Amycolatopsis albispora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344LF67|A0A344LF67_9PSEU Uncharacterized protein OS=Amycolatopsis albispora OX=1804986 GN=A4R43_33150 PE=4 SV=1
MM1 pKa = 7.34LAALACLLTSCGGVAAAATPHH22 pKa = 6.01FVGDD26 pKa = 4.13YY27 pKa = 10.33VWLDD31 pKa = 3.22TDD33 pKa = 4.67RR34 pKa = 11.84NGLQDD39 pKa = 3.83PGEE42 pKa = 4.32PPVEE46 pKa = 4.14GVRR49 pKa = 11.84ATLLDD54 pKa = 3.72ASGTAVEE61 pKa = 4.61TTRR64 pKa = 11.84SGEE67 pKa = 3.67NGRR70 pKa = 11.84YY71 pKa = 9.63LFGDD75 pKa = 3.91LPEE78 pKa = 5.03GSYY81 pKa = 10.37HH82 pKa = 6.31VCFALDD88 pKa = 3.47ALPIHH93 pKa = 6.29VADD96 pKa = 4.25HH97 pKa = 6.76HH98 pKa = 6.25LTSANAGNDD107 pKa = 3.55AVDD110 pKa = 3.9SDD112 pKa = 4.14VDD114 pKa = 3.74PATGCTGPALTATDD128 pKa = 3.87LTLDD132 pKa = 4.27AGLSAPGNEE141 pKa = 4.3LGDD144 pKa = 4.1YY145 pKa = 10.94AWLDD149 pKa = 3.34QDD151 pKa = 5.29ADD153 pKa = 4.02GTQDD157 pKa = 3.57PAEE160 pKa = 4.41LPVEE164 pKa = 4.46GVRR167 pKa = 11.84AALHH171 pKa = 6.61HH172 pKa = 7.1GDD174 pKa = 3.72GTPTGIAVTTGTDD187 pKa = 3.02GRR189 pKa = 11.84YY190 pKa = 7.26TFSNLRR196 pKa = 11.84DD197 pKa = 3.58GTYY200 pKa = 10.42LVCFDD205 pKa = 4.56LDD207 pKa = 3.44GRR209 pKa = 11.84QATTPGAGDD218 pKa = 3.64EE219 pKa = 4.83SVDD222 pKa = 3.79SDD224 pKa = 3.99ADD226 pKa = 3.57PSTGCTEE233 pKa = 3.86AVTVGLGARR242 pKa = 11.84QDD244 pKa = 3.62LTLDD248 pKa = 4.0LGLLPAPGTPAASPP262 pKa = 3.48

Molecular weight: 26.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344L350|A0A344L350_9PSEU Sodium-independent anion transporter OS=Amycolatopsis albispora OX=1804986 GN=A4R43_08005 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.1VRR4 pKa = 11.84KK5 pKa = 9.11SLRR8 pKa = 11.84SLKK11 pKa = 10.28NKK13 pKa = 9.79PGSQVVRR20 pKa = 11.84RR21 pKa = 11.84HH22 pKa = 5.2GKK24 pKa = 7.66TFVINKK30 pKa = 9.18KK31 pKa = 9.34NPKK34 pKa = 9.38FKK36 pKa = 10.76ARR38 pKa = 11.84QGG40 pKa = 3.41

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8085

0

8085

2667710

31

10948

330.0

35.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.471 ± 0.039

0.737 ± 0.006

5.78 ± 0.019

6.017 ± 0.029

2.962 ± 0.013

9.368 ± 0.029

2.219 ± 0.013

3.352 ± 0.017

2.11 ± 0.022

10.832 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.01

1.893 ± 0.017

5.919 ± 0.022

2.759 ± 0.017

7.849 ± 0.033

4.949 ± 0.018

5.874 ± 0.022

8.779 ± 0.023

1.521 ± 0.011

1.979 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski