Halanaerobium hydrogeniformans (Halanaerobium sp. (strain sapolanicus))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4RLL9|E4RLL9_HALHG Uncharacterized protein OS=Halanaerobium hydrogeniformans OX=656519 GN=Halsa_1508 PE=4 SV=1
MM1 pKa = 7.63NIFKK5 pKa = 10.77NNSDD9 pKa = 3.44GFTLIEE15 pKa = 4.2LLIVIAVLGILASIAIPRR33 pKa = 11.84ISGMSDD39 pKa = 2.76QAKK42 pKa = 8.56DD43 pKa = 3.49TNISVIAGSIRR54 pKa = 11.84TAMEE58 pKa = 3.73VYY60 pKa = 9.27YY61 pKa = 10.36QNNEE65 pKa = 4.06SYY67 pKa = 10.37PAQTDD72 pKa = 3.65INNNWDD78 pKa = 3.97NLDD81 pKa = 3.89DD82 pKa = 4.85ALDD85 pKa = 3.73ILEE88 pKa = 5.25LNSQADD94 pKa = 4.01YY95 pKa = 11.07NISAFDD101 pKa = 3.7YY102 pKa = 10.59SVNGSDD108 pKa = 3.67SYY110 pKa = 11.07EE111 pKa = 3.82IKK113 pKa = 9.95LTSDD117 pKa = 3.57STGKK121 pKa = 9.08TYY123 pKa = 11.1LLSNTGFGEE132 pKa = 4.4EE133 pKa = 3.95TSEE136 pKa = 4.15

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4RLH2|E4RLH2_HALHG Putative pre-16S rRNA nuclease OS=Halanaerobium hydrogeniformans OX=656519 GN=Halsa_1459 PE=3 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84GRR5 pKa = 11.84NKK7 pKa = 10.12SCHH10 pKa = 5.45FCGSDD15 pKa = 2.94KK16 pKa = 11.19TIDD19 pKa = 3.73YY20 pKa = 10.82KK21 pKa = 10.61DD22 pKa = 3.4LRR24 pKa = 11.84TLKK27 pKa = 10.39RR28 pKa = 11.84YY29 pKa = 8.08LTDD32 pKa = 3.36RR33 pKa = 11.84GKK35 pKa = 10.55IMPRR39 pKa = 11.84RR40 pKa = 11.84TTGNCSKK47 pKa = 9.88HH48 pKa = 5.55QRR50 pKa = 11.84DD51 pKa = 2.78ITIAVKK57 pKa = 10.22RR58 pKa = 11.84ARR60 pKa = 11.84AIALLPYY67 pKa = 10.28VKK69 pKa = 10.29EE70 pKa = 4.05

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2250

0

2250

740588

30

3769

329.2

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.092 ± 0.055

0.655 ± 0.014

6.01 ± 0.053

8.491 ± 0.061

4.43 ± 0.041

6.395 ± 0.051

1.538 ± 0.019

8.982 ± 0.045

8.194 ± 0.072

9.983 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.022

5.396 ± 0.043

3.027 ± 0.026

2.93 ± 0.029

3.874 ± 0.038

5.928 ± 0.036

4.273 ± 0.045

5.968 ± 0.037

0.733 ± 0.016

3.815 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski