Ehrlichia canis (strain Jake)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Ehrlichia; canis group; Ehrlichia canis

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3YRX6|Q3YRX6_EHRCJ 5-formyltetrahydrofolate cyclo-ligase OS=Ehrlichia canis (strain Jake) OX=269484 GN=Ecaj_0492 PE=3 SV=1
MM1 pKa = 7.65EE2 pKa = 5.2GNEE5 pKa = 4.01GGGINVSALQSYY17 pKa = 7.72LHH19 pKa = 5.63QQQSAGTSQEE29 pKa = 4.09DD30 pKa = 4.12EE31 pKa = 4.12EE32 pKa = 5.27TVFSVFVFLTWFYY45 pKa = 11.56KK46 pKa = 10.41NVPCYY51 pKa = 10.44LDD53 pKa = 3.87SVTGNISASVTCLGMSLPTTIVLGSEE79 pKa = 3.78MQKK82 pKa = 11.08NIFGLPKK89 pKa = 10.35LLPDD93 pKa = 4.7PQPGDD98 pKa = 3.47ATAGDD103 pKa = 4.08AGGGADD109 pKa = 5.02DD110 pKa = 4.57GAAAGGDD117 pKa = 4.26DD118 pKa = 4.49PDD120 pKa = 5.04AMQLVHH126 pKa = 7.38PDD128 pKa = 3.32DD129 pKa = 5.83PYY131 pKa = 11.72AQDD134 pKa = 4.13NYY136 pKa = 10.17PHH138 pKa = 7.49ADD140 pKa = 3.22QDD142 pKa = 3.7VAAAAGHH149 pKa = 6.04GLDD152 pKa = 5.8DD153 pKa = 4.22VALQQEE159 pKa = 4.79PEE161 pKa = 4.2YY162 pKa = 10.76HH163 pKa = 6.93HH164 pKa = 7.31DD165 pKa = 3.84RR166 pKa = 11.84SPSPTPVTQHH176 pKa = 5.66GNQYY180 pKa = 11.16DD181 pKa = 3.83HH182 pKa = 7.75DD183 pKa = 4.01FHH185 pKa = 8.78

Molecular weight: 19.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q3YRP7|FABH_EHRCJ 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Ehrlichia canis (strain Jake) OX=269484 GN=fabH PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.6GTFQPSRR9 pKa = 11.84IVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.86GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.02MGRR28 pKa = 11.84RR29 pKa = 11.84ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84RR40 pKa = 11.84VLCAA44 pKa = 3.56

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

921

0

921

313982

42

4245

340.9

38.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.442 ± 0.07

1.679 ± 0.038

5.531 ± 0.079

5.609 ± 0.077

4.468 ± 0.056

5.482 ± 0.078

2.093 ± 0.038

9.609 ± 0.124

7.445 ± 0.069

9.584 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.032

6.584 ± 0.082

2.961 ± 0.043

3.311 ± 0.055

3.471 ± 0.055

8.111 ± 0.1

4.859 ± 0.066

6.856 ± 0.081

0.628 ± 0.024

3.894 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski