Lachnospiraceae bacterium OM04-12BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417UA16|A0A417UA16_9FIRM Cupin domain-containing protein OS=Lachnospiraceae bacterium OM04-12BH OX=2292272 GN=DXB46_05585 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.49KK3 pKa = 9.78KK4 pKa = 7.97WTTGKK9 pKa = 10.35IIGVSMLLVLGVVILWIAFVASVYY33 pKa = 10.63QLATVIEE40 pKa = 4.98GIEE43 pKa = 4.28RR44 pKa = 11.84ITAGRR49 pKa = 11.84AGITGDD55 pKa = 3.66SDD57 pKa = 5.49DD58 pKa = 3.82MDD60 pKa = 5.71GYY62 pKa = 11.12DD63 pKa = 5.81IEE65 pKa = 6.0DD66 pKa = 4.07FLNGDD71 pKa = 3.7SDD73 pKa = 3.77EE74 pKa = 4.52EE75 pKa = 4.88YY76 pKa = 11.39YY77 pKa = 11.1DD78 pKa = 3.83FEE80 pKa = 4.71QDD82 pKa = 3.3IRR84 pKa = 11.84DD85 pKa = 3.9DD86 pKa = 3.51LSYY89 pKa = 10.85EE90 pKa = 4.12VEE92 pKa = 4.05LEE94 pKa = 4.09EE95 pKa = 5.47FDD97 pKa = 5.64KK98 pKa = 11.29EE99 pKa = 5.75DD100 pKa = 3.68YY101 pKa = 10.55DD102 pKa = 4.53CPEE105 pKa = 3.88GTEE108 pKa = 3.75ARR110 pKa = 11.84LAITYY115 pKa = 8.21PVVTGDD121 pKa = 3.31IPNVEE126 pKa = 4.25EE127 pKa = 4.4INRR130 pKa = 11.84KK131 pKa = 9.64LYY133 pKa = 11.02EE134 pKa = 4.2EE135 pKa = 4.11VTAADD140 pKa = 4.15EE141 pKa = 4.68YY142 pKa = 9.64ITAVADD148 pKa = 3.77LMEE151 pKa = 6.21DD152 pKa = 3.61GDD154 pKa = 4.14SFEE157 pKa = 4.63YY158 pKa = 10.6QSEE161 pKa = 4.87CYY163 pKa = 7.67VTYY166 pKa = 9.76MDD168 pKa = 5.0EE169 pKa = 5.79DD170 pKa = 3.73ILSVAFQEE178 pKa = 4.8RR179 pKa = 11.84GILNDD184 pKa = 3.45SMIGAVIVSMNFDD197 pKa = 4.04LQTGLPVGNTGILDD211 pKa = 3.64IDD213 pKa = 4.24DD214 pKa = 4.1EE215 pKa = 4.45FAIDD219 pKa = 3.55FRR221 pKa = 11.84KK222 pKa = 10.21RR223 pKa = 11.84CEE225 pKa = 4.03EE226 pKa = 3.72QNGEE230 pKa = 4.23INGLNYY236 pKa = 10.68YY237 pKa = 10.31SDD239 pKa = 3.61QQIRR243 pKa = 11.84EE244 pKa = 4.09YY245 pKa = 9.95LTNEE249 pKa = 3.15NSLIIFYY256 pKa = 9.48TPLGMEE262 pKa = 3.78VGINYY267 pKa = 9.81DD268 pKa = 4.09DD269 pKa = 3.81GWATVTYY276 pKa = 10.56KK277 pKa = 10.73DD278 pKa = 3.63YY279 pKa = 11.77EE280 pKa = 4.31KK281 pKa = 10.32FTKK284 pKa = 10.27QFF286 pKa = 3.23

Molecular weight: 32.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417U6I5|A0A417U6I5_9FIRM ABC transporter permease OS=Lachnospiraceae bacterium OM04-12BH OX=2292272 GN=DXB46_07875 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.78TRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.97VHH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3107

0

3107

999537

16

2994

321.7

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.592 ± 0.046

1.508 ± 0.017

5.516 ± 0.034

7.943 ± 0.047

4.147 ± 0.033

7.075 ± 0.04

1.686 ± 0.019

7.3 ± 0.039

6.589 ± 0.041

9.228 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.157 ± 0.023

4.1 ± 0.033

3.244 ± 0.022

3.306 ± 0.025

4.651 ± 0.042

5.77 ± 0.035

5.346 ± 0.033

6.725 ± 0.039

0.882 ± 0.014

4.234 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski