Azoarcus sp. CC-YHH838

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4AWT3|A0A4S4AWT3_9RHOO AbrB family transcriptional regulator OS=Azoarcus sp. CC-YHH838 OX=2565930 GN=E6C76_10610 PE=4 SV=1
TT1 pKa = 6.9ATATDD6 pKa = 3.79NASNTATDD14 pKa = 3.8TDD16 pKa = 3.88TAGYY20 pKa = 10.53DD21 pKa = 3.5LLPTVGVSFEE31 pKa = 4.03ISDD34 pKa = 3.81AGVGTLTGTSEE45 pKa = 4.18HH46 pKa = 6.03ATSVSVTITGSDD58 pKa = 3.57GSNLGPFPATLNPDD72 pKa = 3.62GSWSVDD78 pKa = 3.32LSGEE82 pKa = 4.21SFDD85 pKa = 5.45EE86 pKa = 4.29EE87 pKa = 4.25VTYY90 pKa = 9.34TATATATDD98 pKa = 3.88NASNTATGSDD108 pKa = 3.21TAGYY112 pKa = 10.53DD113 pKa = 3.51LLPTVGVSFEE123 pKa = 4.03ISDD126 pKa = 3.81AGVGTLTGTSEE137 pKa = 4.18HH138 pKa = 6.03ATSVSVTITGSDD150 pKa = 3.57GSNLGPFPATLNPDD164 pKa = 3.62GSWSVDD170 pKa = 3.32LSGEE174 pKa = 4.21SFDD177 pKa = 5.45EE178 pKa = 4.29EE179 pKa = 4.25VTYY182 pKa = 9.34TATATATDD190 pKa = 3.88NASNTATDD198 pKa = 3.8TDD200 pKa = 3.88TAGYY204 pKa = 10.53DD205 pKa = 3.5LLPTVGVSFEE215 pKa = 4.03ISDD218 pKa = 3.81AGVGTLTGTSEE229 pKa = 4.18HH230 pKa = 6.03ATSVSVTITGSDD242 pKa = 3.57GSNLGPFPATLNPDD256 pKa = 3.62GTWSVDD262 pKa = 3.44LSGEE266 pKa = 4.21SFDD269 pKa = 5.45EE270 pKa = 4.29EE271 pKa = 4.25VTYY274 pKa = 9.34TATATATDD282 pKa = 3.88NASNTATGSDD292 pKa = 3.31TASYY296 pKa = 10.68DD297 pKa = 3.58LNAPPVAIDD306 pKa = 4.49DD307 pKa = 4.24PTGQPYY313 pKa = 10.25SVSLGSLGSGWSNPDD328 pKa = 3.47SNHH331 pKa = 6.52SLTDD335 pKa = 3.78LSAWKK340 pKa = 10.49ADD342 pKa = 3.42GTAGEE347 pKa = 5.3LYY349 pKa = 10.43QSGDD353 pKa = 3.22MRR355 pKa = 11.84GVKK358 pKa = 10.35GSPRR362 pKa = 11.84GGALDD367 pKa = 3.81QEE369 pKa = 4.65PDD371 pKa = 3.28QLQSNNSGQSEE382 pKa = 4.38AIEE385 pKa = 4.26LKK387 pKa = 10.81FSGNLDD393 pKa = 3.17QATFSVSHH401 pKa = 6.94LYY403 pKa = 11.03SNEE406 pKa = 3.68NGYY409 pKa = 10.9GEE411 pKa = 4.02IGRR414 pKa = 11.84WVAYY418 pKa = 9.99FDD420 pKa = 3.94GVEE423 pKa = 4.13VASGEE428 pKa = 4.2FRR430 pKa = 11.84FNDD433 pKa = 3.81LTNSTHH439 pKa = 6.64SGTFAIDD446 pKa = 3.04TGGRR450 pKa = 11.84LFNSVRR456 pKa = 11.84FEE458 pKa = 3.95AVHH461 pKa = 6.41NGDD464 pKa = 3.61SSGDD468 pKa = 3.42SSGYY472 pKa = 10.29FLTGFAGTGPEE483 pKa = 4.32VANGTYY489 pKa = 9.98LVSVGDD495 pKa = 3.76TLNVSEE501 pKa = 5.69LEE503 pKa = 4.73GILANDD509 pKa = 4.04SDD511 pKa = 4.73PEE513 pKa = 4.1NDD515 pKa = 3.74PLSIVAVNGSALTVGEE531 pKa = 4.79WITLPSGAKK540 pKa = 10.32LMLNADD546 pKa = 3.78GSFSYY551 pKa = 9.19DD552 pKa = 3.0TNNQFGHH559 pKa = 6.84LAAGEE564 pKa = 4.05WATDD568 pKa = 3.26SFQYY572 pKa = 10.29TISDD576 pKa = 3.5GMGGEE581 pKa = 4.41DD582 pKa = 3.44TATVTVTVVGTGVPVDD598 pKa = 3.55TSVTDD603 pKa = 3.83GNEE606 pKa = 4.28VISTAEE612 pKa = 3.99DD613 pKa = 3.26AVLTGNVLANTDD625 pKa = 3.99TNVGTPAVSSFSVAGDD641 pKa = 3.34PAIYY645 pKa = 10.19DD646 pKa = 3.88AGDD649 pKa = 3.21TATISGVGTLTIGADD664 pKa = 3.22GEE666 pKa = 4.73YY667 pKa = 10.15RR668 pKa = 11.84FEE670 pKa = 4.2PAQDD674 pKa = 3.48YY675 pKa = 10.66SGPVPTVTYY684 pKa = 9.31TVTNGVQSDD693 pKa = 3.85TSTLEE698 pKa = 3.72ITVTPVADD706 pKa = 3.68QPEE709 pKa = 4.43VSVDD713 pKa = 3.46VQVAGSVVQTLTTANVLGTPTGFTVTAYY741 pKa = 10.02KK742 pKa = 10.67DD743 pKa = 3.68GEE745 pKa = 4.19PATLSVRR752 pKa = 11.84SSGSPTGFGVAGSASDD768 pKa = 3.78GASEE772 pKa = 4.32EE773 pKa = 4.15IGNGEE778 pKa = 4.24VLRR781 pKa = 11.84VQLQTAAEE789 pKa = 4.29SVSFRR794 pKa = 11.84LAWLASGEE802 pKa = 4.15YY803 pKa = 10.19AKK805 pKa = 10.98YY806 pKa = 10.63VLIYY810 pKa = 10.42TDD812 pKa = 3.8GTSEE816 pKa = 4.56SVILNGNSAVGGYY829 pKa = 9.65DD830 pKa = 3.04QVGSVITRR838 pKa = 11.84NAPAGKK844 pKa = 9.72LIAAIEE850 pKa = 4.24FTTPGVGEE858 pKa = 3.71AHH860 pKa = 6.02YY861 pKa = 10.85AGNNDD866 pKa = 3.33YY867 pKa = 11.1LVYY870 pKa = 10.15QVSYY874 pKa = 10.45VAASSYY880 pKa = 10.33EE881 pKa = 4.06LNIVATPTDD890 pKa = 3.62NYY892 pKa = 8.53HH893 pKa = 6.08TEE895 pKa = 4.35SITEE899 pKa = 4.05LVVVVPVGITLSAGTHH915 pKa = 6.44DD916 pKa = 5.84GSGKK920 pKa = 7.4WTLPLDD926 pKa = 3.74GAGAYY931 pKa = 7.23TVQVDD936 pKa = 3.96PVTKK940 pKa = 9.17EE941 pKa = 3.79VSVSGLSMNVPSDD954 pKa = 3.5FSGEE958 pKa = 3.94LTLTVTAKK966 pKa = 10.74AADD969 pKa = 3.89GTDD972 pKa = 3.32TAEE975 pKa = 4.8GSTSLNIEE983 pKa = 4.18RR984 pKa = 11.84GTAGNDD990 pKa = 3.43TLVGTDD996 pKa = 3.84GADD999 pKa = 3.15ILIGGAGDD1007 pKa = 4.01DD1008 pKa = 4.08VLTGGLGADD1017 pKa = 3.36VFVWRR1022 pKa = 11.84LGDD1025 pKa = 3.34QGTNSQPATDD1035 pKa = 4.02RR1036 pKa = 11.84VTDD1039 pKa = 4.01FNLGQGDD1046 pKa = 4.1VLDD1049 pKa = 5.66LRR1051 pKa = 11.84DD1052 pKa = 4.44LLDD1055 pKa = 3.71GHH1057 pKa = 6.68SGSLDD1062 pKa = 3.07HH1063 pKa = 6.37YY1064 pKa = 10.68LKK1066 pKa = 10.85VEE1068 pKa = 4.04EE1069 pKa = 4.52SSGNTVLTISTTGNVNASHH1088 pKa = 6.84DD1089 pKa = 3.88QVIVLDD1095 pKa = 4.4GVTGLIDD1102 pKa = 4.38GGLTSQSAIIQDD1114 pKa = 5.1LINKK1118 pKa = 8.08QAIQIDD1124 pKa = 3.74

Molecular weight: 114.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4AYU7|A0A4S4AYU7_9RHOO Integrating conjugative element protein OS=Azoarcus sp. CC-YHH838 OX=2565930 GN=E6C76_12590 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4235

0

4235

1419603

35

3709

335.2

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.182 ± 0.057

0.929 ± 0.013

5.419 ± 0.026

5.918 ± 0.035

3.468 ± 0.023

8.587 ± 0.051

2.312 ± 0.021

4.323 ± 0.029

2.579 ± 0.026

11.242 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.019

2.509 ± 0.027

5.153 ± 0.034

3.51 ± 0.023

7.92 ± 0.046

4.958 ± 0.035

4.855 ± 0.042

7.344 ± 0.031

1.411 ± 0.018

2.28 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski