Actinomadura bangladeshensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4P7S0|A0A4R4P7S0_9ACTN HypC/HybG/HupF family hydrogenase formation chaperone OS=Actinomadura bangladeshensis OX=453573 GN=E1284_06990 PE=3 SV=1
MM1 pKa = 7.16TLSAPVLDD9 pKa = 5.14APDD12 pKa = 4.2PQALADD18 pKa = 4.66FYY20 pKa = 11.58QRR22 pKa = 11.84LLGWTVVQQHH32 pKa = 6.65ADD34 pKa = 3.31WVKK37 pKa = 10.27LRR39 pKa = 11.84PPGGGTGLSFQTEE52 pKa = 4.97PIYY55 pKa = 10.25EE56 pKa = 4.37PPTWPAAEE64 pKa = 4.2GTQQIEE70 pKa = 3.77AHH72 pKa = 6.72LDD74 pKa = 3.61FEE76 pKa = 5.04VDD78 pKa = 4.12DD79 pKa = 5.69LEE81 pKa = 4.51AACDD85 pKa = 3.35HH86 pKa = 6.79AEE88 pKa = 3.98AVGAVLADD96 pKa = 4.16FQPEE100 pKa = 3.98DD101 pKa = 3.82DD102 pKa = 3.73VRR104 pKa = 11.84VYY106 pKa = 10.94LDD108 pKa = 3.46PAGHH112 pKa = 6.95PFCLFLPEE120 pKa = 4.65PEE122 pKa = 4.5SPAA125 pKa = 3.5

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4N9T1|A0A4R4N9T1_9ACTN Foldase YidC OS=Actinomadura bangladeshensis OX=453573 GN=yidC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSGRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ARR42 pKa = 11.84IAVV45 pKa = 3.5

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7888

0

7888

2512395

29

3649

318.5

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.724 ± 0.045

0.798 ± 0.007

6.139 ± 0.021

5.687 ± 0.029

2.804 ± 0.016

9.644 ± 0.027

2.203 ± 0.012

3.477 ± 0.019

2.053 ± 0.021

10.266 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.882 ± 0.01

1.716 ± 0.014

6.166 ± 0.025

2.503 ± 0.018

8.624 ± 0.031

4.69 ± 0.019

5.617 ± 0.026

8.443 ± 0.027

1.516 ± 0.012

2.049 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski