Amino acid dipepetide frequency for Thermobifida fusca (strain YX)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.329AlaAla: 19.329 ± 0.258	0.998AlaCys: 0.998 ± 0.036	7.676AlaAsp: 7.676 ± 0.109	9.591AlaGlu: 9.591 ± 0.134	3.305AlaPhe: 3.305 ± 0.067	9.806AlaGly: 9.806 ± 0.117	2.783AlaHis: 2.783 ± 0.058	4.311AlaIle: 4.311 ± 0.072	2.528AlaLys: 2.528 ± 0.058	13.718AlaLeu: 13.718 ± 0.148	2.293AlaMet: 2.293 ± 0.045	2.078AlaAsn: 2.078 ± 0.055	6.109AlaPro: 6.109 ± 0.094	4.103AlaGln: 4.103 ± 0.072	9.623AlaArg: 9.623 ± 0.121	5.615AlaSer: 5.615 ± 0.074	6.621AlaThr: 6.621 ± 0.092	11.388AlaVal: 11.388 ± 0.151	1.725AlaTrp: 1.725 ± 0.043	2.423AlaTyr: 2.423 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.073CysAla: 1.073 ± 0.036	0.111CysCys: 0.111 ± 0.01	0.482CysAsp: 0.482 ± 0.025	0.414CysGlu: 0.414 ± 0.021	0.249CysPhe: 0.249 ± 0.018	0.917CysGly: 0.917 ± 0.032	0.197CysHis: 0.197 ± 0.014	0.2CysIle: 0.2 ± 0.016	0.094CysLys: 0.094 ± 0.009	0.723CysLeu: 0.723 ± 0.031	0.137CysMet: 0.137 ± 0.012	0.115CysAsn: 0.115 ± 0.01	0.518CysPro: 0.518 ± 0.023	0.212CysGln: 0.212 ± 0.014	0.683CysArg: 0.683 ± 0.027	0.459CysSer: 0.459 ± 0.021	0.493CysThr: 0.493 ± 0.021	0.682CysVal: 0.682 ± 0.032	0.096CysTrp: 0.096 ± 0.01	0.18CysTyr: 0.18 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.632AspAla: 6.632 ± 0.103	0.384AspCys: 0.384 ± 0.023	3.553AspAsp: 3.553 ± 0.076	4.008AspGlu: 4.008 ± 0.066	1.614AspPhe: 1.614 ± 0.037	4.936AspGly: 4.936 ± 0.074	1.384AspHis: 1.384 ± 0.039	2.268AspIle: 2.268 ± 0.047	0.953AspLys: 0.953 ± 0.028	6.134AspLeu: 6.134 ± 0.089	0.875AspMet: 0.875 ± 0.03	1.129AspAsn: 1.129 ± 0.043	4.577AspPro: 4.577 ± 0.07	1.722AspGln: 1.722 ± 0.044	4.675AspArg: 4.675 ± 0.07	2.771AspSer: 2.771 ± 0.055	3.539AspThr: 3.539 ± 0.064	4.671AspVal: 4.671 ± 0.074	0.857AspTrp: 0.857 ± 0.033	1.477AspTyr: 1.477 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.638GluAla: 7.638 ± 0.094	0.398GluCys: 0.398 ± 0.019	2.987GluAsp: 2.987 ± 0.061	5.093GluGlu: 5.093 ± 0.066	1.879GluPhe: 1.879 ± 0.038	4.286GluGly: 4.286 ± 0.071	1.676GluHis: 1.676 ± 0.04	2.954GluIle: 2.954 ± 0.059	1.578GluLys: 1.578 ± 0.049	7.249GluLeu: 7.249 ± 0.079	1.089GluMet: 1.089 ± 0.037	1.207GluAsn: 1.207 ± 0.031	3.357GluPro: 3.357 ± 0.064	2.76GluGln: 2.76 ± 0.063	5.892GluArg: 5.892 ± 0.094	2.722GluSer: 2.722 ± 0.046	3.309GluThr: 3.309 ± 0.061	5.078GluVal: 5.078 ± 0.081	0.98GluTrp: 0.98 ± 0.031	1.35GluTyr: 1.35 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.664PheAla: 3.664 ± 0.059	0.272PheCys: 0.272 ± 0.019	1.892PheAsp: 1.892 ± 0.034	1.36PheGlu: 1.36 ± 0.036	1.006PhePhe: 1.006 ± 0.038	2.858PheGly: 2.858 ± 0.064	0.707PheHis: 0.707 ± 0.029	0.906PheIle: 0.906 ± 0.037	0.405PheLys: 0.405 ± 0.025	2.96PheLeu: 2.96 ± 0.06	0.404PheMet: 0.404 ± 0.021	0.607PheAsn: 0.607 ± 0.028	1.534PhePro: 1.534 ± 0.036	0.697PheGln: 0.697 ± 0.028	1.773PheArg: 1.773 ± 0.042	1.632PheSer: 1.632 ± 0.038	2.245PheThr: 2.245 ± 0.044	2.342PheVal: 2.342 ± 0.056	0.401PheTrp: 0.401 ± 0.023	0.604PheTyr: 0.604 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	8.954GlyAla: 8.954 ± 0.089	0.833GlyCys: 0.833 ± 0.031	4.481GlyAsp: 4.481 ± 0.081	5.057GlyGlu: 5.057 ± 0.068	2.675GlyPhe: 2.675 ± 0.055	7.159GlyGly: 7.159 ± 0.114	1.916GlyHis: 1.916 ± 0.045	3.791GlyIle: 3.791 ± 0.067	2.224GlyLys: 2.224 ± 0.048	8.209GlyLeu: 8.209 ± 0.108	1.931GlyMet: 1.931 ± 0.04	1.655GlyAsn: 1.655 ± 0.041	4.388GlyPro: 4.388 ± 0.075	2.673GlyGln: 2.673 ± 0.059	6.616GlyArg: 6.616 ± 0.091	4.657GlySer: 4.657 ± 0.084	5.36GlyThr: 5.36 ± 0.076	7.399GlyVal: 7.399 ± 0.114	1.455GlyTrp: 1.455 ± 0.046	2.358GlyTyr: 2.358 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	2.695HisAla: 2.695 ± 0.057	0.214HisCys: 0.214 ± 0.014	1.329HisAsp: 1.329 ± 0.046	1.206HisGlu: 1.206 ± 0.029	0.562HisPhe: 0.562 ± 0.028	2.29HisGly: 2.29 ± 0.054	0.754HisHis: 0.754 ± 0.036	0.789HisIle: 0.789 ± 0.029	0.332HisLys: 0.332 ± 0.019	2.283HisLeu: 2.283 ± 0.05	0.31HisMet: 0.31 ± 0.018	0.442HisAsn: 0.442 ± 0.022	1.903HisPro: 1.903 ± 0.047	0.714HisGln: 0.714 ± 0.029	2.166HisArg: 2.166 ± 0.06	1.125HisSer: 1.125 ± 0.037	1.423HisThr: 1.423 ± 0.036	1.764HisVal: 1.764 ± 0.042	0.348HisTrp: 0.348 ± 0.019	0.561HisTyr: 0.561 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.418IleAla: 5.418 ± 0.075	0.313IleCys: 0.313 ± 0.02	2.649IleAsp: 2.649 ± 0.06	2.264IleGlu: 2.264 ± 0.049	0.938IlePhe: 0.938 ± 0.036	3.554IleGly: 3.554 ± 0.072	0.798IleHis: 0.798 ± 0.028	1.569IleIle: 1.569 ± 0.046	0.767IleLys: 0.767 ± 0.028	3.242IleLeu: 3.242 ± 0.054	0.566IleMet: 0.566 ± 0.024	1.024IleAsn: 1.024 ± 0.032	2.382IlePro: 2.382 ± 0.049	0.904IleGln: 0.904 ± 0.037	2.903IleArg: 2.903 ± 0.045	2.141IleSer: 2.141 ± 0.058	2.81IleThr: 2.81 ± 0.058	3.439IleVal: 3.439 ± 0.065	0.357IleTrp: 0.357 ± 0.015	0.771IleTyr: 0.771 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.405LysAla: 2.405 ± 0.063	0.096LysCys: 0.096 ± 0.01	1.021LysAsp: 1.021 ± 0.035	1.408LysGlu: 1.408 ± 0.038	0.464LysPhe: 0.464 ± 0.019	1.564LysGly: 1.564 ± 0.05	0.429LysHis: 0.429 ± 0.022	0.957LysIle: 0.957 ± 0.034	0.731LysLys: 0.731 ± 0.031	1.903LysLeu: 1.903 ± 0.041	0.347LysMet: 0.347 ± 0.019	0.537LysAsn: 0.537 ± 0.024	1.229LysPro: 1.229 ± 0.048	0.644LysGln: 0.644 ± 0.026	1.685LysArg: 1.685 ± 0.044	0.973LysSer: 0.973 ± 0.037	1.201LysThr: 1.201 ± 0.042	1.708LysVal: 1.708 ± 0.047	0.234LysTrp: 0.234 ± 0.014	0.437LysTyr: 0.437 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.471LeuAla: 14.471 ± 0.152	0.892LeuCys: 0.892 ± 0.027	6.384LeuAsp: 6.384 ± 0.073	5.124LeuGlu: 5.124 ± 0.089	2.94LeuPhe: 2.94 ± 0.065	8.527LeuGly: 8.527 ± 0.1	2.297LeuHis: 2.297 ± 0.047	3.837LeuIle: 3.837 ± 0.076	1.743LeuLys: 1.743 ± 0.047	11.746LeuLeu: 11.746 ± 0.16	1.547LeuMet: 1.547 ± 0.048	1.892LeuAsn: 1.892 ± 0.045	6.501LeuPro: 6.501 ± 0.085	2.148LeuGln: 2.148 ± 0.056	9.17LeuArg: 9.17 ± 0.132	5.868LeuSer: 5.868 ± 0.074	6.439LeuThr: 6.439 ± 0.102	9.326LeuVal: 9.326 ± 0.125	1.404LeuTrp: 1.404 ± 0.037	1.866LeuTyr: 1.866 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.212MetAla: 2.212 ± 0.05	0.123MetCys: 0.123 ± 0.01	0.821MetAsp: 0.821 ± 0.028	0.826MetGlu: 0.826 ± 0.027	0.506MetPhe: 0.506 ± 0.02	1.359MetGly: 1.359 ± 0.037	0.308MetHis: 0.308 ± 0.021	0.673MetIle: 0.673 ± 0.025	0.349MetLys: 0.349 ± 0.018	1.802MetLeu: 1.802 ± 0.054	0.284MetMet: 0.284 ± 0.017	0.443MetAsn: 0.443 ± 0.022	1.045MetPro: 1.045 ± 0.03	0.426MetGln: 0.426 ± 0.021	1.588MetArg: 1.588 ± 0.034	1.301MetSer: 1.301 ± 0.035	1.368MetThr: 1.368 ± 0.03	1.547MetVal: 1.547 ± 0.039	0.215MetTrp: 0.215 ± 0.017	0.278MetTyr: 0.278 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.245AsnAla: 2.245 ± 0.043	0.171AsnCys: 0.171 ± 0.012	1.028AsnAsp: 1.028 ± 0.032	1.084AsnGlu: 1.084 ± 0.036	0.539AsnPhe: 0.539 ± 0.023	1.85AsnGly: 1.85 ± 0.052	0.442AsnHis: 0.442 ± 0.022	0.829AsnIle: 0.829 ± 0.03	0.403AsnLys: 0.403 ± 0.022	1.907AsnLeu: 1.907 ± 0.047	0.334AsnMet: 0.334 ± 0.016	0.457AsnAsn: 0.457 ± 0.021	1.666AsnPro: 1.666 ± 0.048	0.657AsnGln: 0.657 ± 0.026	1.496AsnArg: 1.496 ± 0.046	1.024AsnSer: 1.024 ± 0.032	1.162AsnThr: 1.162 ± 0.037	1.463AsnVal: 1.463 ± 0.04	0.296AsnTrp: 0.296 ± 0.017	0.479AsnTyr: 0.479 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.215ProAla: 7.215 ± 0.111	0.353ProCys: 0.353 ± 0.017	4.312ProAsp: 4.312 ± 0.069	4.973ProGlu: 4.973 ± 0.073	1.572ProPhe: 1.572 ± 0.041	5.708ProGly: 5.708 ± 0.091	1.601ProHis: 1.601 ± 0.046	1.737ProIle: 1.737 ± 0.044	1.19ProLys: 1.19 ± 0.037	5.664ProLeu: 5.664 ± 0.07	0.965ProMet: 0.965 ± 0.03	1.125ProAsn: 1.125 ± 0.037	4.221ProPro: 4.221 ± 0.101	2.034ProGln: 2.034 ± 0.044	4.318ProArg: 4.318 ± 0.071	3.32ProSer: 3.32 ± 0.086	3.307ProThr: 3.307 ± 0.064	5.23ProVal: 5.23 ± 0.065	0.958ProTrp: 0.958 ± 0.036	1.368ProTyr: 1.368 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.763GlnAla: 3.763 ± 0.069	0.202GlnCys: 0.202 ± 0.016	1.091GlnAsp: 1.091 ± 0.032	1.957GlnGlu: 1.957 ± 0.052	0.674GlnPhe: 0.674 ± 0.027	2.182GlnGly: 2.182 ± 0.043	0.794GlnHis: 0.794 ± 0.026	1.392GlnIle: 1.392 ± 0.04	0.604GlnLys: 0.604 ± 0.022	3.235GlnLeu: 3.235 ± 0.051	0.604GlnMet: 0.604 ± 0.019	0.512GlnAsn: 0.512 ± 0.026	1.966GlnPro: 1.966 ± 0.052	1.411GlnGln: 1.411 ± 0.056	3.194GlnArg: 3.194 ± 0.052	1.298GlnSer: 1.298 ± 0.038	1.441GlnThr: 1.441 ± 0.041	2.631GlnVal: 2.631 ± 0.049	0.536GlnTrp: 0.536 ± 0.022	0.557GlnTyr: 0.557 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.203ArgAla: 9.203 ± 0.126	0.651ArgCys: 0.651 ± 0.031	4.575ArgAsp: 4.575 ± 0.069	5.049ArgGlu: 5.049 ± 0.082	2.384ArgPhe: 2.384 ± 0.054	5.716ArgGly: 5.716 ± 0.069	2.039ArgHis: 2.039 ± 0.043	3.985ArgIle: 3.985 ± 0.054	1.904ArgLys: 1.904 ± 0.044	8.715ArgLeu: 8.715 ± 0.108	1.894ArgMet: 1.894 ± 0.046	1.655ArgAsn: 1.655 ± 0.043	4.731ArgPro: 4.731 ± 0.067	2.417ArgGln: 2.417 ± 0.05	7.951ArgArg: 7.951 ± 0.114	4.562ArgSer: 4.562 ± 0.068	4.759ArgThr: 4.759 ± 0.082	6.343ArgVal: 6.343 ± 0.088	1.352ArgTrp: 1.352 ± 0.044	2.112ArgTyr: 2.112 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	6.252SerAla: 6.252 ± 0.091	0.418SerCys: 0.418 ± 0.018	2.925SerAsp: 2.925 ± 0.063	2.784SerGlu: 2.784 ± 0.048	1.559SerPhe: 1.559 ± 0.054	5.565SerGly: 5.565 ± 0.086	1.106SerHis: 1.106 ± 0.03	1.671SerIle: 1.671 ± 0.049	0.918SerLys: 0.918 ± 0.027	5.041SerLeu: 5.041 ± 0.074	1.037SerMet: 1.037 ± 0.034	0.93SerAsn: 0.93 ± 0.035	3.732SerPro: 3.732 ± 0.078	1.523SerGln: 1.523 ± 0.039	4.057SerArg: 4.057 ± 0.067	3.198SerSer: 3.198 ± 0.066	3.163SerThr: 3.163 ± 0.062	4.034SerVal: 4.034 ± 0.068	0.916SerTrp: 0.916 ± 0.033	1.325SerTyr: 1.325 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	7.785ThrAla: 7.785 ± 0.088	0.432ThrCys: 0.432 ± 0.02	3.332ThrAsp: 3.332 ± 0.056	3.675ThrGlu: 3.675 ± 0.077	1.608ThrPhe: 1.608 ± 0.045	5.732ThrGly: 5.732 ± 0.07	1.21ThrHis: 1.21 ± 0.041	2.265ThrIle: 2.265 ± 0.053	1.118ThrLys: 1.118 ± 0.034	6.023ThrLeu: 6.023 ± 0.083	0.996ThrMet: 0.996 ± 0.03	1.058ThrAsn: 1.058 ± 0.038	4.3ThrPro: 4.3 ± 0.072	1.524ThrGln: 1.524 ± 0.036	3.886ThrArg: 3.886 ± 0.059	2.925ThrSer: 2.925 ± 0.044	3.757ThrThr: 3.757 ± 0.081	6.375ThrVal: 6.375 ± 0.079	0.842ThrTrp: 0.842 ± 0.033	1.17ThrTyr: 1.17 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	10.856ValAla: 10.856 ± 0.127	0.857ValCys: 0.857 ± 0.03	5.529ValAsp: 5.529 ± 0.097	5.372ValGlu: 5.372 ± 0.071	2.638ValPhe: 2.638 ± 0.043	6.543ValGly: 6.543 ± 0.089	1.96ValHis: 1.96 ± 0.04	3.445ValIle: 3.445 ± 0.068	1.483ValLys: 1.483 ± 0.039	9.269ValLeu: 9.269 ± 0.128	1.375ValMet: 1.375 ± 0.04	1.883ValAsn: 1.883 ± 0.047	4.952ValPro: 4.952 ± 0.071	2.072ValGln: 2.072 ± 0.034	6.978ValArg: 6.978 ± 0.088	4.642ValSer: 4.642 ± 0.06	5.436ValThr: 5.436 ± 0.085	8.72ValVal: 8.72 ± 0.11	1.252ValTrp: 1.252 ± 0.036	1.669ValTyr: 1.669 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.592TrpAla: 1.592 ± 0.05	0.141TrpCys: 0.141 ± 0.012	0.745TrpAsp: 0.745 ± 0.027	0.891TrpGlu: 0.891 ± 0.03	0.491TrpPhe: 0.491 ± 0.02	1.085TrpGly: 1.085 ± 0.031	0.374TrpHis: 0.374 ± 0.02	0.583TrpIle: 0.583 ± 0.026	0.311TrpLys: 0.311 ± 0.022	1.835TrpLeu: 1.835 ± 0.046	0.254TrpMet: 0.254 ± 0.018	0.376TrpAsn: 0.376 ± 0.026	0.758TrpPro: 0.758 ± 0.03	0.62TrpGln: 0.62 ± 0.025	1.371TrpArg: 1.371 ± 0.039	0.844TrpSer: 0.844 ± 0.025	0.92TrpThr: 0.92 ± 0.025	1.059TrpVal: 1.059 ± 0.032	0.378TrpTrp: 0.378 ± 0.024	0.326TrpTyr: 0.326 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.621TyrAla: 2.621 ± 0.058	0.175TyrCys: 0.175 ± 0.013	1.348TyrAsp: 1.348 ± 0.047	1.342TyrGlu: 1.342 ± 0.042	0.664TyrPhe: 0.664 ± 0.028	2.152TyrGly: 2.152 ± 0.047	0.433TyrHis: 0.433 ± 0.023	0.648TyrIle: 0.648 ± 0.029	0.341TyrLys: 0.341 ± 0.018	2.467TyrLeu: 2.467 ± 0.053	0.233TyrMet: 0.233 ± 0.014	0.406TyrAsn: 0.406 ± 0.02	1.303TyrPro: 1.303 ± 0.049	0.78TyrGln: 0.78 ± 0.031	1.975TyrArg: 1.975 ± 0.056	1.077TyrSer: 1.077 ± 0.04	1.26TyrThr: 1.26 ± 0.03	1.732TyrVal: 1.732 ± 0.051	0.355TyrTrp: 0.355 ± 0.017	0.542TyrTyr: 0.542 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3085 proteins (1025469 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---