Peribacillus cavernae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Peribacillus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0VQ23|A0A3S0VQ23_9BACI MBL fold metallo-hydrolase OS=Peribacillus cavernae OX=1674310 GN=ELQ35_07975 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.03FTSLIYY9 pKa = 10.65SLLIMVLLIGCASNNSSPLEE29 pKa = 3.99NEE31 pKa = 4.31EE32 pKa = 5.52LNQQTDD38 pKa = 3.68TEE40 pKa = 4.38EE41 pKa = 4.37KK42 pKa = 10.86ANEE45 pKa = 3.85TALDD49 pKa = 3.79YY50 pKa = 11.56DD51 pKa = 4.19GDD53 pKa = 4.6GGFLWKK59 pKa = 10.29VVNGEE64 pKa = 3.72TTAYY68 pKa = 10.62VLGSVHH74 pKa = 7.08LGHH77 pKa = 7.05EE78 pKa = 4.42DD79 pKa = 4.04YY80 pKa = 11.27YY81 pKa = 10.75PLVTEE86 pKa = 4.18IEE88 pKa = 4.17EE89 pKa = 4.58AYY91 pKa = 10.1EE92 pKa = 4.18SSDD95 pKa = 4.26VILPEE100 pKa = 3.8INMFEE105 pKa = 3.83VDD107 pKa = 3.14ITKK110 pKa = 10.88EE111 pKa = 4.18EE112 pKa = 4.15INEE115 pKa = 3.91MALFDD120 pKa = 6.05DD121 pKa = 4.67GTTLDD126 pKa = 3.8EE127 pKa = 4.41VLSEE131 pKa = 3.89EE132 pKa = 4.81SYY134 pKa = 11.86AKK136 pKa = 10.69LSDD139 pKa = 3.02IFEE142 pKa = 4.27THH144 pKa = 6.08RR145 pKa = 11.84MNVEE149 pKa = 4.04DD150 pKa = 5.0FKK152 pKa = 11.29QYY154 pKa = 10.73QPWFVEE160 pKa = 4.05SLLTEE165 pKa = 4.82FVAEE169 pKa = 4.13EE170 pKa = 4.29SDD172 pKa = 3.44LSSEE176 pKa = 4.37YY177 pKa = 11.11GVDD180 pKa = 3.99LYY182 pKa = 11.12FLQRR186 pKa = 11.84ALEE189 pKa = 4.21DD190 pKa = 3.58DD191 pKa = 3.81KK192 pKa = 11.7EE193 pKa = 4.71IIEE196 pKa = 4.7LEE198 pKa = 4.37TIEE201 pKa = 4.63GQYY204 pKa = 11.55EE205 pKa = 4.29MLTGFSMDD213 pKa = 3.6TQVEE217 pKa = 4.21TLEE220 pKa = 4.85DD221 pKa = 3.88YY222 pKa = 10.87IGAYY226 pKa = 9.29EE227 pKa = 5.43DD228 pKa = 3.42QADD231 pKa = 3.38WLNRR235 pKa = 11.84LGYY238 pKa = 10.19NWVHH242 pKa = 6.02GNSNRR247 pKa = 11.84DD248 pKa = 3.31SLVNQLSDD256 pKa = 3.17SFEE259 pKa = 4.47GVDD262 pKa = 4.23EE263 pKa = 5.04EE264 pKa = 4.69YY265 pKa = 10.79QQEE268 pKa = 4.29LNDD271 pKa = 3.59NRR273 pKa = 11.84NINMANKK280 pKa = 9.78LDD282 pKa = 5.21EE283 pKa = 4.8ILQSDD288 pKa = 3.54NGQTYY293 pKa = 8.94FAIIGSLHH301 pKa = 5.17TVIDD305 pKa = 4.04PSVLSEE311 pKa = 4.37LEE313 pKa = 3.92EE314 pKa = 4.44KK315 pKa = 10.83GYY317 pKa = 10.69DD318 pKa = 3.44VEE320 pKa = 5.65RR321 pKa = 11.84IYY323 pKa = 11.59

Molecular weight: 37.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433H891|A0A433H891_9BACI Uncharacterized protein OS=Peribacillus cavernae OX=1674310 GN=ELQ35_21600 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4169

0

4169

1212771

17

1737

290.9

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.238 ± 0.04

0.739 ± 0.01

5.077 ± 0.03

7.506 ± 0.042

4.41 ± 0.03

7.223 ± 0.033

2.089 ± 0.018

7.843 ± 0.039

6.978 ± 0.036

9.634 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.019

4.361 ± 0.025

3.775 ± 0.021

3.581 ± 0.025

4.166 ± 0.025

6.118 ± 0.026

5.293 ± 0.026

6.883 ± 0.031

0.99 ± 0.015

3.364 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski