Nocardia phage NBR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FHZ2|G9FHZ2_9CAUD Uncharacterized protein OS=Nocardia phage NBR1 OX=1109711 PE=4 SV=1
MM1 pKa = 7.44AWNVTGNIQGPQGPQGDD18 pKa = 4.41EE19 pKa = 4.35GPEE22 pKa = 4.43GPQGIQGPVGPAGGTGPQGEE42 pKa = 4.58VGPAGPQGPAGEE54 pKa = 4.91DD55 pKa = 3.44GAGIEE60 pKa = 4.36IAGSVATYY68 pKa = 11.15AEE70 pKa = 4.71LPSTLGPADD79 pKa = 4.07AGDD82 pKa = 4.67GYY84 pKa = 11.49LVTADD89 pKa = 3.26GKK91 pKa = 11.22LYY93 pKa = 10.07IWDD96 pKa = 3.79GAAFPANGSGVEE108 pKa = 4.15FRR110 pKa = 11.84GPAGPTGSQGPQGVQGPQGVKK131 pKa = 10.5GDD133 pKa = 4.21TGDD136 pKa = 3.4QGPTGSTGSTGATGPRR152 pKa = 11.84GSKK155 pKa = 9.26WFVGAGAPSGVSGSVAGDD173 pKa = 3.46MYY175 pKa = 11.54LDD177 pKa = 3.76TQTGDD182 pKa = 3.18VYY184 pKa = 11.49LLSS187 pKa = 3.93

Molecular weight: 17.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FI03|G9FI03_9CAUD Uncharacterized protein OS=Nocardia phage NBR1 OX=1109711 PE=4 SV=1
MM1 pKa = 7.54PGDD4 pKa = 3.71RR5 pKa = 11.84GHH7 pKa = 6.42GVGRR11 pKa = 11.84GTTNRR16 pKa = 11.84NQRR19 pKa = 11.84GGSASRR25 pKa = 11.84RR26 pKa = 11.84ARR28 pKa = 11.84KK29 pKa = 9.1VWLLATFGNGTVAACAEE46 pKa = 4.52CLTLVDD52 pKa = 4.85FWSISVDD59 pKa = 3.35RR60 pKa = 11.84YY61 pKa = 9.65PVPGCEE67 pKa = 3.92GGRR70 pKa = 11.84YY71 pKa = 6.92VQGNIRR77 pKa = 11.84PMCVPCNSSTGGKK90 pKa = 9.61LGNTRR95 pKa = 11.84RR96 pKa = 11.84DD97 pKa = 3.36LGKK100 pKa = 10.58SKK102 pKa = 11.1SLVSLSNGG110 pKa = 3.16

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14208

39

1180

208.9

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.768 ± 0.377

0.57 ± 0.089

6.905 ± 0.311

5.236 ± 0.258

2.815 ± 0.168

9.312 ± 0.498

1.985 ± 0.216

3.97 ± 0.274

3.301 ± 0.217

8.34 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.121

2.872 ± 0.205

5.976 ± 0.281

3.73 ± 0.17

6.32 ± 0.348

5.018 ± 0.334

6.785 ± 0.293

7.855 ± 0.256

1.964 ± 0.13

2.801 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski