Mucispirillum schaedleri ASF457

Taxonomy: cellular organisms; Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Deferribacteraceae; Mucispirillum; Mucispirillum schaedleri

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2091 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V2QES8|V2QES8_9BACT Peptidyl-tRNA hydrolase OS=Mucispirillum schaedleri ASF457 OX=1379858 GN=pth PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.23KK3 pKa = 10.16YY4 pKa = 10.46VCNVCGWIYY13 pKa = 10.96DD14 pKa = 3.83PAVGIPEE21 pKa = 5.02DD22 pKa = 4.86GIAPGTAFEE31 pKa = 5.32DD32 pKa = 5.04LPDD35 pKa = 4.08DD36 pKa = 4.24WVCPQCFVGKK46 pKa = 10.38DD47 pKa = 3.41EE48 pKa = 5.14FSPLEE53 pKa = 4.13DD54 pKa = 3.16

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V2Q4Q2|V2Q4Q2_9BACT Uncharacterized protein (Fragment) OS=Mucispirillum schaedleri ASF457 OX=1379858 GN=N508_00067 PE=4 SV=1
MM1 pKa = 7.41AQLTLAKK8 pKa = 9.21RR9 pKa = 11.84TNLKK13 pKa = 10.01KK14 pKa = 10.36VRR16 pKa = 11.84KK17 pKa = 8.21QGFRR21 pKa = 11.84ARR23 pKa = 11.84MATKK27 pKa = 10.37GGRR30 pKa = 11.84LVIKK34 pKa = 10.33RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.74GRR41 pKa = 11.84KK42 pKa = 8.66RR43 pKa = 11.84LAVV46 pKa = 3.41

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2091

0

2091

665262

29

1922

318.2

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.952 ± 0.063

1.112 ± 0.02

5.682 ± 0.04

6.554 ± 0.052

4.698 ± 0.045

5.996 ± 0.058

1.595 ± 0.022

9.305 ± 0.056

8.346 ± 0.063

8.667 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.027

6.274 ± 0.063

2.972 ± 0.028

2.68 ± 0.029

3.302 ± 0.031

6.665 ± 0.039

5.086 ± 0.042

6.123 ± 0.043

0.639 ± 0.015

4.642 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski