Pontimonas phage phiPsal1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VGQ9|A0A2K9VGQ9_9CAUD Uncharacterized protein OS=Pontimonas phage phiPsal1 OX=2079347 GN=phiPsal1_046 PE=4 SV=1
MM1 pKa = 7.01GHH3 pKa = 5.96WAKK6 pKa = 10.52IEE8 pKa = 4.28DD9 pKa = 3.73NRR11 pKa = 11.84VVQVVVADD19 pKa = 4.14DD20 pKa = 5.69DD21 pKa = 4.31KK22 pKa = 11.96GDD24 pKa = 3.66WLAEE28 pKa = 3.99ALGGTWIQTSYY39 pKa = 8.4NTRR42 pKa = 11.84GGVHH46 pKa = 5.26YY47 pKa = 9.85TDD49 pKa = 5.27GEE51 pKa = 4.16PSADD55 pKa = 3.16QSKK58 pKa = 10.78ALRR61 pKa = 11.84GNYY64 pKa = 9.8AGVGFTYY71 pKa = 10.72DD72 pKa = 3.27ADD74 pKa = 4.01RR75 pKa = 11.84DD76 pKa = 3.83AFIPPKK82 pKa = 10.56PFDD85 pKa = 3.3SWVLDD90 pKa = 3.56EE91 pKa = 5.49ATCLWTAPIPYY102 pKa = 9.03PADD105 pKa = 3.58GGQYY109 pKa = 10.12VWDD112 pKa = 3.98EE113 pKa = 4.16ATTDD117 pKa = 3.0WLEE120 pKa = 4.16VVDD123 pKa = 5.08EE124 pKa = 4.37VLL126 pKa = 4.57

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VGT2|A0A2K9VGT2_9CAUD DNA methylase OS=Pontimonas phage phiPsal1 OX=2079347 GN=phiPsal1_045 PE=3 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84FYY4 pKa = 11.25NPWPEE9 pKa = 3.9PYY11 pKa = 9.82RR12 pKa = 11.84INARR16 pKa = 11.84SPFGPRR22 pKa = 11.84RR23 pKa = 11.84HH24 pKa = 6.88PITGKK29 pKa = 9.97QSFHH33 pKa = 7.15HH34 pKa = 6.57GVDD37 pKa = 3.64VACPVGTPLIAGADD51 pKa = 3.57GAVAHH56 pKa = 6.94KK57 pKa = 11.04GNGASGGHH65 pKa = 4.96VLILRR70 pKa = 11.84HH71 pKa = 6.28AGNFHH76 pKa = 5.38TVYY79 pKa = 10.67YY80 pKa = 9.79HH81 pKa = 7.04LKK83 pKa = 9.99EE84 pKa = 4.2PSHH87 pKa = 6.76LNLGEE92 pKa = 3.93PVKK95 pKa = 11.06AGDD98 pKa = 3.52IVALSGNTGASTGPHH113 pKa = 5.32LHH115 pKa = 6.07FEE117 pKa = 4.48VRR119 pKa = 11.84RR120 pKa = 11.84SRR122 pKa = 11.84KK123 pKa = 8.97WGDD126 pKa = 3.58TIDD129 pKa = 4.15PQPLLKK135 pKa = 10.1GTFRR139 pKa = 11.84EE140 pKa = 4.53APQAPSRR147 pKa = 11.84SQRR150 pKa = 11.84PSRR153 pKa = 11.84LGRR156 pKa = 11.84VTPGLEE162 pKa = 3.77ALSNSWIARR171 pKa = 11.84GAHH174 pKa = 7.04AIRR177 pKa = 11.84RR178 pKa = 11.84GLGRR182 pKa = 3.89

Molecular weight: 19.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

9129

37

740

163.0

17.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.618 ± 0.472

0.592 ± 0.108

6.331 ± 0.334

7.011 ± 0.445

4.174 ± 0.235

8.216 ± 0.359

1.895 ± 0.215

4.743 ± 0.349

4.184 ± 0.262

8.643 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.171

3.681 ± 0.238

4.601 ± 0.332

3.483 ± 0.254

6.145 ± 0.325

5.762 ± 0.307

6.014 ± 0.279

8.435 ± 0.511

1.84 ± 0.196

2.18 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski