Schizophyllum commune (strain H4-8 / FGSC 9210) (Split gill fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Schizophyllaceae; Schizophyllum; Schizophyllum commune

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8Q8W0|D8Q8W0_SCHCM Alpha-amylase OS=Schizophyllum commune (strain H4-8 / FGSC 9210) OX=578458 GN=SCHCODRAFT_77509 PE=3 SV=1
MM1 pKa = 7.7PVPPRR6 pKa = 11.84RR7 pKa = 11.84PNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LPAVDD17 pKa = 3.9SDD19 pKa = 4.17EE20 pKa = 4.51EE21 pKa = 4.78DD22 pKa = 3.89GEE24 pKa = 4.21EE25 pKa = 4.43DD26 pKa = 3.21GGGVAEE32 pKa = 4.73VIEE35 pKa = 4.38IQDD38 pKa = 4.54DD39 pKa = 3.98EE40 pKa = 6.56DD41 pKa = 6.14DD42 pKa = 4.25GASDD46 pKa = 3.14EE47 pKa = 5.03GYY49 pKa = 9.42PVPHH53 pKa = 7.06HH54 pKa = 6.69APHH57 pKa = 6.52VDD59 pKa = 4.38FPYY62 pKa = 10.97GLNSGPRR69 pKa = 11.84VHH71 pKa = 7.41DD72 pKa = 4.5PYY74 pKa = 11.69AHH76 pKa = 6.88LDD78 pKa = 3.57EE79 pKa = 6.08RR80 pKa = 11.84LDD82 pKa = 3.65IQDD85 pKa = 3.87DD86 pKa = 3.9RR87 pKa = 11.84SVVEE91 pKa = 6.03DD92 pKa = 4.09GDD94 pKa = 3.96QVDD97 pKa = 5.81DD98 pKa = 4.44GDD100 pKa = 4.37QVDD103 pKa = 4.11DD104 pKa = 4.42DD105 pKa = 5.28EE106 pKa = 6.04YY107 pKa = 11.71DD108 pKa = 3.22HH109 pKa = 6.64RR110 pKa = 11.84HH111 pKa = 6.45PYY113 pKa = 9.34AEE115 pKa = 5.2HH116 pKa = 6.47YY117 pKa = 10.36PPDD120 pKa = 3.91HH121 pKa = 7.31LDD123 pKa = 3.32RR124 pKa = 11.84GGNNYY129 pKa = 9.74YY130 pKa = 10.74GQGAFDD136 pKa = 5.96DD137 pKa = 5.18DD138 pKa = 4.38DD139 pKa = 4.64EE140 pKa = 5.14EE141 pKa = 5.33YY142 pKa = 10.28PDD144 pKa = 3.59YY145 pKa = 11.53GGVEE149 pKa = 4.34DD150 pKa = 4.7EE151 pKa = 5.2DD152 pKa = 4.11EE153 pKa = 4.35EE154 pKa = 5.59AGIVYY159 pKa = 9.73PSDD162 pKa = 3.15EE163 pKa = 3.95EE164 pKa = 4.55RR165 pKa = 11.84YY166 pKa = 10.15SDD168 pKa = 4.21EE169 pKa = 4.41EE170 pKa = 4.33YY171 pKa = 11.38

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8Q5C2|D8Q5C2_SCHCM Uncharacterized protein (Fragment) OS=Schizophyllum commune (strain H4-8 / FGSC 9210) OX=578458 GN=SCHCODRAFT_109170 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84KK3 pKa = 8.65IWFPSLPTVMPPRR16 pKa = 11.84SRR18 pKa = 11.84PRR20 pKa = 11.84RR21 pKa = 11.84SLTLRR26 pKa = 11.84PKK28 pKa = 10.58SPSSTKK34 pKa = 7.82QRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84MSRR42 pKa = 11.84KK43 pKa = 9.02RR44 pKa = 11.84LMRR47 pKa = 11.84SPPPRR52 pKa = 11.84KK53 pKa = 8.98STTSPLNRR61 pKa = 11.84SLLMRR66 pKa = 11.84RR67 pKa = 11.84TFMSQRR73 pKa = 11.84LMPRR77 pKa = 11.84RR78 pKa = 11.84SLPRR82 pKa = 11.84MLQLKK87 pKa = 9.88RR88 pKa = 11.84RR89 pKa = 11.84TRR91 pKa = 11.84QRR93 pKa = 11.84RR94 pKa = 11.84IMPRR98 pKa = 11.84RR99 pKa = 11.84QKK101 pKa = 10.32RR102 pKa = 11.84VKK104 pKa = 9.96KK105 pKa = 10.3LRR107 pKa = 11.84RR108 pKa = 11.84QSTTWPPSRR117 pKa = 11.84LNRR120 pKa = 11.84PRR122 pKa = 11.84AQLSLLSRR130 pKa = 11.84RR131 pKa = 11.84LRR133 pKa = 11.84PRR135 pKa = 11.84KK136 pKa = 7.77PTSPLRR142 pKa = 11.84LLRR145 pKa = 11.84NPARR149 pKa = 11.84LLPRR153 pKa = 11.84SPLLPRR159 pKa = 11.84SLSSPRR165 pKa = 11.84TPPSPPPSATISPTWSACWRR185 pKa = 3.75

Molecular weight: 22.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13124

4

13128

5882520

23

5159

448.1

49.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.873 ± 0.021

1.323 ± 0.008

5.899 ± 0.015

5.993 ± 0.021

3.463 ± 0.013

6.527 ± 0.021

2.548 ± 0.01

4.322 ± 0.014

4.182 ± 0.019

9.138 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.008

2.99 ± 0.011

6.793 ± 0.03

3.582 ± 0.016

6.882 ± 0.022

8.013 ± 0.029

5.869 ± 0.013

6.32 ± 0.016

1.442 ± 0.008

2.731 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski