Marinilactibacillus sp. 15R

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Marinilactibacillus; unclassified Marinilactibacillus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2486 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4BZL0|A0A1L4BZL0_9LACT Dihydroorotase OS=Marinilactibacillus sp. 15R OX=1911586 GN=pyrC PE=3 SV=1
MM1 pKa = 7.45SNKK4 pKa = 9.73KK5 pKa = 10.09KK6 pKa = 10.73YY7 pKa = 10.59SLLASSAAVAFVLAACGGSDD27 pKa = 5.14NADD30 pKa = 3.45SDD32 pKa = 4.4NGSSDD37 pKa = 3.3NGGSEE42 pKa = 4.26SGSEE46 pKa = 4.23GPLTFEE52 pKa = 3.97TRR54 pKa = 11.84VEE56 pKa = 4.18NEE58 pKa = 3.77GEE60 pKa = 4.21AIEE63 pKa = 4.8GGTMNIGYY71 pKa = 10.2VSDD74 pKa = 3.98SPFTGIFNWEE84 pKa = 4.33LYY86 pKa = 10.46DD87 pKa = 4.22GNPDD91 pKa = 3.71AQVMAYY97 pKa = 9.54TMGEE101 pKa = 3.97LVGSDD106 pKa = 3.5EE107 pKa = 4.94NYY109 pKa = 10.71QIDD112 pKa = 4.06DD113 pKa = 3.67SGAATLEE120 pKa = 4.02LDD122 pKa = 3.6QEE124 pKa = 4.74ANTVTITVKK133 pKa = 10.87DD134 pKa = 4.03GIKK137 pKa = 9.22WHH139 pKa = 7.12DD140 pKa = 4.17GEE142 pKa = 5.31PLTADD147 pKa = 3.44DD148 pKa = 4.55VLFAHH153 pKa = 7.28EE154 pKa = 4.83IIGDD158 pKa = 3.71PEE160 pKa = 4.33YY161 pKa = 11.02TGVRR165 pKa = 11.84YY166 pKa = 7.47TADD169 pKa = 3.31LQNIVGMEE177 pKa = 3.97EE178 pKa = 4.35YY179 pKa = 10.84KK180 pKa = 11.01NGEE183 pKa = 3.99ADD185 pKa = 4.03SISGMTLSDD194 pKa = 4.54DD195 pKa = 3.88EE196 pKa = 4.47MTLTIQYY203 pKa = 10.33KK204 pKa = 9.75SVGVQMLQAGGGVWTYY220 pKa = 11.67AEE222 pKa = 4.14PRR224 pKa = 11.84HH225 pKa = 5.53YY226 pKa = 11.05LGDD229 pKa = 3.6IPVADD234 pKa = 4.76LEE236 pKa = 4.52SAPEE240 pKa = 4.01VRR242 pKa = 11.84EE243 pKa = 3.94KK244 pKa = 10.85PLGYY248 pKa = 10.89GPFKK252 pKa = 10.65VDD254 pKa = 3.48NIVPGEE260 pKa = 4.19SVSYY264 pKa = 11.15SRR266 pKa = 11.84FDD268 pKa = 3.78DD269 pKa = 3.71YY270 pKa = 11.6WRR272 pKa = 11.84GTPILDD278 pKa = 4.45GIEE281 pKa = 4.17LQTVPTATAVAAMEE295 pKa = 4.16NAQFDD300 pKa = 4.61LFIDD304 pKa = 4.09MPTDD308 pKa = 3.3QYY310 pKa = 11.75DD311 pKa = 3.59TFADD315 pKa = 3.9LPGYY319 pKa = 10.17SIVGRR324 pKa = 11.84DD325 pKa = 3.69EE326 pKa = 4.3NSYY329 pKa = 9.83TYY331 pKa = 10.35IGFKK335 pKa = 10.31LGEE338 pKa = 4.01WQEE341 pKa = 3.98AQEE344 pKa = 4.49DD345 pKa = 4.17DD346 pKa = 4.12EE347 pKa = 5.86GNITEE352 pKa = 4.3PAKK355 pKa = 10.72NVYY358 pKa = 10.42NPDD361 pKa = 2.81AKK363 pKa = 10.52MADD366 pKa = 3.42KK367 pKa = 10.86NLRR370 pKa = 11.84QAMAYY375 pKa = 10.12AIDD378 pKa = 3.78NDD380 pKa = 4.01AVGEE384 pKa = 4.04RR385 pKa = 11.84FYY387 pKa = 11.23QGLRR391 pKa = 11.84RR392 pKa = 11.84RR393 pKa = 11.84ANSPIIPNFSDD404 pKa = 4.28YY405 pKa = 11.47YY406 pKa = 10.19NEE408 pKa = 4.65DD409 pKa = 3.61VVGYY413 pKa = 9.22PYY415 pKa = 11.04DD416 pKa = 3.77EE417 pKa = 5.73DD418 pKa = 3.65EE419 pKa = 4.82ANRR422 pKa = 11.84ILDD425 pKa = 3.51EE426 pKa = 5.55AGYY429 pKa = 9.65EE430 pKa = 3.99WAEE433 pKa = 4.17GEE435 pKa = 4.41DD436 pKa = 4.21FRR438 pKa = 11.84TTPEE442 pKa = 4.08GEE444 pKa = 3.76EE445 pKa = 3.88LVINFASMSGGDD457 pKa = 3.54VAEE460 pKa = 5.66PIAEE464 pKa = 4.17YY465 pKa = 10.69YY466 pKa = 7.98IQSWARR472 pKa = 11.84IGLNVQLLEE481 pKa = 4.44GRR483 pKa = 11.84LHH485 pKa = 6.47EE486 pKa = 4.93FNSFYY491 pKa = 11.31DD492 pKa = 3.47RR493 pKa = 11.84VEE495 pKa = 4.92ADD497 pKa = 4.09DD498 pKa = 4.21PAIDD502 pKa = 4.13VYY504 pKa = 10.97QGAWGTGTDD513 pKa = 3.78PTPEE517 pKa = 3.87GLYY520 pKa = 11.14GEE522 pKa = 4.45TAPFNYY528 pKa = 8.66TRR530 pKa = 11.84WVTEE534 pKa = 3.7EE535 pKa = 3.62NNEE538 pKa = 3.9YY539 pKa = 9.2MEE541 pKa = 4.44QMLSSEE547 pKa = 4.4GFDD550 pKa = 3.6TEE552 pKa = 3.53WRR554 pKa = 11.84AEE556 pKa = 4.18VFNEE560 pKa = 3.42WQEE563 pKa = 4.2YY564 pKa = 9.52FMEE567 pKa = 4.71EE568 pKa = 4.2LPIIPTLFRR577 pKa = 11.84TEE579 pKa = 4.04VFPVNDD585 pKa = 3.27RR586 pKa = 11.84VGAFDD591 pKa = 3.2ITVGVDD597 pKa = 3.15PEE599 pKa = 4.31TAGAEE604 pKa = 4.29TGYY607 pKa = 8.56HH608 pKa = 4.1TWYY611 pKa = 8.88LTSEE615 pKa = 4.18EE616 pKa = 4.72RR617 pKa = 11.84VTEE620 pKa = 4.11

Molecular weight: 68.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4BWW1|A0A1L4BWW1_9LACT DNA starvation/stationary phase protection protein OS=Marinilactibacillus sp. 15R OX=1911586 GN=BKP56_03040 PE=3 SV=1
MM1 pKa = 7.12SQQGLTYY8 pKa = 10.09QPKK11 pKa = 8.71KK12 pKa = 10.19RR13 pKa = 11.84KK14 pKa = 7.43RR15 pKa = 11.84QKK17 pKa = 8.87VHH19 pKa = 5.8GFRR22 pKa = 11.84KK23 pKa = 10.04RR24 pKa = 11.84MSTKK28 pKa = 9.72NGRR31 pKa = 11.84RR32 pKa = 11.84VLKK35 pKa = 10.17ARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.51GRR42 pKa = 11.84KK43 pKa = 9.2RR44 pKa = 11.84ISAA47 pKa = 3.72

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2486

0

2486

740988

37

1529

298.1

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.532 ± 0.048

0.505 ± 0.012

5.532 ± 0.041

8.086 ± 0.057

4.34 ± 0.035

6.454 ± 0.048

1.846 ± 0.022

8.087 ± 0.05

7.064 ± 0.054

9.606 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.021

5.008 ± 0.032

3.245 ± 0.025

3.793 ± 0.035

3.891 ± 0.032

6.405 ± 0.036

5.667 ± 0.037

6.666 ± 0.04

0.884 ± 0.017

3.736 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski