6364

0

6364

1995538

20

3714

313.6

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.495 ± 0.035

0.968 ± 0.01

5.333 ± 0.023

5.127 ± 0.027

3.495 ± 0.021

8.275 ± 0.037

2.266 ± 0.019

4.638 ± 0.022

3.124 ± 0.027

10.21 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.014

2.765 ± 0.019

5.338 ± 0.026

3.768 ± 0.021

7.084 ± 0.034

5.54 ± 0.023

5.504 ± 0.025

7.643 ± 0.024

1.407 ± 0.014

2.388 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski