Paenibacillus larvae subsp. larvae DSM 25430

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus larvae; Paenibacillus larvae subsp. larvae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9WBE8|V9WBE8_9BACL Elongation factor P OS=Paenibacillus larvae subsp. larvae DSM 25430 OX=697284 GN=efp PE=3 SV=1
MM1 pKa = 7.55TEE3 pKa = 3.92FGTVVLEE10 pKa = 4.17GKK12 pKa = 8.54EE13 pKa = 4.19FKK15 pKa = 10.22LTGDD19 pKa = 3.75ADD21 pKa = 3.75FTNCVLGGWYY31 pKa = 9.28TDD33 pKa = 4.14FNDD36 pKa = 3.41ASEE39 pKa = 4.52GEE41 pKa = 4.38EE42 pKa = 4.1YY43 pKa = 10.25QFEE46 pKa = 4.29MSAPGLDD53 pKa = 3.45NEE55 pKa = 4.6GNEE58 pKa = 4.09VTVYY62 pKa = 10.31WIFTDD67 pKa = 3.52IKK69 pKa = 10.28GEE71 pKa = 4.0KK72 pKa = 9.89GKK74 pKa = 10.9EE75 pKa = 3.88SLDD78 pKa = 3.42EE79 pKa = 3.95YY80 pKa = 11.24DD81 pKa = 3.71YY82 pKa = 11.78DD83 pKa = 3.82NVDD86 pKa = 3.14RR87 pKa = 11.84VVYY90 pKa = 9.84VV91 pKa = 3.45

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9WE19|V9WE19_9BACL Uncharacterized protein OS=Paenibacillus larvae subsp. larvae DSM 25430 OX=697284 GN=ERIC2_c36351 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84PTFNPNNRR10 pKa = 11.84KK11 pKa = 9.34RR12 pKa = 11.84KK13 pKa = 8.34KK14 pKa = 9.1NHH16 pKa = 4.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.03NGRR28 pKa = 11.84KK29 pKa = 8.83VLSARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3693

0

3693

1008502

26

5369

273.1

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.312 ± 0.038

0.919 ± 0.014

4.915 ± 0.033

7.188 ± 0.051

4.157 ± 0.035

7.23 ± 0.038

2.25 ± 0.024

7.116 ± 0.044

6.421 ± 0.041

9.995 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.02

3.793 ± 0.024

3.965 ± 0.025

3.958 ± 0.034

5.047 ± 0.036

6.032 ± 0.029

5.232 ± 0.025

6.996 ± 0.028

1.135 ± 0.016

3.403 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski