Pseudoalteromonas phage PH357

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5PTG8|A0A1W5PTG8_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PH357 OX=1913046 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84NLSAEE7 pKa = 4.33LIKK10 pKa = 10.87LQNTLINTKK19 pKa = 10.27SEE21 pKa = 4.02LKK23 pKa = 9.96EE24 pKa = 3.87AQNLLDD30 pKa = 4.81HH31 pKa = 6.59MEE33 pKa = 4.57SDD35 pKa = 3.96PAAHH39 pKa = 6.85FEE41 pKa = 4.34DD42 pKa = 4.71EE43 pKa = 4.49MKK45 pKa = 10.68EE46 pKa = 4.38SYY48 pKa = 11.0NQMLDD53 pKa = 2.99EE54 pKa = 5.28CYY56 pKa = 10.7SEE58 pKa = 5.7AFDD61 pKa = 4.9SFPFNLGSPSEE72 pKa = 4.34WIEE75 pKa = 3.6EE76 pKa = 4.11NQPVDD81 pKa = 3.58YY82 pKa = 10.52RR83 pKa = 11.84VGFSDD88 pKa = 4.47YY89 pKa = 11.43DD90 pKa = 3.57FDD92 pKa = 4.48YY93 pKa = 11.38SNVSEE98 pKa = 4.24YY99 pKa = 11.22VEE101 pKa = 3.96QQEE104 pKa = 5.67LVDD107 pKa = 3.71QLEE110 pKa = 4.54SEE112 pKa = 4.6VEE114 pKa = 3.89DD115 pKa = 3.99LEE117 pKa = 5.32AEE119 pKa = 4.1IEE121 pKa = 4.07EE122 pKa = 4.59LQTGIDD128 pKa = 3.7EE129 pKa = 4.71IEE131 pKa = 4.14NNN133 pKa = 3.72

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5PTH0|A0A1W5PTH0_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage PH357 OX=1913046 PE=4 SV=1
MM1 pKa = 7.28QSQNITKK8 pKa = 10.18IKK10 pKa = 9.38RR11 pKa = 11.84TQKK14 pKa = 10.2PALKK18 pKa = 8.73TANIYY23 pKa = 10.68EE24 pKa = 4.32SDD26 pKa = 4.12SKK28 pKa = 11.15RR29 pKa = 11.84KK30 pKa = 9.55AIKK33 pKa = 8.57QARR36 pKa = 11.84RR37 pKa = 11.84HH38 pKa = 5.29KK39 pKa = 10.64RR40 pKa = 11.84IAQEE44 pKa = 3.61LNYY47 pKa = 10.98

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

242

0

242

38862

37

1162

160.6

18.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.712 ± 0.206

1.83 ± 0.114

7.02 ± 0.134

8.695 ± 0.201

4.426 ± 0.093

5.867 ± 0.188

1.788 ± 0.074

6.477 ± 0.133

8.517 ± 0.265

8.26 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.089

5.437 ± 0.147

2.838 ± 0.108

3.147 ± 0.117

3.837 ± 0.107

6.557 ± 0.137

5.218 ± 0.181

6.688 ± 0.146

1.384 ± 0.058

4.948 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski