Pirellulimonas nuda

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Pirellulimonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518DDH3|A0A518DDH3_9BACT Inositol 2-dehydrogenase OS=Pirellulimonas nuda OX=2528009 GN=iolG_7 PE=4 SV=1
MM1 pKa = 7.73IFRR4 pKa = 11.84PYY6 pKa = 11.19ALTLALVALPGLSSAQSVLNIDD28 pKa = 4.18FGGGPNASGAGNFVGLAAYY47 pKa = 9.45GADD50 pKa = 3.68PAGAAALWNGINDD63 pKa = 4.32PLGDD67 pKa = 4.26AGTTNPLFDD76 pKa = 4.5SLNTPTSVSYY86 pKa = 10.89RR87 pKa = 11.84HH88 pKa = 5.03TTAGGGGTFIKK99 pKa = 10.22IPPAQEE105 pKa = 4.0VGTGDD110 pKa = 3.49PPQFLNLMNDD120 pKa = 3.99YY121 pKa = 10.68IWFNGGGTGRR131 pKa = 11.84TATISGLTPNATYY144 pKa = 11.06DD145 pKa = 3.78LYY147 pKa = 11.25AYY149 pKa = 9.76GQGDD153 pKa = 4.37TFTSLTSNQASKK165 pKa = 9.28FTVNGIEE172 pKa = 4.03KK173 pKa = 7.3TTTYY177 pKa = 9.82DD178 pKa = 3.01TDD180 pKa = 3.72AGGMLANDD188 pKa = 4.01GLLAEE193 pKa = 4.69NIEE196 pKa = 4.44YY197 pKa = 10.74VRR199 pKa = 11.84FTAVNSGPSGQIQISHH215 pKa = 5.97VNPTGGGFSAFNGLQIVGTFLAPPVPLNLEE245 pKa = 4.18VNTTNGTVTIQNNNQIPVQFDD266 pKa = 3.79VYY268 pKa = 10.99VIEE271 pKa = 4.45TSAGTLSKK279 pKa = 10.94SGWNSLSDD287 pKa = 3.56QNFDD291 pKa = 3.46ATAGAADD298 pKa = 4.25GDD300 pKa = 4.42YY301 pKa = 11.18NDD303 pKa = 5.58DD304 pKa = 3.99GAVDD308 pKa = 3.48AADD311 pKa = 3.51YY312 pKa = 8.25TVWRR316 pKa = 11.84DD317 pKa = 3.63SLNLPSAGNLEE328 pKa = 4.12NDD330 pKa = 4.71PIGGTVGAAQYY341 pKa = 9.61DD342 pKa = 3.48QWKK345 pKa = 10.52NGFGDD350 pKa = 3.69AGGTATGWDD359 pKa = 3.63EE360 pKa = 5.46AFGTSNSLLAEE371 pKa = 4.96FYY373 pKa = 10.64LGSGDD378 pKa = 4.32KK379 pKa = 9.89PASSIAAGQSLNLGPAFTSASADD402 pKa = 3.22GTLTFNYY409 pKa = 10.19YY410 pKa = 8.71DD411 pKa = 3.85TQASANMVGTVSFVNAAIGGNTVPEE436 pKa = 4.7PGCVWLLLAGAVSLGWRR453 pKa = 11.84LRR455 pKa = 11.84TPTNGRR461 pKa = 11.84CGG463 pKa = 3.35

Molecular weight: 47.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518DCP5|A0A518DCP5_9BACT PEP-CTERM domain-containing protein OS=Pirellulimonas nuda OX=2528009 GN=Pla175_26390 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84FAAASPEE9 pKa = 4.13PEE11 pKa = 4.05SLICRR16 pKa = 11.84SVQVEE21 pKa = 4.4DD22 pKa = 4.57GVATLLVEE30 pKa = 4.61SGRR33 pKa = 11.84QTAHH37 pKa = 6.7CPSCGEE43 pKa = 3.75PSRR46 pKa = 11.84RR47 pKa = 11.84VHH49 pKa = 6.04SRR51 pKa = 11.84YY52 pKa = 9.6VRR54 pKa = 11.84NLKK57 pKa = 10.17DD58 pKa = 3.9LSWHH62 pKa = 5.84GLQVRR67 pKa = 11.84LVWRR71 pKa = 11.84SRR73 pKa = 11.84KK74 pKa = 9.43LFCRR78 pKa = 11.84NAGCQQRR85 pKa = 11.84VFTEE89 pKa = 4.35RR90 pKa = 11.84LPKK93 pKa = 9.89VAQPHH98 pKa = 5.72ARR100 pKa = 11.84QTTRR104 pKa = 11.84SEE106 pKa = 3.7EE107 pKa = 4.01ALRR110 pKa = 11.84AIAMACGGLPGARR123 pKa = 11.84PANRR127 pKa = 11.84LGMATSGDD135 pKa = 3.58SLLRR139 pKa = 11.84LLRR142 pKa = 11.84RR143 pKa = 11.84AGHH146 pKa = 6.52KK147 pKa = 10.32KK148 pKa = 8.78PACPQVLGVDD158 pKa = 4.68DD159 pKa = 3.44IAFRR163 pKa = 11.84KK164 pKa = 9.12RR165 pKa = 11.84ATYY168 pKa = 8.23GTILCDD174 pKa = 3.43LVRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84PIHH182 pKa = 6.26LLPEE186 pKa = 4.18RR187 pKa = 11.84SKK189 pKa = 11.3EE190 pKa = 4.12SLSAWLSKK198 pKa = 10.76HH199 pKa = 6.04SGVKK203 pKa = 9.98IISRR207 pKa = 11.84DD208 pKa = 2.98RR209 pKa = 11.84GEE211 pKa = 4.26YY212 pKa = 8.7YY213 pKa = 10.33RR214 pKa = 11.84QGASEE219 pKa = 4.29GAPHH223 pKa = 7.46ALQVADD229 pKa = 4.75RR230 pKa = 11.84WHH232 pKa = 6.59LLCNLRR238 pKa = 11.84EE239 pKa = 4.1ALVRR243 pKa = 11.84WLGRR247 pKa = 11.84CSRR250 pKa = 11.84EE251 pKa = 3.73WPMDD255 pKa = 3.31

Molecular weight: 28.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5103

0

5103

1869859

29

3095

366.4

39.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.064 ± 0.052

1.117 ± 0.014

6.064 ± 0.029

5.814 ± 0.029

3.376 ± 0.018

8.85 ± 0.038

2.016 ± 0.015

3.905 ± 0.024

2.805 ± 0.03

9.991 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.014

2.714 ± 0.028

5.839 ± 0.028

3.718 ± 0.022

7.237 ± 0.038

5.798 ± 0.029

5.217 ± 0.029

7.527 ± 0.027

1.574 ± 0.014

2.404 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski