Leptothrix mobilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Leptothrix

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7LHG7|A0A4Q7LHG7_9BURK Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE OS=Leptothrix mobilis OX=47994 GN=ubiE PE=3 SV=1
MM1 pKa = 6.82NTHH4 pKa = 7.22ILRR7 pKa = 11.84SCAAAALASACLLLSACGDD26 pKa = 3.59SSTLPDD32 pKa = 3.62VPVATAEE39 pKa = 4.08TSPAVAINLVQDD51 pKa = 3.78VAATSDD57 pKa = 3.55AQSDD61 pKa = 3.88SSEE64 pKa = 4.08PLAVPDD70 pKa = 4.08TLAVDD75 pKa = 4.35DD76 pKa = 4.51SGEE79 pKa = 3.89PRR81 pKa = 11.84AVNN84 pKa = 3.28

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7LIA8|A0A4Q7LIA8_9BURK Anhydro-N-acetylmuramic acid kinase OS=Leptothrix mobilis OX=47994 GN=anmK PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.35TAGGRR28 pKa = 11.84KK29 pKa = 8.95VIANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.36GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4025

0

4025

1415505

30

5811

351.7

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.555 ± 0.059

0.888 ± 0.014

5.729 ± 0.037

4.937 ± 0.031

2.968 ± 0.025

8.534 ± 0.039

2.436 ± 0.024

3.974 ± 0.028

2.509 ± 0.032

11.196 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.019

2.262 ± 0.028

5.582 ± 0.036

3.947 ± 0.028

7.589 ± 0.053

5.21 ± 0.03

5.238 ± 0.048

7.753 ± 0.034

1.566 ± 0.019

1.909 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski