Mycobacterium phage Pharaoh

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W310|A0A481W310_9CAUD Uncharacterized protein OS=Mycobacterium phage Pharaoh OX=2530140 GN=1 PE=4 SV=1
MM1 pKa = 7.33TMIEE5 pKa = 4.25TLGDD9 pKa = 3.35IAQMARR15 pKa = 11.84NVKK18 pKa = 9.86HH19 pKa = 5.82GRR21 pKa = 11.84EE22 pKa = 4.16PGDD25 pKa = 3.18NTYY28 pKa = 11.23NCTWAAAALHH38 pKa = 6.99AYY40 pKa = 8.43TEE42 pKa = 4.58VVGGSDD48 pKa = 3.88NDD50 pKa = 3.6VATQLSDD57 pKa = 3.72LLGDD61 pKa = 4.5LMHH64 pKa = 7.1LADD67 pKa = 4.3AAGVDD72 pKa = 4.16FDD74 pKa = 4.15EE75 pKa = 5.26AVNKK79 pKa = 11.0AEE81 pKa = 4.03FNYY84 pKa = 10.33DD85 pKa = 3.21AEE87 pKa = 4.85VNGEE91 pKa = 3.94

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W2S8|A0A481W2S8_9CAUD Uncharacterized protein OS=Mycobacterium phage Pharaoh OX=2530140 GN=43 PE=4 SV=1
MM1 pKa = 7.55VFRR4 pKa = 11.84LWPSFLPGVVTLSVSPVTWPFVVALRR30 pKa = 11.84STALYY35 pKa = 10.62LSTRR39 pKa = 11.84HH40 pKa = 5.71NRR42 pKa = 11.84IKK44 pKa = 10.81APWPAATDD52 pKa = 3.15QRR54 pKa = 11.84AIHH57 pKa = 5.98QIGAGAVKK65 pKa = 10.23SLSRR69 pKa = 11.84QKK71 pKa = 10.51VASGVAAAGTVAVGGLAFALSFTALSDD98 pKa = 3.94LASHH102 pKa = 7.07AGVTPGQAWMVPLVIDD118 pKa = 4.05GGVIVATAATVALQGRR134 pKa = 11.84YY135 pKa = 8.14AWFLLILGSLVSVAGNVAHH154 pKa = 6.7AQPHH158 pKa = 6.04GLLPMLIAAVPPLWLLAATHH178 pKa = 5.73LTVMLSRR185 pKa = 11.84PKK187 pKa = 10.44DD188 pKa = 3.22AALVEE193 pKa = 4.25EE194 pKa = 4.96SEE196 pKa = 4.3IAPLRR201 pKa = 11.84AAA203 pKa = 4.46

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15710

47

1005

196.4

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.267 ± 0.491

0.751 ± 0.123

6.085 ± 0.196

6.244 ± 0.346

3.393 ± 0.212

8.32 ± 0.565

1.935 ± 0.185

4.946 ± 0.171

4.437 ± 0.201

7.982 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.119

3.221 ± 0.142

5.767 ± 0.282

3.851 ± 0.254

6.206 ± 0.331

5.595 ± 0.181

6.569 ± 0.222

7.078 ± 0.217

1.897 ± 0.155

2.89 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski