Aquimarina spongiae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Aquimarina

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4714 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6A1S5|A0A1M6A1S5_9FLAO Arylsulfatase A OS=Aquimarina spongiae OX=570521 GN=SAMN04488508_1015 PE=3 SV=1
PP1 pKa = 6.97MPDD4 pKa = 2.7ITVVTDD10 pKa = 3.32EE11 pKa = 5.29ADD13 pKa = 3.51NCSSSPVVAFVEE25 pKa = 5.12DD26 pKa = 3.64ISDD29 pKa = 3.75GNSNPEE35 pKa = 4.22VITRR39 pKa = 11.84IYY41 pKa = 10.83SVTDD45 pKa = 3.23AAGNSINVSQTITINDD61 pKa = 3.67TTFPTASNPSSISAQCSAPMPDD83 pKa = 2.66ITVVTDD89 pKa = 3.31EE90 pKa = 5.43ADD92 pKa = 3.37NCSTPNVAFVSDD104 pKa = 4.72VITSPGIITRR114 pKa = 11.84TYY116 pKa = 10.43SVTDD120 pKa = 3.38AAEE123 pKa = 3.63NSINVTQTITLNDD136 pKa = 4.14NILPTASNLPPINAQCSAPAPDD158 pKa = 3.19ITIITDD164 pKa = 3.17HH165 pKa = 7.11TDD167 pKa = 2.88NCGTPTIAFVNDD179 pKa = 3.25VSDD182 pKa = 4.18GNSNPEE188 pKa = 4.24VITRR192 pKa = 11.84TYY194 pKa = 11.12SVTDD198 pKa = 3.4AAGNSINVTQIITINDD214 pKa = 3.62GTDD217 pKa = 3.28PNINCPANITQNADD231 pKa = 3.28ANSTFATVVYY241 pKa = 10.34SDD243 pKa = 5.23PIVNDD248 pKa = 3.17NCGVNSIVQLTGLPSGAEE266 pKa = 3.97FPIGTTINTFVLTDD280 pKa = 3.47NAGNTSQCSFEE291 pKa = 4.18VTVIDD296 pKa = 5.44NPLPTCTIDD305 pKa = 3.06AGEE308 pKa = 4.6DD309 pKa = 3.39EE310 pKa = 5.7RR311 pKa = 11.84ITEE314 pKa = 4.12GEE316 pKa = 4.35EE317 pKa = 3.66IQLDD321 pKa = 3.83ATASTTGSFVWSPSIGLSDD340 pKa = 3.58TTTSNPIASPSITTTYY356 pKa = 10.48LVEE359 pKa = 4.28FTSEE363 pKa = 4.12DD364 pKa = 3.36GCVAVDD370 pKa = 3.47EE371 pKa = 5.14VIVFVTPQEE380 pKa = 4.08EE381 pKa = 4.35DD382 pKa = 2.87EE383 pKa = 4.32TRR385 pKa = 11.84YY386 pKa = 10.68GFSPDD391 pKa = 2.99GDD393 pKa = 4.16GINEE397 pKa = 3.9FWEE400 pKa = 3.89IDD402 pKa = 3.7TIEE405 pKa = 4.46NYY407 pKa = 10.0PNNSVSIFNRR417 pKa = 11.84WGDD420 pKa = 3.53LVFEE424 pKa = 4.26IEE426 pKa = 4.78GYY428 pKa = 10.9NNTSRR433 pKa = 11.84VFRR436 pKa = 11.84GIANRR441 pKa = 11.84KK442 pKa = 8.65RR443 pKa = 11.84SLGGDD448 pKa = 3.34QLPEE452 pKa = 3.48GTYY455 pKa = 9.89FFKK458 pKa = 10.67IRR460 pKa = 11.84TSGPNSLRR468 pKa = 11.84KK469 pKa = 8.35TEE471 pKa = 4.14GFLVLKK477 pKa = 10.6RR478 pKa = 3.84

Molecular weight: 50.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6EC64|A0A1M6EC64_9FLAO Elongation factor Ts OS=Aquimarina spongiae OX=570521 GN=tsf PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.59SKK4 pKa = 9.01ITLILIICLATTHH17 pKa = 7.14LFAQEE22 pKa = 4.54DD23 pKa = 3.94LKK25 pKa = 11.52LWTGYY30 pKa = 10.23SAGARR35 pKa = 11.84ITKK38 pKa = 7.99EE39 pKa = 3.57WRR41 pKa = 11.84VSAGQLLLFSQQPFEE56 pKa = 4.29LSSMQNSVNLTYY68 pKa = 10.76RR69 pKa = 11.84FDD71 pKa = 3.45RR72 pKa = 11.84RR73 pKa = 11.84FRR75 pKa = 11.84AGFGYY80 pKa = 10.35LRR82 pKa = 11.84SSDD85 pKa = 3.93PSDD88 pKa = 3.95PDD90 pKa = 3.0QEE92 pKa = 3.98ARR94 pKa = 11.84NRR96 pKa = 11.84VTGRR100 pKa = 11.84FRR102 pKa = 11.84YY103 pKa = 9.1NARR106 pKa = 11.84LGKK109 pKa = 10.0FRR111 pKa = 11.84FLNSLRR117 pKa = 11.84AEE119 pKa = 3.69WHH121 pKa = 5.51FPEE124 pKa = 4.94RR125 pKa = 11.84SKK127 pKa = 11.26FEE129 pKa = 3.41YY130 pKa = 10.06RR131 pKa = 11.84IRR133 pKa = 11.84YY134 pKa = 7.84GIRR137 pKa = 11.84IHH139 pKa = 6.49RR140 pKa = 11.84GSWGLPLRR148 pKa = 11.84TTPFITNEE156 pKa = 3.27FHH158 pKa = 7.04YY159 pKa = 9.69YY160 pKa = 10.4LSGRR164 pKa = 11.84PLQYY168 pKa = 9.87RR169 pKa = 11.84DD170 pKa = 3.44EE171 pKa = 4.3QGEE174 pKa = 4.45KK175 pKa = 10.33VVKK178 pKa = 10.27QSPDD182 pKa = 2.78GLHH185 pKa = 6.03AHH187 pKa = 7.73RR188 pKa = 11.84ITLGVRR194 pKa = 11.84FRR196 pKa = 11.84PFKK199 pKa = 10.36RR200 pKa = 11.84ANASISYY207 pKa = 8.32MRR209 pKa = 11.84QTEE212 pKa = 3.93FNIGSKK218 pKa = 9.99YY219 pKa = 10.35RR220 pKa = 11.84EE221 pKa = 4.16INVTDD226 pKa = 4.14PRR228 pKa = 11.84DD229 pKa = 3.56GDD231 pKa = 3.53ILRR234 pKa = 11.84AFNNFSVLMFRR245 pKa = 11.84FSYY248 pKa = 10.67RR249 pKa = 11.84LDD251 pKa = 3.23FRR253 pKa = 11.84RR254 pKa = 11.84RR255 pKa = 11.84TLRR258 pKa = 11.84PNAIGFHH265 pKa = 5.38TKK267 pKa = 8.6KK268 pKa = 10.28QKK270 pKa = 10.25PIPHH274 pKa = 6.2SEE276 pKa = 4.1EE277 pKa = 3.9SHH279 pKa = 5.65TSII282 pKa = 5.37

Molecular weight: 33.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4714

0

4714

1614774

39

5059

342.5

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.114 ± 0.032

0.745 ± 0.012

5.816 ± 0.034

6.633 ± 0.043

5.226 ± 0.03

6.492 ± 0.043

1.837 ± 0.017

7.889 ± 0.032

7.348 ± 0.056

9.097 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.019

6.009 ± 0.037

3.447 ± 0.027

3.73 ± 0.023

3.593 ± 0.024

6.473 ± 0.029

6.016 ± 0.047

6.24 ± 0.027

1.101 ± 0.014

4.12 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski