Cotton leaf curl Burewala virus - [India:Vehari:2006]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Cotton leaf curl Kokhran virus

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7ZEN8|B7ZEN8_9GEMI C4 protein OS=Cotton leaf curl Burewala virus - [India:Vehari:2006] OX=418448 GN=C4 PE=3 SV=1
MM1 pKa = 7.7APPKK5 pKa = 10.14RR6 pKa = 11.84FKK8 pKa = 10.93VQAKK12 pKa = 9.81NYY14 pKa = 9.23FITYY18 pKa = 6.67PQCSLTKK25 pKa = 10.54EE26 pKa = 4.03EE27 pKa = 5.09ALSQIQAINTPSNKK41 pKa = 9.74KK42 pKa = 9.85YY43 pKa = 10.49IKK45 pKa = 10.16LCRR48 pKa = 11.84EE49 pKa = 3.86LHH51 pKa = 6.34EE52 pKa = 5.81DD53 pKa = 4.2GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.8GKK67 pKa = 9.67FVCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.45SSSDD99 pKa = 3.03VKK101 pKa = 11.13AYY103 pKa = 9.68IDD105 pKa = 3.75KK106 pKa = 11.33DD107 pKa = 3.43GDD109 pKa = 3.69TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.32GRR121 pKa = 11.84SAGGGQQTANDD132 pKa = 4.05AYY134 pKa = 10.69AAALNAGSKK143 pKa = 10.29SEE145 pKa = 3.84ALRR148 pKa = 11.84VIKK151 pKa = 10.2EE152 pKa = 3.88LAPKK156 pKa = 10.6DD157 pKa = 3.7FVLQFHH163 pKa = 6.7NLNANLDD170 pKa = 4.29RR171 pKa = 11.84IFQEE175 pKa = 4.33PPAPYY180 pKa = 9.43ISPFSPSSFDD190 pKa = 3.4QVPEE194 pKa = 3.95EE195 pKa = 4.36LEE197 pKa = 3.76VWAIDD202 pKa = 3.86NVVDD206 pKa = 4.22AAARR210 pKa = 11.84PLRR213 pKa = 11.84PRR215 pKa = 11.84SIVIEE220 pKa = 4.26GDD222 pKa = 3.21SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 4.79DD262 pKa = 4.33VDD264 pKa = 3.73PHH266 pKa = 6.08FLKK269 pKa = 10.67HH270 pKa = 5.9FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.59NEE313 pKa = 3.77FLNEE317 pKa = 4.26GKK319 pKa = 8.27NTSLKK324 pKa = 10.45NWALKK329 pKa = 10.24NATFITLEE337 pKa = 4.3GPLYY341 pKa = 10.49SASNQSTAQGSEE353 pKa = 3.84EE354 pKa = 4.11TQQEE358 pKa = 4.32EE359 pKa = 4.54EE360 pKa = 4.33SRR362 pKa = 11.84SS363 pKa = 3.6

Molecular weight: 40.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7ZEN7|B7ZEN7_9GEMI Replication-associated protein OS=Cotton leaf curl Burewala virus - [India:Vehari:2006] OX=418448 GN=Rep PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 4.53SRR4 pKa = 11.84TGEE7 pKa = 4.67PITAAQAGNGAYY19 pKa = 9.06IWEE22 pKa = 4.6VPNPLYY28 pKa = 10.55FKK30 pKa = 10.41IISYY34 pKa = 8.72VNRR37 pKa = 11.84PFTTNMDD44 pKa = 3.11ILMIRR49 pKa = 11.84IQFNHH54 pKa = 6.33NLRR57 pKa = 11.84KK58 pKa = 9.8ALGIHH63 pKa = 6.03KK64 pKa = 9.67CFIAFRR70 pKa = 11.84IWMTSQPPTGRR81 pKa = 11.84FLRR84 pKa = 11.84VFRR87 pKa = 11.84TQVLKK92 pKa = 10.96YY93 pKa = 10.02LNNLGIVSINNVVRR107 pKa = 11.84AVDD110 pKa = 3.53HH111 pKa = 5.92VLWNVLQHH119 pKa = 6.03VVFVDD124 pKa = 3.54QSSSIKK130 pKa = 10.4FNIYY134 pKa = 10.21

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1052

118

363

210.4

24.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.369 ± 0.724

1.901 ± 0.578

4.563 ± 0.514

4.563 ± 0.766

4.468 ± 0.514

4.563 ± 0.618

3.232 ± 0.367

5.703 ± 0.85

6.084 ± 0.705

6.654 ± 0.89

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.376 ± 0.604

5.989 ± 0.702

6.274 ± 0.495

5.323 ± 0.797

6.559 ± 1.073

8.65 ± 1.51

5.323 ± 0.502

6.274 ± 1.461

1.426 ± 0.17

3.707 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski