Proteome-pI 2.0 - Proteome Isoelectric Point Database

Ruminococcaceae bacterium CPB6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1W6WCT2|A0A1W6WCT2_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium CPB6 OX=1572656 GN=B6259_03680 PE=4 SV=1

MM1 pKa = 7.65SYY3 pKa = 11.55DD4 pKa = 4.74DD5 pKa = 4.0IVTMLEE11 pKa = 4.04EE12 pKa = 4.89ADD14 pKa = 4.51LPIAYY19 pKa = 9.81DD20 pKa = 3.57HH21 pKa = 6.31FAEE24 pKa = 5.37GEE26 pKa = 4.37SPDD29 pKa = 3.74PPFICFLFPGTDD41 pKa = 3.38NMFADD46 pKa = 3.57NVVWEE51 pKa = 5.36RR52 pKa = 11.84IDD54 pKa = 3.42EE55 pKa = 4.39LNIEE59 pKa = 4.87LYY61 pKa = 9.83TDD63 pKa = 3.68KK64 pKa = 10.74KK65 pKa = 11.23DD66 pKa = 3.52PDD68 pKa = 3.77IEE70 pKa = 4.62SKK72 pKa = 10.68IEE74 pKa = 4.83DD75 pKa = 3.61ILTAHH80 pKa = 7.15EE81 pKa = 4.33LPYY84 pKa = 10.6EE85 pKa = 4.22KK86 pKa = 10.71SEE88 pKa = 3.9VWIEE92 pKa = 3.87DD93 pKa = 3.01EE94 pKa = 4.89KK95 pKa = 10.86MYY97 pKa = 10.98EE98 pKa = 3.93VLYY101 pKa = 8.62QTQILGGG108 pKa = 3.67

Molecular weight: 12.6 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1W6WDK4|A0A1W6WDK4_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium CPB6 OX=1572656 GN=B6259_04630 PE=4 SV=1

MM1 pKa = 7.9VGEE4 pKa = 4.37AFFMKK9 pKa = 10.16TSRR12 pKa = 11.84TIALKK17 pKa = 10.46PGTLLFRR24 pKa = 11.84SILTGTVGGAAICALLLLLAAAAVQSSGQLPQDD57 pKa = 4.12LLQPMILIISAVSAFFAGLFAGKK80 pKa = 9.79VSHH83 pKa = 7.14RR84 pKa = 11.84RR85 pKa = 11.84GLLLGMGCGMLLFLLCLIGGAVNSHH110 pKa = 6.96DD111 pKa = 3.83VLSISSLTRR120 pKa = 11.84MMVMVLSGALGGYY133 pKa = 8.74LAIYY137 pKa = 9.55RR138 pKa = 11.84RR139 pKa = 11.84SKK141 pKa = 10.34IRR143 pKa = 3.25

Molecular weight: 14.97 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1921	0	1921	588157	37	1701	306.2	33.85

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.048 ± 0.074	1.827 ± 0.025	5.393 ± 0.05	5.886 ± 0.06	3.855 ± 0.042	7.439 ± 0.053	2.112 ± 0.025	5.915 ± 0.051	5.859 ± 0.047	9.48 ± 0.072

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.849 ± 0.025	3.746 ± 0.039	4.161 ± 0.039	4.014 ± 0.041	5.15 ± 0.054	6.191 ± 0.06	5.768 ± 0.061	7.018 ± 0.047	0.92 ± 0.019	3.368 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski