bacterium HR07

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5VIG4|A0A2H5VIG4_9BACT 3-ketoacyl-CoA thiolase OS=bacterium HR07 OX=2035402 GN=fadA PE=3 SV=1
MM1 pKa = 7.83PEE3 pKa = 3.58VTIYY7 pKa = 7.52TTPTCSWCAAAKK19 pKa = 10.27RR20 pKa = 11.84FLDD23 pKa = 3.49EE24 pKa = 5.11HH25 pKa = 6.53EE26 pKa = 4.37IDD28 pKa = 3.58YY29 pKa = 10.56TEE31 pKa = 4.61YY32 pKa = 10.9DD33 pKa = 3.39VSEE36 pKa = 4.63DD37 pKa = 3.74PEE39 pKa = 4.25VLLRR43 pKa = 11.84LSGQTGVPVLDD54 pKa = 3.43IDD56 pKa = 4.11GEE58 pKa = 4.46IVVGFDD64 pKa = 2.9RR65 pKa = 11.84GRR67 pKa = 11.84IAEE70 pKa = 4.17LLGLEE75 pKa = 4.81DD76 pKa = 3.71EE77 pKa = 4.77

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5VHC6|A0A2H5VHC6_9BACT Protein TolB OS=bacterium HR07 OX=2035402 GN=tolB_1 PE=4 SV=1
MM1 pKa = 7.24SASPASPIRR10 pKa = 11.84KK11 pKa = 7.62TGQKK15 pKa = 9.86SAWFIVKK22 pKa = 10.29KK23 pKa = 10.94LMGSLKK29 pKa = 10.09TSIPNAKK36 pKa = 9.98VMITAMVPVRR46 pKa = 11.84VRR48 pKa = 11.84ATPRR52 pKa = 11.84PNRR55 pKa = 3.63

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

726

0

726

183638

31

1558

252.9

28.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.336 ± 0.117

0.992 ± 0.043

4.539 ± 0.07

7.18 ± 0.105

4.028 ± 0.07

7.68 ± 0.091

2.252 ± 0.056

6.097 ± 0.08

4.549 ± 0.079

11.171 ± 0.115

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.904 ± 0.044

2.348 ± 0.051

5.155 ± 0.072

3.969 ± 0.079

7.048 ± 0.093

4.88 ± 0.068

5.086 ± 0.072

7.737 ± 0.083

1.307 ± 0.037

2.741 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski