[Clostridium] cellulosi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillospiraceae incertae sedis

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078KR11|A0A078KR11_9FIRM Chorismate synthase OS=[Clostridium] cellulosi OX=29343 GN=aroC PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.6AFIDD6 pKa = 3.6RR7 pKa = 11.84DD8 pKa = 3.65GCIACGLCVEE18 pKa = 4.92TCPDD22 pKa = 3.32VFRR25 pKa = 11.84FADD28 pKa = 3.61DD29 pKa = 3.78GFAEE33 pKa = 4.73VYY35 pKa = 10.79VDD37 pKa = 5.51DD38 pKa = 4.75VPAGQEE44 pKa = 3.95DD45 pKa = 4.42KK46 pKa = 11.22AVEE49 pKa = 4.0AQEE52 pKa = 4.09NCPTSVITVKK62 pKa = 10.89EE63 pKa = 4.06EE64 pKa = 3.61

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078KQC6|A0A078KQC6_9FIRM Putative membrane protein OS=[Clostridium] cellulosi OX=29343 GN=CCDG5_0176 PE=4 SV=1
MM1 pKa = 6.87QAAAHH6 pKa = 5.95LRR8 pKa = 11.84YY9 pKa = 10.07ARR11 pKa = 11.84ISPRR15 pKa = 11.84KK16 pKa = 7.69VQVVLDD22 pKa = 4.55LIRR25 pKa = 11.84NKK27 pKa = 9.58PANVAAAILQHH38 pKa = 4.97TRR40 pKa = 11.84KK41 pKa = 9.76AASEE45 pKa = 4.02PLLKK49 pKa = 10.58LLKK52 pKa = 10.41SAMANAEE59 pKa = 4.32NNHH62 pKa = 5.57GMDD65 pKa = 3.14VANCYY70 pKa = 9.13VSKK73 pKa = 10.87CFVCPGPTLKK83 pKa = 10.52RR84 pKa = 11.84IRR86 pKa = 11.84PRR88 pKa = 11.84AQGRR92 pKa = 11.84AFQILKK98 pKa = 8.53RR99 pKa = 11.84TSHH102 pKa = 5.41ITLVLEE108 pKa = 4.07EE109 pKa = 4.92RR110 pKa = 11.84EE111 pKa = 4.14NSS113 pKa = 3.42

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2007

0

2007

639157

31

3289

318.5

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.891 ± 0.063

1.498 ± 0.022

5.535 ± 0.045

6.488 ± 0.061

4.339 ± 0.045

7.14 ± 0.049

1.58 ± 0.022

8.138 ± 0.057

7.306 ± 0.045

8.988 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.023

4.779 ± 0.041

3.665 ± 0.028

2.665 ± 0.026

4.431 ± 0.049

6.444 ± 0.052

5.212 ± 0.034

6.778 ± 0.038

0.785 ± 0.019

3.734 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski