Vibrio phage VspDsh_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BK75|A0A411BK75_9CAUD Uncharacterized protein OS=Vibrio phage VspDsh_1 OX=2484248 GN=VspDsh1_18 PE=4 SV=1
MM1 pKa = 6.23TTAAFDD7 pKa = 3.93GKK9 pKa = 10.9LFATDD14 pKa = 3.6KK15 pKa = 10.96QVTSNNHH22 pKa = 4.93ASDD25 pKa = 3.48RR26 pKa = 11.84FARR29 pKa = 11.84KK30 pKa = 9.6LYY32 pKa = 9.77FYY34 pKa = 10.07PDD36 pKa = 3.47YY37 pKa = 11.55GLVLTVAGNAPSFIEE52 pKa = 4.2VVDD55 pKa = 3.69WAVGEE60 pKa = 4.18IATEE64 pKa = 4.04QRR66 pKa = 11.84FSVPDD71 pKa = 3.47GDD73 pKa = 4.07FTVLAVRR80 pKa = 11.84VTPGLPNMIYY90 pKa = 9.92TGFRR94 pKa = 11.84GPDD97 pKa = 3.36NQCGHH102 pKa = 6.05MEE104 pKa = 3.98RR105 pKa = 11.84EE106 pKa = 4.2YY107 pKa = 11.08EE108 pKa = 3.93KK109 pKa = 10.21RR110 pKa = 11.84TAIGSGGQFALGAMAMKK127 pKa = 10.28GSAVEE132 pKa = 4.56AIEE135 pKa = 3.93IAMDD139 pKa = 4.11LDD141 pKa = 4.47VYY143 pKa = 10.69TGGGIDD149 pKa = 3.56WFDD152 pKa = 4.48TITMEE157 pKa = 4.93HH158 pKa = 6.27SADD161 pKa = 3.57VIRR164 pKa = 11.84RR165 pKa = 11.84TLKK168 pKa = 9.78TDD170 pKa = 2.58WPTWVPPMSEE180 pKa = 4.12PEE182 pKa = 4.0APEE185 pKa = 4.06NPEE188 pKa = 4.58DD189 pKa = 3.9EE190 pKa = 4.44QQ191 pKa = 5.0

Molecular weight: 21.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BKB5|A0A411BKB5_9CAUD Uncharacterized protein OS=Vibrio phage VspDsh_1 OX=2484248 GN=VspDsh1_50 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 9.83LASHH7 pKa = 6.32ITVDD11 pKa = 3.32GVTYY15 pKa = 10.43SVSRR19 pKa = 11.84SKK21 pKa = 10.78FGYY24 pKa = 9.22YY25 pKa = 8.38ATKK28 pKa = 10.15NGQRR32 pKa = 11.84VTGNFKK38 pKa = 10.91NLDD41 pKa = 4.01LLEE44 pKa = 4.59KK45 pKa = 10.6ALEE48 pKa = 4.08RR49 pKa = 11.84LL50 pKa = 3.71

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13855

50

842

251.9

28.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.495 ± 0.608

1.306 ± 0.168

5.789 ± 0.237

6.734 ± 0.36

3.717 ± 0.183

7.571 ± 0.278

2.158 ± 0.237

5.204 ± 0.174

6.164 ± 0.35

7.578 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.952 ± 0.161

4.619 ± 0.179

4.157 ± 0.238

4.345 ± 0.522

5.327 ± 0.224

5.428 ± 0.236

6.453 ± 0.346

6.979 ± 0.298

1.624 ± 0.154

3.399 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski