Vibrio sp. qd031

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1MNI0|A0A1X1MNI0_9VIBR DUF4115 domain-containing protein OS=Vibrio sp. qd031 OX=1603038 GN=ST37_16220 PE=4 SV=1
MM1 pKa = 7.59FKK3 pKa = 8.36TTLFTALGCMFIAGQAQAITFQFDD27 pKa = 3.63WNGGAYY33 pKa = 10.08DD34 pKa = 3.79YY35 pKa = 10.81AIPNGGAAANGNDD48 pKa = 3.56YY49 pKa = 11.48DD50 pKa = 4.32GVEE53 pKa = 4.09LVYY56 pKa = 10.68DD57 pKa = 4.13YY58 pKa = 11.53DD59 pKa = 4.05VSDD62 pKa = 4.0YY63 pKa = 10.46LTVGSSTGDD72 pKa = 3.39TVQDD76 pKa = 3.58ISPPNAGIGADD87 pKa = 3.34GGVNGDD93 pKa = 3.34NTGIGEE99 pKa = 4.44WLTLAFSSDD108 pKa = 3.57VTVTAISFNGGTGTPGAGHH127 pKa = 7.31GDD129 pKa = 3.22TWDD132 pKa = 3.95GDD134 pKa = 3.68SHH136 pKa = 7.05EE137 pKa = 4.72ILFEE141 pKa = 4.08LVNGDD146 pKa = 2.9TDD148 pKa = 4.68AIEE151 pKa = 4.74DD152 pKa = 4.04SKK154 pKa = 9.9THH156 pKa = 5.96NFAGSPDD163 pKa = 3.58GVLDD167 pKa = 3.91GLTGDD172 pKa = 3.96VLTDD176 pKa = 3.34DD177 pKa = 4.97FVRR180 pKa = 11.84ITSVDD185 pKa = 2.85TRR187 pKa = 11.84FYY189 pKa = 11.01LEE191 pKa = 4.79SITFEE196 pKa = 4.23LSDD199 pKa = 4.36IEE201 pKa = 4.19ITTFGIPVPEE211 pKa = 4.91PEE213 pKa = 4.21TLVLFSLGLIGLGASLRR230 pKa = 11.84HH231 pKa = 5.85RR232 pKa = 11.84RR233 pKa = 11.84RR234 pKa = 11.84TSYY237 pKa = 10.96SS238 pKa = 2.96

Molecular weight: 25.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1MVN7|A0A1X1MVN7_9VIBR Uncharacterized protein OS=Vibrio sp. qd031 OX=1603038 GN=ST37_05600 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LSKK44 pKa = 10.83

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3553

0

3553

1144937

36

2389

322.2

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.596 ± 0.046

1.016 ± 0.014

5.688 ± 0.038

6.223 ± 0.04

4.112 ± 0.026

6.834 ± 0.031

2.364 ± 0.022

6.297 ± 0.029

5.058 ± 0.036

10.263 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.02

4.106 ± 0.022

3.884 ± 0.024

4.668 ± 0.031

4.481 ± 0.028

6.747 ± 0.036

5.479 ± 0.034

7.214 ± 0.035

1.271 ± 0.018

3.047 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski