Flavobacterium sediminis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2952 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8QW20|A0A2U8QW20_9FLAO DNA mismatch repair protein MutL OS=Flavobacterium sediminis OX=2201181 GN=mutL PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 10.51KK3 pKa = 10.51LINNNFRR10 pKa = 11.84FALPLFSLLALASCDD25 pKa = 4.35DD26 pKa = 3.95NMDD29 pKa = 3.7EE30 pKa = 4.66PGYY33 pKa = 9.44PALADD38 pKa = 3.72KK39 pKa = 10.74PVVTLTYY46 pKa = 10.98DD47 pKa = 3.24NLNILEE53 pKa = 5.09LDD55 pKa = 4.07DD56 pKa = 5.16EE57 pKa = 4.82STTGVNEE64 pKa = 4.35NIATFVLTADD74 pKa = 4.85RR75 pKa = 11.84IYY77 pKa = 11.02KK78 pKa = 10.31EE79 pKa = 4.32DD80 pKa = 3.37MKK82 pKa = 11.2FSIKK86 pKa = 10.44LNAAEE91 pKa = 4.28STADD95 pKa = 3.53AKK97 pKa = 11.28DD98 pKa = 3.62FTISLSEE105 pKa = 4.04ADD107 pKa = 4.69LDD109 pKa = 4.23YY110 pKa = 9.94GTDD113 pKa = 3.47GAFVATIPANTLSTTFTVAANFDD136 pKa = 3.95IYY138 pKa = 10.79PEE140 pKa = 4.15STEE143 pKa = 4.19NLVFDD148 pKa = 4.99IKK150 pKa = 11.07PMADD154 pKa = 3.4LNGTIAGSSKK164 pKa = 10.39KK165 pKa = 10.51VEE167 pKa = 3.97LTIGNSTSDD176 pKa = 3.97EE177 pKa = 4.15LVLILDD183 pKa = 3.83WNGDD187 pKa = 3.43STYY190 pKa = 11.49TNITDD195 pKa = 3.94PNDD198 pKa = 3.91PYY200 pKa = 11.41EE201 pKa = 4.44STLADD206 pKa = 3.45YY207 pKa = 11.32DD208 pKa = 4.13FDD210 pKa = 5.43LEE212 pKa = 4.65VYY214 pKa = 10.54NSGFSLVDD222 pKa = 3.5YY223 pKa = 10.07SWSDD227 pKa = 3.13SPEE230 pKa = 3.79QVNFATSNADD240 pKa = 2.88GLYY243 pKa = 9.95FVIAGFYY250 pKa = 8.07STYY253 pKa = 10.57GAVPMLPINFDD264 pKa = 3.45VTLTIAKK271 pKa = 9.43PGVFVYY277 pKa = 10.27DD278 pKa = 3.81YY279 pKa = 11.44DD280 pKa = 4.31MSGIWNSTDD289 pKa = 3.33GGDD292 pKa = 4.79DD293 pKa = 3.49EE294 pKa = 6.28GNVDD298 pKa = 4.06AYY300 pKa = 10.03QVPVYY305 pKa = 9.82FVKK308 pKa = 8.6TTTNGVATYY317 pKa = 9.73EE318 pKa = 4.29VYY320 pKa = 11.04NLADD324 pKa = 3.91DD325 pKa = 4.06TLLASGKK332 pKa = 10.38SSTDD336 pKa = 2.94LKK338 pKa = 11.37VYY340 pKa = 10.15FNSFKK345 pKa = 10.5RR346 pKa = 11.84VKK348 pKa = 10.42

Molecular weight: 38.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8QVF3|A0A2U8QVF3_9FLAO DUF350 domain-containing protein OS=Flavobacterium sediminis OX=2201181 GN=DI487_08335 PE=3 SV=1
MM1 pKa = 7.46GGKK4 pKa = 9.29ALCFYY9 pKa = 10.64LFYY12 pKa = 11.18ARR14 pKa = 11.84WVWEE18 pKa = 3.98NKK20 pKa = 8.99RR21 pKa = 11.84VKK23 pKa = 9.58TMCLTRR29 pKa = 11.84GKK31 pKa = 10.33QGLSVGWKK39 pKa = 8.39TPLQGEE45 pKa = 4.37ARR47 pKa = 11.84TGRR50 pKa = 11.84AQRR53 pKa = 11.84TQRR56 pKa = 11.84SVPILGFGLDD66 pKa = 3.84FAA68 pKa = 6.63

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2952

0

2952

964564

28

3877

326.7

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.216 ± 0.042

0.812 ± 0.017

5.425 ± 0.039

6.837 ± 0.055

5.383 ± 0.045

6.19 ± 0.057

1.722 ± 0.022

7.834 ± 0.044

7.845 ± 0.073

9.149 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.136 ± 0.021

6.246 ± 0.05

3.312 ± 0.023

3.634 ± 0.026

3.148 ± 0.031

6.396 ± 0.047

6.023 ± 0.07

6.378 ± 0.036

1.006 ± 0.017

4.308 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski