Lawsonia intracellularis (strain PHE/MN1-00)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Lawsonia; Lawsonia intracellularis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1MSG0|Q1MSG0_LAWIP NA OS=Lawsonia intracellularis (strain PHE/MN1-00) OX=363253 GN=LI0009 PE=4 SV=1
MM1 pKa = 7.39VSTDD5 pKa = 3.48VIDD8 pKa = 4.72ALGHH12 pKa = 6.34LLNIPGLSFNEE23 pKa = 4.43DD24 pKa = 4.08LLCLLSVNNDD34 pKa = 3.15NVDD37 pKa = 3.43VTIEE41 pKa = 4.23TNEE44 pKa = 4.21SGDD47 pKa = 3.77LLYY50 pKa = 11.29VCAHH54 pKa = 7.38LGTMPDD60 pKa = 4.13DD61 pKa = 4.99DD62 pKa = 4.16NAAAAIALLFAQANAVLTAMNKK84 pKa = 9.46GVLVLDD90 pKa = 4.22YY91 pKa = 11.02NGQGLMLVKK100 pKa = 10.21LYY102 pKa = 10.71EE103 pKa = 4.12MKK105 pKa = 10.23HH106 pKa = 5.36WEE108 pKa = 4.86LEE110 pKa = 4.16DD111 pKa = 3.76CLEE114 pKa = 5.66SILQLFNDD122 pKa = 3.27AGEE125 pKa = 4.36WKK127 pKa = 10.31NRR129 pKa = 11.84LYY131 pKa = 11.15LPDD134 pKa = 4.72FGLGGMDD141 pKa = 4.45FDD143 pKa = 6.59LDD145 pKa = 4.02TSSSGSDD152 pKa = 2.84QFMRR156 pKa = 11.84VV157 pKa = 2.94

Molecular weight: 17.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q1MPR3|RL3_LAWIP 50S ribosomal protein L3 OS=Lawsonia intracellularis (strain PHE/MN1-00) OX=363253 GN=rplC PE=3 SV=1
MM1 pKa = 8.13AIRR4 pKa = 11.84NLKK7 pKa = 7.94PTSPGCRR14 pKa = 11.84FQTISDD20 pKa = 3.93FKK22 pKa = 10.93EE23 pKa = 4.17ITCSSPEE30 pKa = 3.63KK31 pKa = 10.49SLTYY35 pKa = 10.23GLCKK39 pKa = 10.07KK40 pKa = 10.48AGRR43 pKa = 11.84NNHH46 pKa = 4.71GHH48 pKa = 4.93ITSRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84GGGAKK59 pKa = 9.66RR60 pKa = 11.84LYY62 pKa = 10.55RR63 pKa = 11.84LIDD66 pKa = 3.81FKK68 pKa = 10.88RR69 pKa = 11.84DD70 pKa = 3.45KK71 pKa = 11.11VGVPAKK77 pKa = 10.02VAQIEE82 pKa = 4.15YY83 pKa = 10.53DD84 pKa = 3.53PNRR87 pKa = 11.84TARR90 pKa = 11.84IALLHH95 pKa = 5.76YY96 pKa = 10.51VDD98 pKa = 3.9GEE100 pKa = 3.97KK101 pKa = 10.21RR102 pKa = 11.84YY103 pKa = 9.94ILAPVGIKK111 pKa = 10.07QGDD114 pKa = 3.81IIEE117 pKa = 4.61TGVDD121 pKa = 3.27ADD123 pKa = 3.83IKK125 pKa = 10.4PGNSLPMLHH134 pKa = 7.02IPIGTLIHH142 pKa = 6.67NIEE145 pKa = 4.46LNPGRR150 pKa = 11.84GGQLCRR156 pKa = 11.84SAGAYY161 pKa = 8.29AQLVARR167 pKa = 11.84EE168 pKa = 4.21GKK170 pKa = 9.91YY171 pKa = 10.76ALLRR175 pKa = 11.84MPSGEE180 pKa = 3.95VRR182 pKa = 11.84KK183 pKa = 10.22VLASGAATVGQVGNIHH199 pKa = 6.77HH200 pKa = 6.65EE201 pKa = 4.39NISLGKK207 pKa = 10.13AGRR210 pKa = 11.84NRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PKK219 pKa = 10.49VRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.73HH230 pKa = 7.25PLGGGEE236 pKa = 4.15GRR238 pKa = 11.84SSGGRR243 pKa = 11.84HH244 pKa = 5.52PVSPWGMPTKK254 pKa = 10.4GYY256 pKa = 7.8KK257 pKa = 9.22TRR259 pKa = 11.84DD260 pKa = 3.2KK261 pKa = 10.64KK262 pKa = 11.12KK263 pKa = 8.6PSSRR267 pKa = 11.84LIVKK271 pKa = 10.17RR272 pKa = 11.84RR273 pKa = 11.84GQKK276 pKa = 9.78

Molecular weight: 30.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1341

1

1342

474690

67

8746

353.7

39.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.336 ± 0.073

1.349 ± 0.024

4.436 ± 0.055

6.247 ± 0.165

4.328 ± 0.068

6.463 ± 0.064

2.306 ± 0.033

8.599 ± 0.126

6.332 ± 0.123

10.602 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.036

4.622 ± 0.06

4.349 ± 0.046

4.193 ± 0.082

4.087 ± 0.049

7.243 ± 0.099

5.959 ± 0.05

5.837 ± 0.056

1.147 ± 0.028

3.353 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski