Amino acid dipepetide frequency for Nonlabens spongiae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.776AlaAla: 4.776 ± 0.092	0.55AlaCys: 0.55 ± 0.025	3.838AlaAsp: 3.838 ± 0.072	4.071AlaGlu: 4.071 ± 0.07	3.346AlaPhe: 3.346 ± 0.06	4.904AlaGly: 4.904 ± 0.103	1.162AlaHis: 1.162 ± 0.034	5.06AlaIle: 5.06 ± 0.084	3.981AlaLys: 3.981 ± 0.08	6.32AlaLeu: 6.32 ± 0.083	1.678AlaMet: 1.678 ± 0.047	2.832AlaAsn: 2.832 ± 0.065	2.062AlaPro: 2.062 ± 0.052	3.039AlaGln: 3.039 ± 0.064	2.681AlaArg: 2.681 ± 0.059	4.276AlaSer: 4.276 ± 0.071	3.9AlaThr: 3.9 ± 0.08	4.552AlaVal: 4.552 ± 0.074	0.593AlaTrp: 0.593 ± 0.029	2.436AlaTyr: 2.436 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.493CysAla: 0.493 ± 0.028	0.118CysCys: 0.118 ± 0.012	0.491CysAsp: 0.491 ± 0.024	0.503CysGlu: 0.503 ± 0.024	0.371CysPhe: 0.371 ± 0.016	0.624CysGly: 0.624 ± 0.029	0.157CysHis: 0.157 ± 0.012	0.55CysIle: 0.55 ± 0.024	0.442CysLys: 0.442 ± 0.023	0.65CysLeu: 0.65 ± 0.026	0.158CysMet: 0.158 ± 0.012	0.344CysAsn: 0.344 ± 0.019	0.318CysPro: 0.318 ± 0.024	0.221CysGln: 0.221 ± 0.015	0.251CysArg: 0.251 ± 0.016	0.553CysSer: 0.553 ± 0.03	0.387CysThr: 0.387 ± 0.02	0.436CysVal: 0.436 ± 0.022	0.078CysTrp: 0.078 ± 0.009	0.294CysTyr: 0.294 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.116AspAla: 4.116 ± 0.091	0.466AspCys: 0.466 ± 0.023	3.679AspAsp: 3.679 ± 0.088	4.169AspGlu: 4.169 ± 0.074	3.713AspPhe: 3.713 ± 0.071	4.355AspGly: 4.355 ± 0.162	1.279AspHis: 1.279 ± 0.039	4.265AspIle: 4.265 ± 0.078	3.642AspLys: 3.642 ± 0.078	6.263AspLeu: 6.263 ± 0.08	1.312AspMet: 1.312 ± 0.04	3.015AspAsn: 3.015 ± 0.087	2.286AspPro: 2.286 ± 0.066	2.525AspGln: 2.525 ± 0.058	2.955AspArg: 2.955 ± 0.06	3.802AspSer: 3.802 ± 0.077	2.923AspThr: 2.923 ± 0.071	3.853AspVal: 3.853 ± 0.075	0.743AspTrp: 0.743 ± 0.031	2.957AspTyr: 2.957 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	4.111GluAla: 4.111 ± 0.083	0.355GluCys: 0.355 ± 0.018	3.984GluAsp: 3.984 ± 0.078	5.19GluGlu: 5.19 ± 0.101	3.134GluPhe: 3.134 ± 0.063	3.572GluGly: 3.572 ± 0.063	1.3GluHis: 1.3 ± 0.041	5.497GluIle: 5.497 ± 0.094	5.3GluLys: 5.3 ± 0.097	6.716GluLeu: 6.716 ± 0.095	1.781GluMet: 1.781 ± 0.044	4.584GluAsn: 4.584 ± 0.087	1.827GluPro: 1.827 ± 0.046	2.772GluGln: 2.772 ± 0.053	3.048GluArg: 3.048 ± 0.062	3.788GluSer: 3.788 ± 0.06	3.458GluThr: 3.458 ± 0.067	4.7GluVal: 4.7 ± 0.073	0.693GluTrp: 0.693 ± 0.027	2.397GluTyr: 2.397 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.299PheAla: 3.299 ± 0.062	0.432PheCys: 0.432 ± 0.022	3.348PheAsp: 3.348 ± 0.068	3.485PheGlu: 3.485 ± 0.06	2.434PhePhe: 2.434 ± 0.065	2.996PheGly: 2.996 ± 0.062	0.846PheHis: 0.846 ± 0.03	3.603PheIle: 3.603 ± 0.067	3.534PheLys: 3.534 ± 0.067	4.485PheLeu: 4.485 ± 0.084	1.087PheMet: 1.087 ± 0.035	3.064PheAsn: 3.064 ± 0.06	1.623PhePro: 1.623 ± 0.04	1.681PheGln: 1.681 ± 0.041	1.772PheArg: 1.772 ± 0.045	3.467PheSer: 3.467 ± 0.061	3.004PheThr: 3.004 ± 0.052	2.841PheVal: 2.841 ± 0.05	0.557PheTrp: 0.557 ± 0.025	2.211PheTyr: 2.211 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.309GlyAla: 4.309 ± 0.088	0.533GlyCys: 0.533 ± 0.028	3.856GlyAsp: 3.856 ± 0.09	3.591GlyGlu: 3.591 ± 0.069	3.482GlyPhe: 3.482 ± 0.068	4.607GlyGly: 4.607 ± 0.155	1.137GlyHis: 1.137 ± 0.042	5.022GlyIle: 5.022 ± 0.087	4.509GlyLys: 4.509 ± 0.082	5.796GlyLeu: 5.796 ± 0.094	1.758GlyMet: 1.758 ± 0.044	3.655GlyAsn: 3.655 ± 0.079	1.337GlyPro: 1.337 ± 0.04	2.097GlyGln: 2.097 ± 0.046	2.545GlyArg: 2.545 ± 0.06	4.448GlySer: 4.448 ± 0.096	4.125GlyThr: 4.125 ± 0.112	4.512GlyVal: 4.512 ± 0.076	0.74GlyTrp: 0.74 ± 0.033	2.795GlyTyr: 2.795 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.06HisAla: 1.06 ± 0.033	0.165HisCys: 0.165 ± 0.013	1.134HisAsp: 1.134 ± 0.038	1.179HisGlu: 1.179 ± 0.043	1.143HisPhe: 1.143 ± 0.039	1.133HisGly: 1.133 ± 0.039	0.585HisHis: 0.585 ± 0.025	1.303HisIle: 1.303 ± 0.042	1.216HisLys: 1.216 ± 0.04	1.884HisLeu: 1.884 ± 0.051	0.367HisMet: 0.367 ± 0.021	0.869HisAsn: 0.869 ± 0.028	0.875HisPro: 0.875 ± 0.032	0.704HisGln: 0.704 ± 0.029	0.816HisArg: 0.816 ± 0.028	1.143HisSer: 1.143 ± 0.035	0.84HisThr: 0.84 ± 0.032	1.06HisVal: 1.06 ± 0.032	0.189HisTrp: 0.189 ± 0.015	0.806HisTyr: 0.806 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.633IleAla: 5.633 ± 0.086	0.638IleCys: 0.638 ± 0.03	5.16IleAsp: 5.16 ± 0.074	5.148IleGlu: 5.148 ± 0.078	3.38IlePhe: 3.38 ± 0.066	4.442IleGly: 4.442 ± 0.08	1.343IleHis: 1.343 ± 0.039	5.199IleIle: 5.199 ± 0.092	5.046IleLys: 5.046 ± 0.099	6.614IleLeu: 6.614 ± 0.102	1.43IleMet: 1.43 ± 0.041	4.213IleAsn: 4.213 ± 0.076	2.97IlePro: 2.97 ± 0.056	2.402IleGln: 2.402 ± 0.05	2.507IleArg: 2.507 ± 0.054	5.051IleSer: 5.051 ± 0.087	4.377IleThr: 4.377 ± 0.08	4.505IleVal: 4.505 ± 0.075	0.637IleTrp: 0.637 ± 0.031	2.791IleTyr: 2.791 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	4.544LysAla: 4.544 ± 0.09	0.349LysCys: 0.349 ± 0.021	3.972LysAsp: 3.972 ± 0.08	5.307LysGlu: 5.307 ± 0.11	2.711LysPhe: 2.711 ± 0.062	3.847LysGly: 3.847 ± 0.072	1.303LysHis: 1.303 ± 0.041	5.133LysIle: 5.133 ± 0.087	5.88LysLys: 5.88 ± 0.108	6.097LysLeu: 6.097 ± 0.089	1.891LysMet: 1.891 ± 0.051	4.392LysAsn: 4.392 ± 0.075	2.186LysPro: 2.186 ± 0.055	2.425LysGln: 2.425 ± 0.062	3.083LysArg: 3.083 ± 0.06	4.406LysSer: 4.406 ± 0.078	3.819LysThr: 3.819 ± 0.069	4.167LysVal: 4.167 ± 0.078	0.7LysTrp: 0.7 ± 0.028	2.608LysTyr: 2.608 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	5.821LeuAla: 5.821 ± 0.08	0.68LeuCys: 0.68 ± 0.03	5.906LeuAsp: 5.906 ± 0.106	6.573LeuGlu: 6.573 ± 0.1	4.572LeuPhe: 4.572 ± 0.096	6.001LeuGly: 6.001 ± 0.087	1.553LeuHis: 1.553 ± 0.045	6.902LeuIle: 6.902 ± 0.106	7.209LeuLys: 7.209 ± 0.117	8.468LeuLeu: 8.468 ± 0.137	2.166LeuMet: 2.166 ± 0.054	5.416LeuAsn: 5.416 ± 0.09	3.501LeuPro: 3.501 ± 0.06	3.193LeuGln: 3.193 ± 0.059	3.971LeuArg: 3.971 ± 0.076	6.756LeuSer: 6.756 ± 0.087	4.987LeuThr: 4.987 ± 0.069	5.448LeuVal: 5.448 ± 0.076	0.927LeuTrp: 0.927 ± 0.037	3.14LeuTyr: 3.14 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	1.646MetAla: 1.646 ± 0.049	0.127MetCys: 0.127 ± 0.011	1.44MetAsp: 1.44 ± 0.042	1.563MetGlu: 1.563 ± 0.043	0.766MetPhe: 0.766 ± 0.028	1.523MetGly: 1.523 ± 0.043	0.415MetHis: 0.415 ± 0.021	1.72MetIle: 1.72 ± 0.047	2.085MetLys: 2.085 ± 0.048	1.97MetLeu: 1.97 ± 0.05	0.709MetMet: 0.709 ± 0.034	1.354MetAsn: 1.354 ± 0.036	0.928MetPro: 0.928 ± 0.031	0.851MetGln: 0.851 ± 0.029	1.066MetArg: 1.066 ± 0.033	1.548MetSer: 1.548 ± 0.04	1.263MetThr: 1.263 ± 0.037	1.523MetVal: 1.523 ± 0.041	0.174MetTrp: 0.174 ± 0.015	0.628MetTyr: 0.628 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.718AsnAla: 3.718 ± 0.074	0.464AsnCys: 0.464 ± 0.023	3.402AsnAsp: 3.402 ± 0.079	3.537AsnGlu: 3.537 ± 0.069	2.681AsnPhe: 2.681 ± 0.051	3.993AsnGly: 3.993 ± 0.104	1.034AsnHis: 1.034 ± 0.028	3.766AsnIle: 3.766 ± 0.064	3.353AsnLys: 3.353 ± 0.07	5.254AsnLeu: 5.254 ± 0.086	1.157AsnMet: 1.157 ± 0.034	3.149AsnAsn: 3.149 ± 0.08	2.645AsnPro: 2.645 ± 0.063	2.236AsnGln: 2.236 ± 0.051	2.468AsnArg: 2.468 ± 0.06	3.527AsnSer: 3.527 ± 0.075	2.965AsnThr: 2.965 ± 0.074	3.352AsnVal: 3.352 ± 0.075	0.707AsnTrp: 0.707 ± 0.028	2.48AsnTyr: 2.48 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	2.287ProAla: 2.287 ± 0.059	0.239ProCys: 0.239 ± 0.024	2.491ProAsp: 2.491 ± 0.064	2.988ProGlu: 2.988 ± 0.064	1.817ProPhe: 1.817 ± 0.039	1.965ProGly: 1.965 ± 0.042	0.659ProHis: 0.659 ± 0.025	2.481ProIle: 2.481 ± 0.051	2.053ProLys: 2.053 ± 0.052	2.957ProLeu: 2.957 ± 0.051	0.697ProMet: 0.697 ± 0.027	1.899ProAsn: 1.899 ± 0.058	0.874ProPro: 0.874 ± 0.038	1.34ProGln: 1.34 ± 0.031	1.096ProArg: 1.096 ± 0.034	2.254ProSer: 2.254 ± 0.056	1.886ProThr: 1.886 ± 0.046	2.548ProVal: 2.548 ± 0.049	0.375ProTrp: 0.375 ± 0.021	1.377ProTyr: 1.377 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.371GlnAla: 2.371 ± 0.051	0.168GlnCys: 0.168 ± 0.012	2.169GlnAsp: 2.169 ± 0.051	2.749GlnGlu: 2.749 ± 0.059	1.8GlnPhe: 1.8 ± 0.047	2.073GlnGly: 2.073 ± 0.052	0.623GlnHis: 0.623 ± 0.022	2.837GlnIle: 2.837 ± 0.055	2.671GlnLys: 2.671 ± 0.072	3.617GlnLeu: 3.617 ± 0.068	0.935GlnMet: 0.935 ± 0.03	2.232GlnAsn: 2.232 ± 0.052	1.337GlnPro: 1.337 ± 0.035	1.665GlnGln: 1.665 ± 0.045	1.61GlnArg: 1.61 ± 0.043	2.195GlnSer: 2.195 ± 0.054	1.95GlnThr: 1.95 ± 0.053	2.473GlnVal: 2.473 ± 0.052	0.378GlnTrp: 0.378 ± 0.019	1.298GlnTyr: 1.298 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.5ArgAla: 2.5 ± 0.052	0.224ArgCys: 0.224 ± 0.014	2.556ArgAsp: 2.556 ± 0.057	3.11ArgGlu: 3.11 ± 0.062	2.329ArgPhe: 2.329 ± 0.051	2.297ArgGly: 2.297 ± 0.048	0.757ArgHis: 0.757 ± 0.03	3.174ArgIle: 3.174 ± 0.051	3.267ArgLys: 3.267 ± 0.068	3.869ArgLeu: 3.869 ± 0.068	1.056ArgMet: 1.056 ± 0.036	2.395ArgAsn: 2.395 ± 0.045	1.265ArgPro: 1.265 ± 0.038	1.354ArgGln: 1.354 ± 0.037	1.845ArgArg: 1.845 ± 0.049	2.586ArgSer: 2.586 ± 0.063	2.088ArgThr: 2.088 ± 0.05	2.581ArgVal: 2.581 ± 0.055	0.429ArgTrp: 0.429 ± 0.024	1.895ArgTyr: 1.895 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	3.987SerAla: 3.987 ± 0.07	0.623SerCys: 0.623 ± 0.028	3.72SerAsp: 3.72 ± 0.069	3.999SerGlu: 3.999 ± 0.059	3.466SerPhe: 3.466 ± 0.065	4.953SerGly: 4.953 ± 0.099	1.146SerHis: 1.146 ± 0.035	4.975SerIle: 4.975 ± 0.069	4.45SerLys: 4.45 ± 0.074	6.145SerLeu: 6.145 ± 0.084	1.477SerMet: 1.477 ± 0.039	3.618SerAsn: 3.618 ± 0.081	1.984SerPro: 1.984 ± 0.048	2.492SerGln: 2.492 ± 0.05	2.927SerArg: 2.927 ± 0.062	4.676SerSer: 4.676 ± 0.11	3.722SerThr: 3.722 ± 0.071	3.871SerVal: 3.871 ± 0.068	0.742SerTrp: 0.742 ± 0.028	2.794SerTyr: 2.794 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.008ThrAla: 4.008 ± 0.085	0.392ThrCys: 0.392 ± 0.02	3.688ThrAsp: 3.688 ± 0.088	3.455ThrGlu: 3.455 ± 0.058	2.675ThrPhe: 2.675 ± 0.062	4.26ThrGly: 4.26 ± 0.095	0.975ThrHis: 0.975 ± 0.038	4.23ThrIle: 4.23 ± 0.074	2.819ThrLys: 2.819 ± 0.064	5.169ThrLeu: 5.169 ± 0.088	1.087ThrMet: 1.087 ± 0.034	2.662ThrAsn: 2.662 ± 0.07	2.319ThrPro: 2.319 ± 0.057	1.95ThrGln: 1.95 ± 0.05	1.961ThrArg: 1.961 ± 0.042	3.758ThrSer: 3.758 ± 0.076	3.434ThrThr: 3.434 ± 0.087	3.986ThrVal: 3.986 ± 0.099	0.507ThrTrp: 0.507 ± 0.028	2.317ThrTyr: 2.317 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	4.541ValAla: 4.541 ± 0.075	0.508ValCys: 0.508 ± 0.022	4.215ValAsp: 4.215 ± 0.083	4.372ValGlu: 4.372 ± 0.076	3.212ValPhe: 3.212 ± 0.059	3.962ValGly: 3.962 ± 0.063	1.087ValHis: 1.087 ± 0.037	4.634ValIle: 4.634 ± 0.074	4.057ValLys: 4.057 ± 0.076	5.91ValLeu: 5.91 ± 0.085	1.526ValMet: 1.526 ± 0.042	3.319ValAsn: 3.319 ± 0.066	2.329ValPro: 2.329 ± 0.041	2.184ValGln: 2.184 ± 0.051	2.484ValArg: 2.484 ± 0.05	4.27ValSer: 4.27 ± 0.075	3.781ValThr: 3.781 ± 0.09	4.442ValVal: 4.442 ± 0.075	0.565ValTrp: 0.565 ± 0.025	2.35ValTyr: 2.35 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.514TrpAla: 0.514 ± 0.024	0.086TrpCys: 0.086 ± 0.01	0.625TrpAsp: 0.625 ± 0.025	0.663TrpGlu: 0.663 ± 0.026	0.538TrpPhe: 0.538 ± 0.026	0.59TrpGly: 0.59 ± 0.029	0.223TrpHis: 0.223 ± 0.016	0.781TrpIle: 0.781 ± 0.032	0.734TrpLys: 0.734 ± 0.034	1.049TrpLeu: 1.049 ± 0.031	0.338TrpMet: 0.338 ± 0.017	0.67TrpAsn: 0.67 ± 0.028	0.222TrpPro: 0.222 ± 0.017	0.416TrpGln: 0.416 ± 0.019	0.484TrpArg: 0.484 ± 0.021	0.685TrpSer: 0.685 ± 0.035	0.594TrpThr: 0.594 ± 0.026	0.591TrpVal: 0.591 ± 0.025	0.153TrpTrp: 0.153 ± 0.015	0.416TrpTyr: 0.416 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.323TyrAla: 2.323 ± 0.056	0.319TyrCys: 0.319 ± 0.019	2.645TyrAsp: 2.645 ± 0.052	2.559TyrGlu: 2.559 ± 0.048	2.2TyrPhe: 2.2 ± 0.05	2.756TyrGly: 2.756 ± 0.066	0.887TyrHis: 0.887 ± 0.03	2.353TyrIle: 2.353 ± 0.054	2.573TyrLys: 2.573 ± 0.054	3.959TyrLeu: 3.959 ± 0.066	0.693TyrMet: 0.693 ± 0.029	2.206TyrAsn: 2.206 ± 0.053	1.394TyrPro: 1.394 ± 0.038	1.605TyrGln: 1.605 ± 0.041	2.007TyrArg: 2.007 ± 0.059	2.576TyrSer: 2.576 ± 0.057	2.149TyrThr: 2.149 ± 0.06	2.305TyrVal: 2.305 ± 0.049	0.484TyrTrp: 0.484 ± 0.023	1.669TyrTyr: 1.669 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2989 proteins (982028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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