American hop latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1W5T4|I1W5T4_9VIRU Movement protein TGB2 OS=American hop latent virus OX=1177630 PE=3 SV=1
MM1 pKa = 7.61EE2 pKa = 5.0IVLEE6 pKa = 4.03IAEE9 pKa = 4.28KK10 pKa = 10.33FGFVRR15 pKa = 11.84VSNKK19 pKa = 9.71LSKK22 pKa = 10.4PLVFHH27 pKa = 6.58CVPGAGKK34 pKa = 10.35SSLIRR39 pKa = 11.84EE40 pKa = 4.59LLNASDD46 pKa = 4.2GFRR49 pKa = 11.84AYY51 pKa = 9.68TFGACDD57 pKa = 3.35RR58 pKa = 11.84VNLSGKK64 pKa = 8.78YY65 pKa = 8.82IRR67 pKa = 11.84RR68 pKa = 11.84APWSDD73 pKa = 2.93SDD75 pKa = 4.22ILGKK79 pKa = 10.6LLLVDD84 pKa = 5.22EE85 pKa = 4.59YY86 pKa = 11.54TEE88 pKa = 4.36GTFDD92 pKa = 4.34LSIAFAVFGDD102 pKa = 4.17PLQSTRR108 pKa = 11.84DD109 pKa = 3.83NFCTPHH115 pKa = 5.53FTCHH119 pKa = 6.0LSRR122 pKa = 11.84RR123 pKa = 11.84FGQKK127 pKa = 7.99TASLLRR133 pKa = 11.84LLGYY137 pKa = 10.19QIEE140 pKa = 4.57STKK143 pKa = 10.85EE144 pKa = 3.74DD145 pKa = 3.24VVLIEE150 pKa = 6.09DD151 pKa = 4.13IFKK154 pKa = 10.53ADD156 pKa = 4.21PIGEE160 pKa = 4.09IVCYY164 pKa = 10.14EE165 pKa = 3.92KK166 pKa = 10.66EE167 pKa = 4.11IYY169 pKa = 10.42NLLCAHH175 pKa = 6.33CVEE178 pKa = 5.37FKK180 pKa = 10.7TPDD183 pKa = 3.71EE184 pKa = 4.04IRR186 pKa = 11.84GATFKK191 pKa = 11.05NVTFITASSEE201 pKa = 4.16PVDD204 pKa = 3.84SKK206 pKa = 11.74AAFICLTRR214 pKa = 11.84HH215 pKa = 4.77TEE217 pKa = 3.89KK218 pKa = 11.06LQILCPDD225 pKa = 3.39ASFAPSQLHH234 pKa = 5.57

Molecular weight: 26.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1W5T7|I1W5T7_9VIRU RNA silencing suppressor OS=American hop latent virus OX=1177630 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.14GARR5 pKa = 11.84LATLCLVFNKK15 pKa = 10.57LCGTYY20 pKa = 10.92VMPVCIDD27 pKa = 3.52IIKK30 pKa = 10.37RR31 pKa = 11.84SRR33 pKa = 11.84APLLTNGTSKK43 pKa = 10.08YY44 pKa = 10.04ARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84AKK51 pKa = 9.96SVCRR55 pKa = 11.84CEE57 pKa = 3.19RR58 pKa = 11.84CYY60 pKa = 10.7RR61 pKa = 11.84VFPPLRR67 pKa = 11.84NSKK70 pKa = 10.3CDD72 pKa = 3.43NKK74 pKa = 9.78TCKK77 pKa = 9.96PGISYY82 pKa = 10.7RR83 pKa = 11.84EE84 pKa = 4.2DD85 pKa = 2.91IKK87 pKa = 11.23EE88 pKa = 3.95YY89 pKa = 10.15ILWGVTEE96 pKa = 5.21VIPQPGNCFSCRR108 pKa = 11.84KK109 pKa = 9.11NLNNII114 pKa = 3.52

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2820

66

1977

470.0

53.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.376 ± 0.578

3.05 ± 0.739

4.929 ± 0.59

7.234 ± 0.989

4.965 ± 0.639

5.922 ± 0.461

2.34 ± 0.389

5.638 ± 0.332

6.454 ± 1.103

9.716 ± 0.712

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.281

4.326 ± 0.426

4.574 ± 1.299

2.553 ± 0.336

6.241 ± 0.695

6.773 ± 1.144

4.645 ± 0.547

6.915 ± 0.494

0.957 ± 0.204

3.511 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski